Development of detecting retinal ganglion cell response using electroretinogram

• Project/Area Number: 21791678
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Nagoya University
• Principal Investigator: UENO Shinji Nagoya University, 医学部附属病院, 助教 (80528670)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ERG / retinal ganglion cell / PhNR / 網膜電図 / 網膜神経節細胞 / マウス / 軸索数 / 視神経障害モデル

Research Abstract

We studied detecting retinal ganglion cell response using ERG in animal models. We detected scotopic threshold response (STR) from -7.2 log cd s m^2 to -4.2 log cd s m^2. The amplitude of STR significantly decreased after optic nerve injury. These results indicated STR is originated from retinal ganglion cells. We also checked mouse photopic negative response (PhNR), which is supposed to be originated from retinal ganglion cells in primate. But our results did not show significant reduction of PhNR after tetrodotoxin injection in mouse eye. This result indicated PhNR of mouse is not useful to detect mouse retinal ganglion cell response. Rabbit ERG showed reduction of PhNR after TTX injection and these data showed PhNR of rabbit eye consisted of retinal ganglion cell function. The origin of PhNR may be different among animal species.

Research Products

• [Journal Article] (多)局所ERGと神経眼科 (2011)
  • Author(s): 上野真治
  • Journal Title: 神経眼科 Volume: (印刷中 未定)
• [Journal Article] 眼科検査の手順ERG (2011)
  • Author(s): 上野真治
  • Journal Title: 眼科ケア Volume: (印刷中 未定)
• [Journal Article] Negative regulation of ciliary length by ciliary male germ cell-associated kinase (Mak) is required for retinal photoreceptor survival. (2010)
  • Author(s): Omori Y, Chaya T, Katoh K, Kajimura N, Sato S, Muraoka K, Ueno S, Koyasu T, Kondo M, Furukawa T.
  • Journal Title: Proc Natl Acad Sci USA. 107(52)(Epub 2010 Dec 8) Pages: 22671-22676
  • Peer Reviewed
• [Journal Article] Analyses of ERG in a patient with intraocular lymphoma. (2010)
  • Author(s): Yasuda C, Ueno S, Kondo M, Kondo N, Piao CH, Terasaki H.
  • Journal Title: Clin Ophthalmol. 26;4 Pages: 301-306
  • Peer Reviewed
• [Journal Article] Prolonged blockade of VEGF receptors does not damage retinal photoreceptors or ganglion cells. (2010)
  • Author(s): Miki A, Miki K, Ueno S, Wersinger DM, Berlinicke C, Shaw GC, Usui S, Wang Y, Zack DJ, Campochiaro PA.
  • Journal Title: J Cell Physiol. 224(1) Pages: 262-272
  • Peer Reviewed
• [Journal Article] Gene expression analysis of embryonic photoreceptor precursor cells using BAC-Crx-EGFP transgenic mouse. (2010)
  • Author(s): Muranishi Y, Sato S, Inoue T, Ueno S, Koyasu T, Kondo M, Furukawa T.
  • Journal Title: Biochem Biophys Res Commun. 12;392(3)(Epub 2010 Jan 6) Pages: 317-322
  • Peer Reviewed
• [Journal Article] Negative regulation of ciliary length by ciliary male germ cell-associated kinase (Mak) is required for retinal photoreceptor survival (2010)
  • Author(s): Omori Yoshihiro
  • Journal Title: Proc Natl Acad Sci U S A.2010 Volume: 107 Pages: 22671-22676
  • Peer Reviewed
• [Journal Article] Prolonged blockade of VEGF receptors does not damage retinal photoreceptors or ganglion cells. (2010)
  • Author(s): Miki A
  • Journal Title: Journal of Cell Physiology Volume: 224 Pages: 262-272
  • Peer Reviewed
• [Journal Article] Gene expression analysis of embryonic photoreceptor precursor cells using BAC-Crx-EGFP transgenic mouse (2010)
  • Author(s): Muranishi, Y.Sato, S.Inoue, T.Ueno, S.Koyasu, T.Kondo, M.Furukawa, T.
  • Journal Title: Biochem Biophys Res Commun (In press)
  • Peer Reviewed
• [Journal Article] TRPM1 is a component of the retinal ON bipolar cell transduction channel in the mGluR6 cascade (2010)
  • Author(s): Koike, C.Obara, T.Uriu, Y.Numata, T.Sanuki, R.Miyata, K.Koyasu, T.Ueno, S.Funabiki, K.Tani, A.Ueda, H.Kondo, M.Mori, Y.Tachibana, M.Furukawa, T.
  • Journal Title: Proc Natl Acad Sci USA 107 Pages: 332-337
  • Peer Reviewed
• [Journal Article] Increased expression of catalase and superoxide dismutase 2 reduces cone cell death in retinitis pigmentosa (2009)
  • Author(s): Usui, S.Komeima, K.Lee, S.Y.Jo, Y.J.Ueno, S.Rogers, B.S.Wu, Z.Shen, J.Lu, L.Oveson, B.C.Rabinovitch, P.S.Campochiaro, P.A.
  • Journal Title: Molcular Therapy 17 Pages: 778-786
  • Peer Reviewed
• [Journal Article] I Supernormal ERG oscillatory potentials in transgenic rabbit with rhodopsin P347L mutation and retinal degeneration (2009)
  • Author(s): Sakai, T.Kondo, M.Ueno, S.Koyasu, T.Komeima, K.Terasaki, H.
  • Journal Title: Invest Ophthalmol Vis Sci 50 Pages: 4402-4409
  • Peer Reviewed
• [Presentation] Focal cone ERG changes during retinal degeneration in rhodopsin Pro347Leu transgenic rabbits. (2010)
  • Organizer: ISCEV 2010
  • Place of Presentation: Australia
  • Year and Date: 2010-11-08
• [Presentation] 動物モデルから学んだことTRPM1欠損マウス (2010)
  • Author(s): 上野真治
  • Organizer: 第58回日本臨床視覚電気生理学会
  • Place of Presentation: 岩手県民情報交流センター
  • Year and Date: 2010-10-08
• [Presentation] Focal Cone ERGs of Rhodopsin Pro347Leu Transgenic Rabbits. (2010)
  • Organizer: ARVO 2010
  • Place of Presentation: USA
  • Year and Date: 2010-05-06
• [Presentation] Focal Cone ERGs of Rhodopsin Pro347Leu Transgenic Rabbits (2010)
  • Author(s): 上野真治
  • Organizer: ARVO 2010
  • Place of Presentation: Fort Lauderdale FL USA
  • Year and Date: 2010-05-04
• [Presentation] ロドプシンP347L遺伝子改変ウサギの網膜部位別局所ERG (2010)
  • Author(s): 上野真治
  • Organizer: 第114回日本眼科学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2010-04-15
• [Presentation] 網膜色素変性症の最新の治療について (2009)
  • Author(s): 上野真治
  • Organizer: 網膜視色素変性症の会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-12-08