Haemophilus influenza Boifilm in Intractable Otitis Media
| Project/Area Number |
21791637
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
TAKEI Shin Wakayama Medical University, 医学部, 学内助教 (40347589)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 急性中耳炎 / バイオフィルム / インフルエンザ菌 / バイオフイルム / 中耳炎 |
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| Research Abstract |
In the biofilm state, H.influenzae is much less susceptible to antimicrobial agents compared to the susceptibility in the planktonic state. We clearly showed the biofilm formation of clinical nontypable H.influenza (NTHi) isolates from acute otitis media(AOM) children. The current results suggest that production of biofilm by NTHi would be associated with clinical outcome of AOM. The minimal biofilm eradication concentration assay and crystal violet assay after antimicrobial exposure demonstrated that higher concentration of a certain antimicrobial agent to be effective for biofilms. The MBEC assay would be helpful for the rational selection of antimicrobial agents effective against microbial biofilms and for the screening of new effective antimicrobial compounds.
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Report
(3 results)
Research Products
(13 results)
