Analysis of pneumococcal surface protein and development of pneumococcal vaccine inhibiting colonization in the upper respiratory tract

Research Project

Project/Area Number	21791639
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Otorhinolaryngology
Research Institution	Wakayama Medical University
Principal Investigator	SAKAI Akihiro Wakayama Medical University, 医学部, 博士研究員 (50423950)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	耳科学 / 肺炎球菌 / PspA / ワクチン / 急性中耳炎 / 急性副鼻腔炎 / 耐性化
Research Abstract	We analyzed the pneumococcal surface proteins of pneumococcal strains from children with otitis media by PCR assay. Recombinant pneumococcal surface proteins were made by TA cloning method. Pneumococcal colonization in the nasopharynx was prevented by intranasal immunization with recombinant pneumococcal surface protein in the mouse model. Cross-protective-reactivity between pneumococcal surface protein families were shown in the systemic infection model. Intranasal immunization with pneumococcal surface protein is important about the development of novel vaccines in the near future.

Report

(3 results)