The functional analysis of the vitreous derived cells for the new therapeutic strategy of the intraocular proliferative diseases.

• Project/Area Number: 21791668
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Yamagata University
• Principal Investigator: NISHITSUKA Koichi Yamagata University, 医学部, 助教 (60422169)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 硝子体細胞 / 眼内増殖性疾患 / ヒアルロナン / 糖尿病網膜症 / 増殖硝子体網膜症 / 眼細胞生物学 / 増殖糖尿病網膜症 / 増殖性硝子体網膜 / 4-メチルンベリフェロン / アポトーシス / TNF-alpha / 共培養 / サイトカイン / 血管内皮細胞 / 細胞株

Research Abstract

We established the human vitreous tissue-derived cell strain to investigate the relationship between vitreous derived cells and intraocular proliferative diseases. Inflammatoly cytokines increased in intraocular proliferative diseases induced the hyaluronan production regulation on porcine vitreous derived cells. These cells also enhance the proliferation of the vascular endothelial cells on co-culture system.

Research Products

• [Journal Article] Association of PDGF-BB-induced thrombomodulin with the regulation of inflammation in the corneal and scleral stroma. (2010)
  • Author(s): Namba H, Kashiwagi Y, Nishitsuka K, Takamura H, Yamamoto T, Yamashita H.
  • Journal Title: Invest Ophthalmol Vis Sci. 51(11) Pages: 5460-5469
  • Peer Reviewed
• [Journal Article] Interactions between vitreous-derived cells and vascular endothelial cells in vitreoretinal diseases (2010)
  • Author(s): Tojo N, Kashiwagi Y, Nishitsuka K, Yamamoto S, Asao H, Sugawara N, Yamashita T, Yamamoto T, Yamashita H.
  • Journal Title: Acta Ophthalmol. 88(5) Pages: 564-570
  • Peer Reviewed
• [Journal Article] Cotylenin A inhibits cell proliferation and induces apoptosis and PAX6 mRNA transcripts in retinoblastoma cell lines. (2010)
  • Author(s): Kashiwagi Y, Kato N, Sassa T, Nishitsuka K, Yamamoto T, Takamura H, Yamashita H.
  • Journal Title: Mol Vis. 16 Pages: 970-982
  • Peer Reviewed
• [Journal Article] 増殖因子によるヒアルロナン代謝の調節 (2010)
  • Author(s): 西塚弘一
  • Journal Title: あたらしい眼科 27巻 Pages: 1415-1416
  • NAID: 10028246372
• [Journal Article] 眼科医にもわかる生理活性物質と眼疾患の基本TGF-β (2010)
  • Author(s): 西塚弘一
  • Journal Title: 臨床眼科 64巻 Pages: 2020-2023
• [Journal Article] Cotylenin A inhibits cell proliferation and induces apoptosis and PAX6 mRNA transcripts in retinoblastoma cell lines. (2010)
  • Author(s): Kashiwagi Y, Kato N, Sassa T, Nishitsuka K, Yamamoto T, Takamura H, Yamashita H.
  • Journal Title: Mol Vis. Volume: 16 Pages: 970-982
  • Peer Reviewed
• [Journal Article] Association of PDGF-BB-induced thrombomodulin with the regulation of inflammation in the corneal and scleral stroma. (2010)
  • Author(s): Namba H, Kashiwagi Y, Nishitsuka K, Takamura H, Yamamoto T, Yamashita H.
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 51 Pages: 5460-5469
  • Peer Reviewed
• [Journal Article] 増殖因子によるヒアルロナン代謝の調節 (2010)
  • Author(s): 西塚弘一
  • Journal Title: あたらしい眼科 Volume: 27 Pages: 1415-1416
  • NAID: 10028246372
• [Journal Article] 眼科医にもわかる生理活性物質と眼疾患の基本TGF-β (2010)
  • Author(s): 西塚弘一
  • Journal Title: 臨床眼科 Volume: 64 Pages: 2020-2023
• [Journal Article] Cloning and characterization of human vitreous tissue-derived cells (2010)
  • Author(s): Kashiwagi Y, Nishitsuka K, Takamura H, Yamamoto T, Yamashita H
  • Journal Title: Acta Ophthalmologica Scandinavica
  • Peer Reviewed
• [Journal Article] Cloning and characterization of human vitreous tissue-derived cells (2009)
  • Author(s): Kashiwagi Y, Nishitsuka K, Takamura H, Yamamoto T, Yamashita H
  • Journal Title: Acta Ophthalmol.
  • Peer Reviewed
• [Journal Article] Interactions between vitreous-derived cells and vascular endothelial cells in vitreoretinal diseases (2009)
  • Author(s): Tojo N, Kashiwagi Y, Nishitsuka K, Yamamoto S, Asao H, Sugawara N, Yamashita T, Yamamoto T, Yamashita H.
  • Journal Title: Acta Ophthalmologica Scandinavica
  • Peer Reviewed
• [Presentation] Peripapillary Detachment in Pathogenic Myopiaに対する硝子体手術の長期経過 (2010)
  • Author(s): 西塚弘一、山本禎子、山下英俊
  • Organizer: 第64回日本臨床眼科学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2010-11-13
• [Presentation] Intraoperative fluoroscopy-guided removal of the orbital foreign body in patient with penetrating ocular injury.: a case report. (2010)
  • Author(s): Nishitsuka K, Konno N, Takamura H, Yamamoto T, Yamashita H.
  • Organizer: 25th Asia Pacific Academy of Ophthalmology
  • Place of Presentation: Beijing(中国)
  • Year and Date: 2010-09-18
• [Presentation] Effect of tumor necrosis factor-alpha on hyaluronan production and cell proliferation by porcine vitreous derived cell line. (2010)
  • Author(s): Nishitsuka K, Kashiwagi Y, Kaneko Y, Yamamoto T, Heldin P, Yamashita H.
  • Organizer: 8th International Conference on Hyaluronan
  • Place of Presentation: 京都
  • Year and Date: 2010-06-09
• [Presentation] Immortalization of Several Human Eye-Derived Cells Using Human Papilloma Virus 16 E6 and E7. (2010)
  • Author(s): Nishitsuka K, Kashiwagi Y, Kanno C, Yamamoto T, Takamura H, Yamashita H.
  • Organizer: 83st The Association for Research in Vision and Ophthalmology Annual meeting
  • Place of Presentation: Fort Lauderdale(アメリカ)
  • Year and Date: 2010-05-03
• [Presentation] ヒト眼組織由来不死化線維芽細胞株の性質について (2010)
  • Author(s): 西塚弘一、柏木佳子、菅野智華子、山本禎子、高村浩、山下英俊
  • Organizer: 第114回日本眼科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2010-04-15
• [Presentation] 視力予後が良好であった網膜細動脈瘤破裂後の黄斑部出血に合併した黄斑円孔の1例 (2010)
  • Author(s): 西塚弘一、山本禎子、山下英俊
  • Organizer: 第34回日本眼科手術学会総会
  • Place of Presentation: 京都
  • Year and Date: 2010-01-28
• [Presentation] ヒト硝子体組織由来cellstrainの樹立とブタ硝子体組織由来細胞株との比較検討 (2009)
  • Author(s): 西塚弘一
  • Organizer: 第113回日本眼科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-16
• [Presentation] ヒト硝子体組織由来 cell strain の樹立とブタ硝子体組織由来細胞株との比較検討 (2009)
  • Author(s): 西塚弘一, ほか
  • Organizer: 第113回日本眼科学会総会
  • Place of Presentation: 東京国際フォーラム(東京)
• [Remarks] 山形大学研究者情報
  • URL: http://yudb.kj.yamagata-u.ac.jp/OUTSIDE?ISTActId=SCHKOB0010RIni001&userId=100000187&lang_kbn=0