| Project/Area Number |
21791681
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Ophthalmology
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| Research Institution | Osaka University |
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Principal Investigator |
HASHIDA Noriyasu Osaka University, 医学部附属病院, 医員 (30456959)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 眼免疫学 / リポソーム / ドラッグデリバリーシステム / micro RNA / 実験的自己免疫性ぶどう膜炎 / 遺伝子発現解析 / microRNA |
|---|
| Research Abstract |
We investigated sequential expression changes in microRNA (miRNA) during the natural course of murine experimental autoimmune uveoretinitis (EAU). Dynamic expression changes of miRNA during EAU were observed from the start of immunization. The expression of most miRNAs changed dramatically from the start of immunization. To search for miRNA that might regulate the inflammatory genes, we examined the potential target genes that might control ocular inflammation in EAU. As a result, up-regulated miR-381 and miR-466b-3-3p expressions, a candidate regulator of the NF-kappaB inhibitor (NFKBIA), down-regulation of miR-322* and miR-465b-5p expression, a regulator of NF-kappaB (NFKBA), occurred on days 5, 15and 20. And also, down-regulation of let-7b* and miR-495, a regulator of IL-10 occurred on days 20 and 30. Dynamic expression changes in miRNAs may be involved in EAU pathogenesis. Our results suggested that changes in the expression levels of specific miRNAs and their candidate target genes are involved in development and remission of EAU.
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