Investigation for the expression and the physiological role of Semaphorin3A in the cornea

• Project/Area Number: 21791686
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Yamaguchi University
• Principal Investigator: MORISHIGE Naoyuki Yamaguchi University, 医学部附属病院, 助教 (40346565)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 角膜 / 神経ガイダンス分子 / semaphorin / 創傷治癒 / Semaphorin 3A / Cornea / Epithelium / Wound healing

Research Abstract

Sema3A expressed at the basal-lateral side of corneal epithelial cells in the epithelial wound closure, suggesting that Sema3A may play a regulatory role in the corneal wound healing process.

Research Products

• [Journal Article] Three-Dimensional Analysis of Collagen Lamellae in the Anterior Stroma of the Human Cornea Visualized by Second Harmonic Generation Imaging Microscopy. (2011)
  • Author(s): Morishige N, Takagi Y, Chikama T, Takahara A, Nishida T
  • Journal Title: Invest Ophthalmol Vis Sci 51 Pages: 911-915
  • Peer Reviewed
• [Journal Article] Three-Dimensional Analysis of Collagen Lamellae in the Anterior Stroma of the Human Cornea Visualized by Second Harmonic Generation Imaging Microscopy. (2011)
  • Author(s): Morishige N
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 51 Pages: 911-915
  • Peer Reviewed
• [Journal Article] Expression of semaphorin 3a in the rat corneal epithelium during wound healing. (2010)
  • Author(s): Morishige N, Ko JA, Morita Y, Nishida T
  • Journal Title: Biochem Biophys Res Commun 395 Pages: 451-457
  • Peer Reviewed
• [Journal Article] Abnormal cytokeratin expression in low-grade conjunctival intraepithelial neoplasia. (2010)
  • Author(s): Morishige N, Teranishi S, Kondo T, Nishida T
  • Journal Title: Clinical and Experimental Ophthalmology 38(9) Pages: 899-900
  • Peer Reviewed
• [Journal Article] Expression of semaphorin 3a in the rat corneal epithelium during wound healing. (2010)
  • Author(s): Morishige N
  • Journal Title: Biochem Biophys Res Commun Volume: 395 Pages: 451-457
  • Peer Reviewed
• [Journal Article] Abnormal cytokeratin expression in low-grade conjunctival intraepithelial neoplasia. (2010)
  • Author(s): Morishige N
  • Journal Title: Clinical and Experimental Ophthalmology Volume: 38 Pages: 899-900
  • Peer Reviewed
• [Journal Article] Semaphorin3A expression in the normal epithelium and wound healing process. (2010)
  • Author(s): Morishige N, Ko JA, Morita Y, Nishida T
  • Journal Title: Biochemical and Biophysical Research Communications (in press)
  • Peer Reviewed
• [Journal Article] Detection of subepithelial fibrosis associated with corneal stromal edema by second harmonic generation imaging microscopy. (2009)
  • Author(s): Morishige N, Yamada N, Teranishi S, Chikama T, Nishida T, Takahara A
  • Journal Title: Invest Ophthalmol Vis Sci 50 Pages: 3145-3150
  • Peer Reviewed
• [Journal Article] Second harmonic generation for visualizing 3-dimensional structure of corneal collagen lamellae. (2009)
  • Author(s): Morishige N, Nishida T, Jester JV
  • Journal Title: Cornea 28(9)
• [Journal Article] Upregulation of zo-1 in cultured human corneal epithelial cells by a peptide (PHSRN) corresponding to the second cell-binding site of fibronectin. (2009)
  • Author(s): Yanai R, Ko JA, Nomi N, Morishige N, Chikama T, Hattori A, Hozumi K, Nomizu M, Nishida T
  • Journal Title: Invest Ophthalmol Vis Sci 50 Pages: 2757-2764
  • Peer Reviewed
• [Journal Article] Disruption of zonula occludens-1 localization in the rabbit corneal epithelium by contact lens-induced hypoxia (2009)
  • Author(s): Yanai R, Ko JA, Morishige N, Chikama T, Ichijima H, Nishida T
  • Journal Title: Invest Ophthalmol Vis Sci 50 Pages: 4605-4610
  • Peer Reviewed
• [Journal Article] Double mutation (R124H, N544S) of tgfbi in two sisters with combined expression of avellino and lattice corneal dystrophies. (2009)
  • Author(s): Yamada N, Kawamoto K, Morishige N, Chikama T, Nishida T, Nishioka M, Okayama N, Hinoda Y
  • Journal Title: Mol Vis 15 Pages: 974-979
  • Peer Reviewed
• [Journal Article] Upregulation of connexin43 expression in corneal fibroblasts by corneal epithelial cells. (2009)
  • Author(s): Ko JA, Yanai R, Morishige N, Takezawa T, Nishida T
  • Journal Title: Invest Ophthalmol Vis Sci 50 Pages: 2054-2060
  • Peer Reviewed
• [Journal Article] Detection of subepithelial fibrosis associated with corneal stromal edema by second harmonic generation imaging microscopy. (2009)
  • Author(s): Morishige N, Yamada N, Teranishi S, Chikama T, Nishida T, Takahara A
  • Journal Title: Investigative Ophthalmology and Visual Science 50 Pages: 3145-3150
  • Peer Reviewed
• [Journal Article] Quantitative evaluation of corneal epithelial oedema by confocal microscopy. (2009)
  • Author(s): Morishige N, Takahashi N, Chikamoto N, Nishida T
  • Journal Title: Clinical and Experimental Ophthalmology 37 Pages: 249-253
  • Peer Reviewed
• [Journal Article] Immunohistofluorescence Analysis of Myofibroblast Transdifferentiation in Human Corneas with Bullous Keratopathy
  • Author(s): Morishige N
  • Journal Title: Cornea Volume: (in press)
  • Peer Reviewed
• [Presentation] 第2次高調波発生を応用した正常角膜および疾患角膜の3次元構造解析 (2011)
  • Author(s): 森重直行
  • Organizer: 第35回角膜カンファランス・第27回日本角膜移植学会
  • Place of Presentation: 東京都,品川プリンスホテル
  • Year and Date: 2011-02-17
• [Presentation] 内皮疾患に対する全層角移植～パーツ移植の時代におけるその必要性～ (2011)
  • Author(s): 森重直行
  • Organizer: 第34回日本眼科手術学会総会
  • Place of Presentation: 京都府,京都国際会議場
  • Year and Date: 2011-01-31
• [Presentation] 内皮疾患に対する全層角膜移植～パーツ移植の時代におけるその必要性～ (2011)
  • Author(s): 森重直行
  • Organizer: 第34回日本眼科手術学会総会
  • Place of Presentation: 京都府,京都国際会議場
  • Year and Date: 2011-01-31
• [Presentation] Three-Dimensional Analysis of Collagen Lamellae in the Anterior Stroma of the Human Cornea Visualized by Second Harmonic Generation Imaging Microscopys (2010)
  • Author(s): Morishige N
  • Organizer: 2010 ARVO Annual Meeting
  • Place of Presentation: Fort Lauderdale, FL,USA
  • Year and Date: 2010-05-05
• [Presentation] Three-Dimensional Analysis of Collagen Lamellae in the Anterior Stroma of the Human Cornea (2010)
  • Author(s): Naoyuki Morishige
  • Organizer: 2010 ARVO Annual Meeting
  • Place of Presentation: Fort Lauderdale, FL, USA
  • Year and Date: 2010-05-05
• [Presentation] 前眼部ヘルペス感染症患者における単純ヘルペスウイルス由来タンパクICPOの発現 (2010)
  • Author(s): 森重直行, 山田直之, 近間泰一郎, 中村義邦, 西田輝夫
  • Organizer: 第114回日本眼科学会総会
  • Place of Presentation: 愛知県,名古屋国際会議場
  • Year and Date: 2010-04-16
• [Presentation] 正常ヒト角膜における実質浅層コラーゲン線維束の3次元構造の評価 (2010)
  • Author(s): 森重直行, 高木雄基, 近間泰一郎, 西田輝夫, 高原淳
  • Organizer: 第34回角膜カンファランス・第26回日本角膜移植学会
  • Place of Presentation: 宮城県,仙台国際センター
  • Year and Date: 2010-02-11
• [Presentation] 創傷治癒過程における神経ガイダンス分子Semaphorin3Aの発現の変化 (2009)
  • Author(s): 森重直行, 高知愛, 西田輝夫
  • Organizer: 第113回日本眼科学会総会
  • Place of Presentation: 東京都,東京国際フォーラム
  • Year and Date: 2009-04-16
• [Presentation] 創傷治癒過程における神経ガイダンス分子Semaphorin 3Aの発現の変化 (2009)
  • Author(s): 森重直行
  • Organizer: 第113回日本眼科学会総会
  • Place of Presentation: 東京都
  • Year and Date: 2009-04-16
• [Remarks] ホームページ等