Unfolded protein response in age related macular degeneration and retinal degeneration.

• Project/Area Number: 21791677
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Nagoya University
• Principal Investigator: KACHI Shu Nagoya University, 医学部附属病院, 講師 (30345904)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 小胞体ストレス / アミロイドβ / アポトーシス / カスパーゼ12 / カスパーゼ4 / GADD153 / CHOP / 4-フェニル酪酸 / ARPE-19 / caspase 12 / caspase 4 / 網膜色素上皮 / 加齢黄斑変性 / 血管内皮増殖因子 / GRP78 / Bip

Research Abstract

It is reported that amyloid beta stimulates the vascular endothelial growth factor (VEGF) expression from the retinal pigment epithelium (RPE) cells. We found that amyloid beta also increases the expression of the unfolded protein response (UPR) markers. These increases of VEGF and UPR were down-regulated by addition of 4-phenylbutyric acid (PBA), UPR inhibitor, to the medium.

Research Products

• [Journal Article] Suppression of choroidal neovascularization and quantitative and qualitative inhibition of VEGF and CCL2 by heparin. (2011)
  • Author(s): Tomida D, Nishiguchi KM, Kataotaoka K, Yasuma TR, Iwata E, Uetani R, Kachi S, Terasaki H.
  • Journal Title: Invest Ophthalmol Vis Sci. in press
  • Peer Reviewed
• [Journal Article] The roles of vitreal macrophages and circulating leukocytes in retinal neovascularization. (2011)
  • Author(s): Kataoka K, Nishiguchi KM, Kaneko H, van Rooijen N, Kachi S, Terasaki H.
  • Journal Title: Invest Ophthalmol Vis Sci. 52 Pages: 1431-1438
  • Peer Reviewed
• [Journal Article] Suppression of choroidal neovascularization and quantitative and qualitative inhibition of VEGF and CCL2 by heparin. (2011)
  • Author(s): Tomida D
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: (印刷中 未定)
  • Peer Reviewed
• [Journal Article] The roles of vitreal macrophages and circulating leukocytes in retinal neovascularization. (2011)
  • Author(s): Kataoka K
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 52 Pages: 1431-1438
  • Peer Reviewed
• [Journal Article] Transfer of Gene to Human Retinal Pigment Epithelial Cells Using Magnetite Cationic Liposomes. (2010)
  • Author(s): Fujii Y, Kachi S, Kawasumi T, Honda H, Ito A, Terasaki H.
  • Journal Title: Br J Ophthalmol 94(8) Pages: 1074-1077
  • Peer Reviewed
• [Journal Article] Paraneoplastic retinopathy associated with retroperitoneal liposarcoma. (2010)
  • Author(s): Kondo M, Mokuno K, Uemura A, Kachi S, Nakamura M, Kondo A, Terasaki H.
  • Journal Title: Clin Ophthalmol. 26;4 Pages: 243-245
  • Peer Reviewed
• [Journal Article] Regression of Macular Edema Secondary to Branch Retinal Vein Occlusion during Anti-TNF-.Therapy for Rheumatoid Arthritis. (2010)
  • Author(s): Kachi S, Kobayashi K, Ushida H, Ito Y, Kondo M, Terasaki H.
  • Journal Title: J Clinical Ophthalmol. 30;4 Pages: 667-670
  • Peer Reviewed
• [Journal Article] Regulation of pathologic retinal angiogenesis in mice and inhibition of VEGF-VEGFR2 binding by soluble heparan sulfate. (2010)
  • Author(s): Nishiguchi KM, Kataoka K, Kachi S, Komeima K, Terasaki H.
  • Journal Title: PLoS One. 20;5(10) Pages: 13493-13493
  • Peer Reviewed
• [Journal Article] A case of acute retinal necrosis after corticosteroid pulse therapy for unilateral Vogt-Koyanagi-Harada disease. (2010)
  • Author(s): Yokoi R, Asami T, Kachi S, Ito Y, Nakamura M, Terasaki H.
  • Journal Title: Retinal Cases & Brief Reports. 4(1) Pages: 51-54
  • Peer Reviewed
• [Journal Article] Transfer of gene to human retinal pigment epithelial cells using magnetite cationic liposomes. (2010)
  • Author(s): Fujii Y
  • Journal Title: Br J Ophthalmol. Volume: 94 Pages: 1074-1077
  • Peer Reviewed
• [Journal Article] Paraneoplastic retinopathy associated with retroperitoneal liposarcoma (2010)
  • Author(s): Kondo M
  • Journal Title: Clin Ophthalmol. Volume: 4 Pages: 243-245
  • Peer Reviewed
• [Journal Article] Regression of macular edema secondary to branch retinal vein occlusion during anti-TNF-alpha therapy for rheumatoid arthritis. (2010)
  • Author(s): Kachi S
  • Journal Title: Clin Ophthalmol. Volume: 4 Pages: 667-670
  • Peer Reviewed
• [Journal Article] Regulation of pathologic retinal angiogenesis in mice and inhibition of VEGF-VEGFR2 binding by soluble heparan sulfate. (2010)
  • Author(s): Nishiguchi KM
  • Journal Title: PLoS One. Volume: 5
  • Peer Reviewed
• [Journal Article] A case of acute retinal necrosis after corticosteroid pulse therapy for unilateral Vogt-Koyanagi-Harada disease. (2010)
  • Author(s): Yokoi R
  • Journal Title: Retinal Cases & Brief Reports 4 Pages: 51-54
  • Peer Reviewed
• [Journal Article] Transfer of Gene to Human Retinal Pigment Epithelial Cells Using Magnetite Cationic Liposomes. (2010)
  • Author(s): Fujii Y
  • Journal Title: Br J Ophthalmol 94(in press)
  • Peer Reviewed
• [Journal Article] Triamcinolone-assisted removal of internal limiting membrane enhances the effect of vitrectomy for diabetic macular edema. (2009)
  • Author(s): Yamakoshi T, Kachi S, Sugita J, Asami T, Ishikawa K, Ito Y, Terasaki H.
  • Journal Title: Ophthalmic Res. 41(4) Pages: 203-209
  • Peer Reviewed
• [Journal Article] Concentration of Vascular Endothelial Growth Factor in Aqueous Humor of Eyes with Advanced Retinopathy of Prematurity before andafter Intravitreal Injection of Bevacizumab. (2009)
  • Author(s): Ito Nonobe N, Kachi S, Kondo M, Takai Y, Takemoto K, Nakayama A, Hayakawa M, Terasaki H.
  • Journal Title: Retina. 29(5) Pages: 579-585
  • Peer Reviewed
• [Journal Article] Intravitreal injection of bevacizumab for macular edema secondary to branch retinal vein occlusion : results after 12 months and multiple regression analysis. (2009)
  • Author(s): Kondo M, Kondo N, Ito Y, Kachi S, Kikuchi M, Yasuma TR, Ota I, Kensaku M, Terasaki H.
  • Journal Title: Retina. 29(9) Pages: 1242-1248
  • Peer Reviewed
• [Journal Article] Triamcinolone-assisted removal of internal limiting membrane enhances the effect of vitrectomy for diabetic macular edema. (2009)
  • Author(s): Yamakoshi T
  • Journal Title: Ophthalmic Res 41 Pages: 203-209
  • Peer Reviewed
• [Journal Article] Concentration of vascular endothelial growth factor in aqueous humor of eyes with advanced retinopathy of prematurity before and after intravitreal injection of bevacizumab. (2009)
  • Author(s): Nonobe NI
  • Journal Title: Retina 29 Pages: 579-585
  • Peer Reviewed
• [Journal Article] Intravitreal injection of bevacizumab for macular edema secondary to BRVO : Results after 12-months and multiple regression analysis. (2009)
  • Author(s): Kondo M
  • Journal Title: Retina 29 Pages: 1242-1248
  • Peer Reviewed
• [Journal Article] 加齢黄斑変性の新しい治療法. (2009)
  • Author(s): 加地秀
  • Journal Title: 日眼会誌 113 Pages: 479-491
  • NAID: 10026260338
  • Peer Reviewed
• [Presentation] 黄斑円孔術後の網膜神経線維層の一過性肥厚 (2011)
  • Author(s): 日比展昭、近藤峰生、石川浩平、米今敬一、加地秀、伊藤逸毅、寺崎浩子
  • Organizer: 第34回日本眼科手術学会総会
  • Place of Presentation: 京都(一般講演、黄斑円孔)
  • Year and Date: 2011-01-28
• [Presentation] 黄斑円孔術後の網膜神経線維層の一過性肥厚 (2011)
  • Author(s): 日比展昭
  • Organizer: 日本眼科手術学会総会
  • Place of Presentation: 京都、京都国際会館
  • Year and Date: 2011-01-28
• [Presentation] 硝子体手術中の眼内温度変化 (2010)
  • Author(s): 原日輪子、井口優子、岩田英嗣、牛田宏昭、安田俊介、大岩和博、黒土愛、加地秀、上野真治、寺崎浩子
  • Organizer: 名古屋大学眼科集談会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-12-18
• [Presentation] 加齢黄斑変性のQuality of Life (2010)
  • Author(s): 植谷留佳、加地秀、石川浩平、高橋明子、岩田英嗣、牛田宏昭、大岩和博、寺崎浩子
  • Organizer: 名古屋大学眼科集談会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-12-18
• [Presentation] 加齢黄斑変性のQuality of Life (2010)
  • Author(s): 植谷留佳
  • Organizer: 名古屋大学眼科集談会
  • Place of Presentation: 名古屋、鶴友会館
  • Year and Date: 2010-12-18
• [Presentation] 硝子体手術中の眼内温度変化 (2010)
  • Author(s): 原日輪子
  • Organizer: 名古屋大学眼科集談会
  • Place of Presentation: 名古屋、鶴友会館
  • Year and Date: 2010-12-18
• [Presentation] 糖尿病黄斑浮腫における眼内血管内皮増殖因子濃度と網膜電図 (2010)
  • Author(s): 牛田宏昭、加地秀、安田俊介、植谷留佳、朴昌華、近藤峰生、寺崎浩子
  • Organizer: 第16回日本糖尿病眼学会総会
  • Place of Presentation: 大阪(一般口演、診断・治療I)
  • Year and Date: 2010-11-26
• [Presentation] 網膜色素上皮へのアミロイドβ負荷と小胞体ストレス (2010)
  • Author(s): 加地秀、松井朝子、黒土愛、牛田宏昭、藤井康生、寺崎浩子
  • Organizer: 第49回日本網膜硝子体学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-11-26
• [Presentation] 網膜中心静脈閉塞における眼内血管内皮増殖因子濃度と獎液性網膜剥離の関係 (2010)
  • Author(s): 伊島亮、安田俊介、加地秀、近藤峰生、寺崎浩子
  • Organizer: 第49回日本網膜硝子体学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-11-26
• [Presentation] 糖尿病黄斑浮腫における眼内血管内皮増殖因子濃度と網膜電図 (2010)
  • Author(s): 牛田宏昭
  • Organizer: 日本糖尿病眼学会
  • Place of Presentation: 大阪、大阪国際会議場
  • Year and Date: 2010-11-26
• [Presentation] 網膜色素上皮へのアミロイドβ負荷と小胞体ストレス (2010)
  • Author(s): 加地秀
  • Organizer: 日本網膜硝子体学会
  • Place of Presentation: 大阪、大阪国際会議場
  • Year and Date: 2010-11-26
• [Presentation] 網膜中心静脈閉塞における眼内血管内皮増殖因子濃度と奨液性網膜剥離の関係 (2010)
  • Author(s): 伊島亮
  • Organizer: 日本網膜硝子体学会
  • Place of Presentation: 大阪、大阪国際会議場
  • Year and Date: 2010-11-26
• [Presentation] 加齢黄斑変性に対するPDTと抗VEGF療法 (2010)
  • Author(s): 加地秀
  • Organizer: 第31回日本レーザー医学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-11-14
• [Presentation] 加齢黄斑変性に対するPDTと抗VEGF療法 (2010)
  • Author(s): 加地秀
  • Organizer: 日本レーザー医学会総会
  • Place of Presentation: 名古屋、ウインク愛知
  • Year and Date: 2010-11-14
• [Presentation] Stage4未熟児網膜症に対する強膜内陥術の成績 (2010)
  • Author(s): 二村裕紀子、加地秀、野々部典枝、近藤峰生、早川昌弘、寺崎浩子
  • Organizer: 第64回日本臨床眼科学会
  • Place of Presentation: 神戸(一般口演、小児眼科)
  • Year and Date: 2010-11-12
• [Presentation] Stage4未熟児網膜症に対する強膜内陥術の成績 (2010)
  • Author(s): 二村裕紀子
  • Organizer: 日本臨床眼科学会
  • Place of Presentation: 神戸、神戸国際会議場
  • Year and Date: 2010-11-12
• [Presentation] Significant correlations between ERG components and VEGF concentration in aqueous humor in eyes with central retinal vein occlusion. (2010)
  • Author(s): Kondo M, Yasuda S, Kachi S, Piao CH, Ueno S, Terasaki H
  • Organizer: XL VIIIth ISCEV 2010
  • Place of Presentation: Western Australia
  • Year and Date: 2010-11-09
• [Presentation] Significant correlations between ERG components and VEGF concentration in aqueous humor in eyes with central retinalvein occlusion. (2010)
  • Author(s): Kondo M
  • Organizer: ISCEV
  • Place of Presentation: オーストラリア ウェスタンオーストラリア
  • Year and Date: 2010-11-09
• [Presentation] The distribution and characteristics of bone marrow-derived cells in the retina during physiological and pathological angiogenesis (2010)
  • Author(s): Kataoka K, Nishiguchi KM, Kachi S, Terasaki H
  • Organizer: WOC 2010
  • Place of Presentation: Berlin
  • Year and Date: 2010-06-06
• [Presentation] The distribution and characteristics of bone marrow-derived cells in the retina during physiological and pathological angiogenesis. (2010)
  • Author(s): Kataoka K
  • Organizer: World Ophthalmology Congress
  • Place of Presentation: ドイツ ベルリン
  • Year and Date: 2010-06-06
• [Presentation] Effect of Preoperative Intravitreal Bevacizumab on Visual Function for Proliferative Diabetic Retinopathy Eyes. (2010)
  • Author(s): Ushida H, Kachi S, Asami T, Ishikawa K, Kondo M, Terasaki H.
  • Organizer: ARVO 2010 Annual Meeting
  • Place of Presentation: Fort Lauderdale
  • Year and Date: 2010-05-05
• [Presentation] Rebound of Macular Edema After Intravitreal Bevacizumab Therapy in Eyes With Macular Edema Secondary to Branch Retinal Vein Occlusion (2010)
  • Author(s): Yasuda S, Kondo M, Kachi S, Ito Y, Terui T, Terasaki H.
  • Organizer: ARVO 2010 Annual Meeting
  • Place of Presentation: Fort Lauderdale
  • Year and Date: 2010-05-05
• [Presentation] Rebound of Macular Edema After Intravitreal Bevacizumab Therapy in Eyes With Macular Edema Secondary to Branch Retinal Vein Occlusion. (2010)
  • Author(s): Yasuda S
  • Organizer: ARVO annual meeting
  • Place of Presentation: アメリカ フォートローダーデール
  • Year and Date: 2010-05-05
• [Presentation] Effect of Preoperative Intravitreal Bevacizumab on Visual Function for Proliferative Diabetic Retinopathy Eyes. (2010)
  • Author(s): Ushida H
  • Organizer: ARVO annual meeting
  • Place of Presentation: アメリカ フォートローダーデール
  • Year and Date: 2010-05-05
• [Presentation] The Role of Heparan Sulfate inOxgen-Induced Retinopathy. (2010)
  • Author(s): Nishiguchi KM, Kataoka K, Kachi S, H.Terasaki.
  • Organizer: ARVO 2010 Annual Meeting
  • Place of Presentation: Fort Lauderdale
  • Year and Date: 2010-05-04
• [Presentation] The Role of Heparan Sulfate in Oxgen-Induced Retinopathy. (2010)
  • Author(s): Nishiguchi KM
  • Organizer: ARVO annual meeting
  • Place of Presentation: アメリカ フォートローダーデール
  • Year and Date: 2010-05-04
• [Presentation] 青錐体一色型色覚日本人家系の赤緑遺伝子解析 (2010)
  • Author(s): 王春霞、細野克博、川野敏夫、加地秀、中村誠、寺崎浩子、三宅養三、中西伸夫、堀田喜裕、簑島伸生
  • Organizer: 第114回日本眼科学会総会
  • Place of Presentation: 名古屋(一般講演; 網脈絡膜研究(1))
  • Year and Date: 2010-04-16
• [Presentation] 青錐体-色型色覚日本人家系の赤緑遺伝子解析 (2010)
  • Author(s): 王春霞
  • Organizer: 日本眼科学会総会
  • Place of Presentation: 名古屋、名古屋国際会議場
  • Year and Date: 2010-04-16
• [Presentation] 網膜中心静脈閉塞症における眼内血管内皮増殖因子濃度と網膜電図 (2010)
  • Author(s): 安田俊介、加地秀、近藤峰生、牛田宏昭、植谷留佳、照井隆行、朴昌華、寺崎浩子
  • Organizer: 第114回日本眼科学会総会
  • Place of Presentation: 名古屋(一般講演、網膜静脈閉塞症)
  • Year and Date: 2010-04-15
• [Presentation] 網膜中心静脈閉塞症における眼内血管内皮増殖因子濃度と網膜電図 (2010)
  • Author(s): 安田俊介
  • Organizer: 日本眼科学会総会
  • Place of Presentation: 名古屋、名古屋国際会議場
  • Year and Date: 2010-04-15
• [Presentation] 抗TNF-α薬で改善したBRVOに伴う黄斑浮腫の一例 (2010)
  • Author(s): 小林謙信、加地秀、牛田宏明、伊藤逸毅、近藤峰生、寺崎浩子
  • Organizer: 第420回東海眼科学会
  • Place of Presentation: 名古屋市
  • Year and Date: 2010-02-06
• [Presentation] 抗TNF-α薬で改善したBRVOに伴う黄斑浮腫の一例. (2010)
  • Author(s): 小林謙信
  • Organizer: 第420回 東海眼科学会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-02-06
• [Presentation] 増殖糖尿病網膜症におけるbevacizumab術前投与の視機能に対する影響 (2010)
  • Author(s): 牛田宏昭、加地 秀、浅見哲、石川浩平、近藤峰生、寺崎浩子
  • Organizer: 第33回日本眼科手術学会
  • Place of Presentation: 東京
  • Year and Date: 2010-01-22
• [Presentation] 増殖糖尿病網膜症におけるbevacizumab術前投与の視機能に対する影響. (2010)
  • Author(s): 牛田宏昭
  • Organizer: 第33回日本眼科手術学会
  • Place of Presentation: 東京
  • Year and Date: 2010-01-22
• [Presentation] 抗血管新生治療 -基礎と臨床-:新しい抗血管新生治療の開発第48回日本網膜硝子体学会 (2009)
  • Author(s): 加地秀
  • Organizer: 第26回日本眼循環学会、第15回日本糖尿病眼学会 3学会合同シンポジウム
  • Place of Presentation: 名古屋
  • Year and Date: 2009-12-05
• [Presentation] 網膜色素上皮細胞における血管内皮増殖因子発現増加への小胞体ストレスの関与 (2009)
  • Author(s): 松井朝子、加地秀、藤井康生、寺崎浩子
  • Organizer: 第48回日本網膜硝子体学会、第26回日本眼循環学会 合同学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-12-05
• [Presentation] 網膜静脈分枝閉塞の黄斑浮腫に対するベバシズマブ療法後のリバウンド現象 (2009)
  • Author(s): 安田俊介、近藤峰生、照井隆行、上野真治、加地 秀、伊藤逸毅、寺崎浩子
  • Organizer: 第48回日本網膜硝子体学会 第26回日本眼循環学会 合同学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-12-05
• [Presentation] 術前網膜電図と糖尿病黄斑浮腫の手術成績 (2009)
  • Author(s): 牛田宏昭、加地秀、近藤峰生、浅見哲、寺崎浩子
  • Organizer: 第15回日本糖尿病眼学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-12-05
• [Presentation] 新しい抗血管新生治療の開発 (2009)
  • Author(s): 加地秀
  • Organizer: 第48回日本網膜硝子体学会、第26回日本眼循環学会、第15回日本糖尿病眼学会 3学会合同シンポジウム
  • Place of Presentation: 名古屋
  • Year and Date: 2009-12-05
• [Presentation] 網膜静脈分枝閉塞の黄斑浮腫に対するベバシズマブ療法後のリバウンド現象. (2009)
  • Author(s): 安田俊介
  • Organizer: 第48回日本網膜硝子体学会、第26回日本眼循環学会 2学会合同
  • Place of Presentation: 名古屋
  • Year and Date: 2009-12-05
• [Presentation] 術前網膜電図と糖尿病黄斑浮腫の手術成績. (2009)
  • Author(s): 牛田宏昭
  • Organizer: 第15回日本糖尿病眼学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-12-05
• [Presentation] 網膜色素上皮細胞における血管内皮増殖因子発現増加への小胞体ストレスの関与. (2009)
  • Author(s): 松井朝子
  • Organizer: 第48回日本網膜硝子体学会、第26回日本眼循環学会 2学会合同
  • Place of Presentation: 名古屋
  • Year and Date: 2009-12-04
• [Presentation] 網膜静脈分枝閉塞の黄斑浮腫に対するベバシズマブの効果:漿液性網膜剥離の有無による比較 (2009)
  • Author(s): 安田俊介、近藤峰生、照井隆行、上野真治、加地秀、寺崎浩子
  • Organizer: 第63回日本臨床眼科学会
  • Place of Presentation: 福岡
  • Year and Date: 2009-10-10
• [Presentation] 網膜静脈分枝閉塞の黄斑浮腫に対するベバシズマブの効果:漿液性網膜剥離の有無による比較. (2009)
  • Author(s): 安田俊介
  • Organizer: 第63回日本臨床眼科学会
  • Place of Presentation: 福岡
  • Year and Date: 2009-10-10
• [Presentation] バックリング手術に抗VEGF療法を併用した未熟児網膜症の一例 (2009)
  • Author(s): 二村裕紀子、野々部店枝、加地秀、近藤峰生、中沢陽子、寺崎浩子
  • Organizer: 第419回東海眼科学会
  • Place of Presentation: 浜松
  • Year and Date: 2009-06-21
• [Presentation] バックリング手術に抗VEGF療法を併用した未熟児網膜症の一例. (2009)
  • Author(s): 二村裕紀子
  • Organizer: 第419回 東海眼科学会
  • Place of Presentation: 浜松
  • Year and Date: 2009-06-21
• [Presentation] Intravitreal injection of bevacizumab for macular edema secondary to BRVO : Results after 12-months and multiple regression analysis. (2009)
  • Author(s): Terui T, Kondo M, Kondo N, Ito Y, Kachi S, Yasuma T, Kikuchi M, Ota I, Miyake K, Terasaki H
  • Organizer: The 24th Congress of the Asia-Pacific Academy of Ophthalmology
  • Place of Presentation: Bali.
  • Year and Date: 2009-05-16