The strategy to gain hearing ability of the deaf model mouse
| Project/Area Number |
21791647
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Juntendo University |
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Principal Investigator |
IIZUKA Takasi Juntendo University, 医学部, 助教 (40372932)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 難聴モデルマウス / 遺伝子導入 / ウイルスベクター / 遺伝子治療 / 難聴治療 / モデルマウス |
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| Research Abstract |
Hereditary deafness affects about 1 in 2,000 children and mutations in the GJB2 gene are the major cause in various ethnic groups. In order to establish the fundamental therapy of congenital deafness, we generated targeted disruption of Gjb2 using Cre recombinase controlled by P0. Using this animal model, we examined the potential of gene therapy in the inner ear, using the homozygous mutant mice and the heterozygous mutant mice. Adeno-associated virus (AAV) vectors carrying the gap junction beta 2 protein (Gjb2) gene were injected into the scala tympani through the round window of the cochlea of the homozygous mutant adult mice. The expression of Cx26 was not seen in the supporting cells and the hearing ability was not improved.
We succeeded in gene introduction to the supporting cells of neonatal mice without hearing loss using adeno-associated virus vectors. We are going to introduce this virus into the Gjb2 knockout mouse in future to cure hereditary deafness.
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] Lipo-PGE1の突発性難聴に対する有効性2010
Author(s)
飯塚崇, 笠井美里, 春山琢男, 峯川明, 齊藤達矢, 林千江里, 八尾亨, 酒井陽子, 一針幸子, 横井尚子, 横井秀格, 古川正幸, 関眞規子, 楠威志, 池田勝久
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Journal Title
耳鼻咽喉科臨床 補126
Pages: 10-14
NAID
Related Report
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[Journal Article] Cochlear outer hair cells in a dominant-negative connexin26 mutant mouse preserve non-linear capacitance in spite of impaired distortion product otoacoustic emission.2009
Author(s)
Minekawa A, Abe T, Inoshita A, Iizuka T, Kakehata S, Narui Y, Koike T, Kamiya K, Okamura HO, Shinkawa H, Ikeda K.
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Journal Title
Neuroscience. 164(3)
Pages: 1312-1319
Related Report
Peer Reviewed
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