Restore vision by transgene of Channelrhodopsin2 on ON-type bipolar cells in genetically blind rat.

• Project/Area Number: 21791664
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Tohoku University
• Principal Investigator: SUGANO Eriko Tohoku University, 国際高等研究教育機構, 助教 (70375210)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 神経再生 / 網膜 / 遺伝子導入 / ウイルス / プロモーター

Research Abstract

I constructed pAAV-Glu6-ChR2 vector that induce ChR2 gene by the metabotropic glutamate receptor (mGlu6) promoter into the on-type bipolar cells. I established the protocol to purify AAV by HPLC. These results showed the same induction efficiency between injection routes and serotypes of AAV.

Research Products

• [Journal Article] Differentiation of neuronal cells from NIH/3T3 fibroblasts under defined conditions. (2011)
  • Author(s): Wang Z, Sugano E, Isago H, Hiroi T, Tmai M, Tomita H.
  • Journal Title: Development Growth and Differentiation In press
  • NAID: 10028202629
  • Peer Reviewed
• [Journal Article] Immune responses to adeno-associated virus type 2 encoding channelrhodopsin-2 in a genetically blind rat model for gene therapy. (2011)
  • Author(s): Sugano E, Isago H, Wang Z, Murayama N, Tamai M, Tomita H.
  • Journal Title: Gene Therapy 18(3) Pages: 266-274
  • Peer Reviewed
• [Journal Article] Immune responses to adeno-associated virus type 2 encoding channelrhodopsin-2 in agenetically blind rat model for gene therapy. (2011)
  • Author(s): Sugano E, Isago H, Wang Z, Murayama N, Tamai M, Tomita H.
  • Journal Title: Gene Therapy Volume: 18(3) Pages: 266-74
  • Peer Reviewed
• [Journal Article] Differentiation of neuronal cells from NIH/3T3 fibroblasts under defined conditions. (2011)
  • Author(s): Wang Z, Sueano E, Isago H, Hiroi T, Tamai M, Tomita H.
  • Journal Title: Development Growth and Differentiation Volume: (In press)
  • Peer Reviewed
• [Journal Article] Dissecting a role for melanopsin in behavioural light aversion reveals a response independent of conventional photoreception. (2010)
  • Author(s): Semo M, Gias C, Ahmado A, Sugano E, Allen AE, Lawrence JM, Tomita H, Coffey PJ, Vugler AA.
  • Journal Title: PLoS One 29;5(11)
  • Peer Reviewed
• [Journal Article] Dissecting a role for melanopsin in behavioural-light aversion reveals a response independent of conventional photoreception. (2010)
  • Author(s): Semo M, Gias C, Ahmado A, Sugano E, Allen AE, Lawrence JM, Tomita H, Coffey PJ, Vugler AA
  • Journal Title: PLoS One Volume: 29;5(11)
  • Peer Reviewed
• [Journal Article] Channelrhodopsin-2 gene transduced into retinal ganglion cells restores functional vision in genetically blind rats (2010)
  • Author(s): Tomita H, Sugano E, Isago H, Hiroi T, Wang Z, Ohta E, Tamai M.
  • Journal Title: Experimental Eye Research 90(3) Pages: 429-36
  • Peer Reviewed
• [Journal Article] Molecular determinants differentiating photocurrent properties of two channelrhodopsins from chlamydomonas (2009)
  • Author(s): Wang H, Sugiyama Y, Hikima T, Sugano E, Tomita H, Takahashi T, Ishizuka T, Yawo H.
  • Journal Title: The Journal of Biological Chemistry 284(9) Pages: 5685-5696
  • Peer Reviewed
• [Journal Article] Visual Properties of Transgenic Rats Harboring the Channelrhodopsin-2 Gene Regulated by the Thy-1.2 Promoter (2009)
  • Author(s): Tomita H, Sugano E, Fukazawa Y, Isago H, Sugiyama Y, Hiroi T, Ishizuka T, Mushiake H, Kato M, Hirabayash M, Shigemoto R, Yawo H, Tamai M
  • Journal Title: PLoS ONE 4(11)
  • Peer Reviewed
• [Journal Article] Channelrhodopsins provide a breakthrough insight into strategies for curing blindness (2009)
  • Author(s): Tomita H, Sugano E, Isago H, Tamai M
  • Journal Title: Journal of Genetics 88(4) Pages: 409-15
  • Peer Reviewed
• [Presentation] チャネルロドプシンを用いた視覚再生により得られる視覚の特性と予想される視覚情報について (2011)
  • Author(s): 富田浩史, 菅野江里子, 砂金ひとみ, 王卓, 村山なみえ, 玉井信
  • Organizer: 日本視覚学会
  • Place of Presentation: 工学院大学(東京都)
  • Year and Date: 2011-01-20
• [Presentation] 緑藻類由来遺伝子を用いた視覚再生技術 (2010)
  • Author(s): 富田浩史, 菅野江里子, 砂金ひとみ, 王卓, 村山なみえ, 玉井信
  • Organizer: 日本人工臓器学会
  • Place of Presentation: 国際センター(仙台)
  • Year and Date: 2010-11-20
• [Presentation] Immune responses to adeno-associated virus type 2 encoding channelrhodopsin-2 in a genetically blind rat model for gene therapy (2010)
  • Author(s): 菅野江里子
  • Organizer: (トルコ)大学院講義Department of Molecular biology and Genetics, Bogazici University
  • Place of Presentation: Bogazici University(イスタンブール、トルコ)
  • Year and Date: 2010-10-27
• [Presentation] チャネルロドプシン-2を用いた視覚再生の可能性 (2010)
  • Author(s): 菅野江里子
  • Organizer: 第6回網脈絡膜変性フォーラム
  • Place of Presentation: いわて県民情報交流センター(盛岡)
  • Year and Date: 2010-10-10
• [Presentation] チャネルロドプシ-2を用いた視覚再生の可能性 (2010)
  • Author(s): 菅野江里子
  • Organizer: 第6回網脈絡膜変性フォーラム
  • Place of Presentation: いわて県民情報交流センター(盛岡)
  • Year and Date: 2010-10-10
• [Presentation] NIH 3T3線維芽細胞からの神経細胞の誘導 (2010)
  • Author(s): 王卓, 菅野江里子, 砂金ひとみ, 廣井照, 玉井信, 富田浩史
  • Organizer: 日本動物学会
  • Place of Presentation: 東京大学(東京)
  • Year and Date: 2010-09-25
• [Presentation] NIH 3T3線維芽細胞からの神経細胞の誘導 (2010)
  • Author(s): 王卓, 菅野江里子, 砂金ひとみ, 廣井照, 玉井信, 富田浩史
  • Organizer: 日本動物学会
  • Place of Presentation: 東京大学(東京都)
  • Year and Date: 2010-09-25
• [Presentation] ボルボックス由来チャネルロドプシン2を導入した遺伝盲ラットの光反応性 (2010)
  • Author(s): 砂金ひとみ, 菅野江里子, 廣井照, 王卓, 玉井信, 富田浩史
  • Organizer: 日本動物学会
  • Place of Presentation: 東京大学(東京)
  • Year and Date: 2010-09-23
• [Presentation] ボルボックス由来チャネルロドプシン2を導入した遺伝盲ラットの光反応性 (2010)
  • Author(s): 砂金ひとみ, 菅野江里子, 廣井照, 王卓, 玉井信, 富田浩史
  • Organizer: 日本動物学会
  • Place of Presentation: 東京大学(東京都)
  • Year and Date: 2010-09-23
• [Presentation] ボルボックス由来チャネルロドプシン-2の網膜における光特性解析 (2010)
  • Author(s): 菅野江里子、砂金ひとみ、王紅霞、王卓、廣井照、玉井信、富田浩史
  • Organizer: 平成22年度「包括脳ネットワーク」夏のワークショップ
  • Place of Presentation: 旧北海道厚生年金会館(札幌)
  • Year and Date: 2010-07-27
• [Presentation] チャネルロドプシン-2トランスジェニックラットの視覚特性 (2010)
  • Author(s): 富田浩史、菅野江里子、深澤有吾、砂金ひとみ、杉山由香、廣井照、石塚徹、虫明元、加藤めぐみ、平林真澄、重本隆一、八尾寛、玉井信
  • Organizer: 平成22年度「包括脳ネットワーク」夏のワークショップ
  • Place of Presentation: 旧北海道厚生年金会館(札幌)
  • Year and Date: 2010-07-27
• [Presentation] 緑藻類チャネルロドプシン遺伝子を用いた視覚再生研究:現状と課題 (2010)
  • Author(s): 富田浩史、菅野江里子
  • Organizer: JRPS栃木県支部 定期総会
  • Place of Presentation: 宇都宮市総合福祉センター(宇都宮市)
  • Year and Date: 2010-06-20
• [Presentation] 視機能再建のための遺伝子治療:現状と課題 (2010)
  • Author(s): 富田浩史、菅野江里子
  • Organizer: JRPS群馬県支部第11回定期総会
  • Place of Presentation: 群馬県社会福祉総合センター(前橋市)
  • Year and Date: 2010-06-19
• [Presentation] 視機能再建のための遺伝子治療:現状と課題 (2010)
  • Author(s): 富田浩史、菅野江里子
  • Organizer: JRPS群馬県支部 第11回定期総会
  • Place of Presentation: 群馬県社会福祉総合センター(前橋市)
  • Year and Date: 2010-06-19
• [Presentation] Visual Responses of Royal College of Surgeons Rats Transferred Modified Volvox Channelrhodopsin-2 Gene. (2010)
  • Author(s): Sugano, E., Tomita, H., Isago, H., Hiroi, T., Wang, Z., Tamai, M.
  • Organizer: Annual meeting Association for Research in Vision and Ophthalmology.
  • Place of Presentation: Convention Center (Fortlauderdale, FL, US)
  • Year and Date: 2010-05-04
• [Presentation] Systematic and Local Responses of Channelrhodopsin-2 Gene Therapy (2010)
  • Author(s): Tomita, H., Sugano, E., Isago, H., Hiroi, T., Wang, Z., Tamai, M.
  • Organizer: Annual meeting Association for Research in Vision and Ophthalmology
  • Place of Presentation: Convention Center (Fortlauderdale, FL, US)
  • Year and Date: 2010-05-04
• [Presentation] Visual Responses of Royal College of Surgeons Rats Transferred Modified Volvox Channelrhodopsin-2 Gene (2010)
  • Author(s): Sugano, E., Tomita, H., Isago, H., Hiroi, T., Wang, Z., Tamai, M.
  • Organizer: Annual meeting Association for Research in Vision and Ophthalmology
  • Place of Presentation: Convention Center (Fortlauderdale, FL, US)
  • Year and Date: 2010-05-04
• [Presentation] Optimization of volvox-derived channelrhodopsin-1 to express in mammalian cells. (2010)
  • Author(s): Sugano, E., Tomita, H., Isago, H., Hiroi, T., Wang, Z., Tamai, M.
  • Organizer: XIVth International Symposium on Retinal Degeneration.
  • Place of Presentation: Mont-Tremblant(Quebec, Canada)
• [Presentation] Retinal and systemic immune responses after the transfer of channelrhodopsin-2 into RCS rats (2010)
  • Author(s): Hiroshi Tomita, Eriko Sugano, Hitomi Isago, Teru Hiroi, Zhuo Wang, Makoto Tamai
  • Organizer: XIVth International Symposium on Retinal Degeneration
  • Place of Presentation: Mont-Tremblant (Quebec, Canada)
• [Presentation] Optimization of volvox-derived channelrhodopsin-1 to express in mammalian cells (2010)
  • Author(s): Sugano, E., Tomita, H., Isago, H., Hiroi, T., Wang, Z., Tamai, M.
  • Organizer: XIVth International Symposium on Retinal Degeneration
  • Place of Presentation: Mont-Tremblant (Quebec, Canada)
• [Presentation] カニクイサルを用いた視機能評価 (2009)
  • Author(s): 菅野江里子、富田浩史、姫志剛、松坂昌哉、虫明元、砂金ひとみ、廣井照、玉井信
  • Organizer: 第63回日本臨床眼科
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2009-10-10
• [Presentation] チャネルロドプシンの遺伝子導入によって得られる視覚 (2009)
  • Author(s): 富田浩史、菅野江里子、砂金ひとみ、廣井照、玉井信
  • Organizer: 専門別研究会:生体材料研究会(第63回臨床眼科学会)
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2009-10-09
• [Presentation] チャネルロドプシンの遺伝子導入によって得られる視覚 (2009)
  • Author(s): 富田浩史、菅野江里子、砂金ひとみ、廣井照、玉井信
  • Organizer: 専門別研究会:生体材料研究会(第63回日本臨床眼科学会)
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2009-10-09
• [Presentation] チャネルロドプシンによって得られる視覚特性 (2009)
  • Author(s): 菅野江里子、富田浩史、砂金ひとみ、廣井照、玉井信
  • Organizer: 日本色素変性症協会主催世界網膜の日in神奈川
  • Place of Presentation: 神奈川県民センター
  • Year and Date: 2009-10-02
• [Presentation] カニクイザルを用いた視機能評価 (2009)
  • Author(s): 菅野江里子、富田浩史、姫志剛、砂金ひとみ、廣井照、松坂昌哉、虫明元、玉井信
  • Organizer: 日本動物学会
  • Place of Presentation: グランシップ静岡
  • Year and Date: 2009-09-19
• [Presentation] Channelrhodopsin-2 トランスジェニックラットの視機能について (2009)
  • Author(s): 砂金ひとみ, 富田浩史, 菅野江里子, 廣井照, 王卓, 玉井信
  • Organizer: 日本動物学会
  • Place of Presentation: グランシップ静岡
  • Year and Date: 2009-09-17
• [Presentation] 視機能再建の為の遺伝子治療、チャネルロドプシン2によって得られる視覚特性 (2009)
  • Author(s): 富田浩史、菅野江里子、玉井信
  • Organizer: JRPS宮城県支部総会・医療講演会
  • Place of Presentation: 仙台福祉プラザ
  • Year and Date: 2009-07-05
• [Presentation] 緑藻由来遺伝子を利用した視覚再生研究 (2009)
  • Author(s): 富田浩史、菅野江里子、玉井信
  • Organizer: JRPS(日本網膜色素変性症協会)新潟支部医療講演会
  • Place of Presentation: 新潟市総合福会館
  • Year and Date: 2009-06-07
• [Presentation] Visual Function of Wild Type and Channelrhodopsin-2 Transgenic Rats (2009)
  • Author(s): E.Sugano, H.Tomita, H.Isago, H.Yawo, Y.Fukazawa, R.Shigemoto, T.Hiroi, M.Kato, M.Hirabayashi, M.Tamai
  • Organizer: 2009 ARVO annual meeting
  • Place of Presentation: Convention Center, Fortlauderdale, FL, U.S.A
  • Year and Date: 2009-05-03
• [Presentation] Properties of Recovered Visual Function in the Channelrhodopsin-2 Transferred Royal College of Surgeons Rats (2009)
  • Author(s): H.Tomita, E.Sugano, H.Isago, T.Hiroi, H.Yawo, M.Tamai
  • Organizer: 2009 ARVO annual meeting
  • Place of Presentation: Convention Center, Fortlauderdale, FL, U.S.A
  • Year and Date: 2009-05-03
• [Book] 会報誌「あぁるぴい」(研究のテーマ紹介、患者会員へのメッセージ, No.82, 8月号) (2009)
  • Author(s): 菅野江里子
  • Total Pages: 7
  • Publisher: 日本網膜色素変性症協会(JRPS)
• [Book] 講演録No.2『チャネルロドプシンを用いた視覚再生の現状と、今後の課題』得々講座・医療講演会(冊子) (2009)
  • Author(s): 富田浩史、菅野江里子、玉井信
  • Publisher: JRPS
• [Remarks] ホームページ等
  • URL: http://www.visual-neuroscience.med.tohoku.ac.jp/
• [Remarks]
  • URL: http://www.visual-neuroscience.med.tohoku.ac.jp/
• [Remarks]
  • URL: http://www.visual-neuroscience.med.tohoku.ac.jp/
• [Patent(Industrial Property Rights)] (2010)
  • Inventor(s): 富田浩史、菅野江里子
  • Industrial Property Rights Holder: 富田浩史、菅野江里子
  • Filing Date: 2010-07-10
• [Patent(Industrial Property Rights)] 発現効率が改善された光受容チャネルロドプシン (2010)
  • Inventor(s): 富田浩史、菅野江里子
  • Industrial Property Rights Holder: 富田浩史、菅野江里子
  • Filing Date: 2010-07-10
• [Patent(Industrial Property Rights)] 発現効率が改善された光受容チャネルロドプシン (2009)
  • Inventor(s): 富田浩史、菅野江里子
  • Industrial Property Rights Holder: 富田浩史、菅野江里子
  • Filing Date: 2009-08-10