Search of the prophylaxis of the cornea I endothelium disorder by the TGFβ signal inhibition using the gene introduction

• Project/Area Number: 21791705
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Wakayama Medical University
• Principal Investigator: TAKAYOSHI Sumioka 和歌山県立医科大学, 眼科, 講師 (40433362)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 角膜内皮 / 角膜上皮 / 角膜実質 / 創傷治癒

Research Abstract

Ahealthy endothelium is essential for the maintenance of transparency of the cornea. An alkali burn in the cornea is a clinically serious condition because it damages not only the epithelium and stroma but also the endothelium. During healing after an alkali burn, the fibrous structure is formed in the endothelial layer beneath Descemet' smembrane. Formation of such fibrous structure impairs the physiologic function of the endothelium to maintain transparency. Blocking Smad signal effectively suppresses injury-induced endothelial-mesenchymal transition and fibrogenic reaction by corneal endothelium without impairing repair of wound defect. Strategies that block Smad may be useful for prevension and treatment of fibrogenic disorders in the corneal endothelium.

Research Products

• [Journal Article] Impaired Angiogenic Response in the Cornea of Mice Lacking Tenascin C (2011)
  • Author(s): Sumioka T, Fujita N, Kitano A, Okada Y, Saika S
  • Journal Title: Investigative Ophthalmology and Visual Science Volume: 52 Issue: 5 Pages: 2462-2467
  • DOI: 10.1167/iovs.10-5750
  • Peer Reviewed
• [Journal Article] Endothelial Mesenchynlal Transition : A Therapeutic Target in Retrocorneal Menbrane (2010)
  • Author(s): Takeshi Miyamoto, Takayoshi Sumioka, Shizuya Saika
  • Journal Title: CORNEA Volume: 29 Pages: 52-56
  • Peer Reviewed
• [Journal Article] Suppression of injury-induced epithelial-mesenchymal transition in a mouse lens epithelium lacking tenascin-C (2010)
  • Author(s): Tanaka S, Sumioka T, Fujita N, Kitano A, Okada Y, Yamanaka O, Flanders KC, Miyajima M, Saika S
  • Journal Title: Molecular Vision Volume: 16 Pages: 1194-1205
  • Peer Reviewed
• [Journal Article] Transforming growth factor beta signal transduction : a potential target for maintenance/restoration of transparency of the cornea (2010)
  • Author(s): Saika S, Yamanaka O, Sumioka T, Okada Y, Miyamoto T, Shirai K, Kitano A, Tanaka S
  • Journal Title: Eye Contact Lens Volume: 36 Pages: 286-289
  • Peer Reviewed
• [Journal Article] Endothelial Mesenchymal Transition : A Therapeutic Target in Retrocorneal Menbrane. (2010)
  • Author(s): Takeshi Miyamoto, Takayoshi Sumioka, Shizuya Saika
  • Journal Title: CORNEA Volume: 29 Pages: 52-56
  • Peer Reviewed
• [Journal Article] Suppression of injury-induced epithelial-mesenchymal transition in a mouse lens epithelium lacking tenascin-C. (2010)
  • Author(s): Tanaka S, Sumioka T, Fujita N, Kitano A, Okada Y, Yamanaka O, Flanders KC, Miyajima M, Saika S.
  • Journal Title: Molecular Vision Volume: 16 Pages: 1194-1205
  • Peer Reviewed
• [Journal Article] Transforming growth factor beta signal transduction : a potential target for maintenance/restoration of transparency of the cornea. (2010)
  • Author(s): Saika S, Yamanaka O, Sumioka T, Okada Y, Miyamoto T, Shirai K, Kitano A, Tanaka S.
  • Journal Title: Eye Contact Lens Volume: 36 Pages: 286-289
  • Peer Reviewed
• [Presentation] テネイシンC欠損マウスにおける角膜創傷治癒の障害 (2011)
  • Author(s): 住岡孝吉、田中才一、泉谷愛、藤田識人、岡田由香、雑賀司珠也
  • Organizer: 第一回テネイシンフォーラム
  • Place of Presentation: 三重
  • Year and Date: 2011-11-05
• [Presentation] Roles of TRPVl Signal in Migration of Corneal Epithelium (2011)
  • Author(s): Takayoshi Sumioka, Yuka Okada, Peter Reinach, Norihito Fujita, Masayasu Miyajima, Shizuya Saika
  • Organizer: Corneal Cell Biology
  • Place of Presentation: 和歌山
  • Year and Date: 2011-11-05
• [Presentation] テネイシンC欠損マウスにおける角膜創傷治癒の障害 (2011)
  • Author(s): 住岡孝吉、田中才一、泉谷愛、藤田識人、岡田由香、雑賀司珠也
  • Organizer: 第一回テネイシンフォーラム
  • Place of Presentation: 三重、日本
  • Year and Date: 2011-11-05
• [Presentation] Roles of Trpv1 Signal in Migration of Corneal Epithelium (2011)
  • Author(s): T.Sumioka, Y.Okada, P.S.Reinach, N.Fujita, M.Miyajima, S.Saika
  • Organizer: Cornea Cell Biology in Wakayama
  • Place of Presentation: 和歌山、日本
  • Year and Date: 2011-10-30
• [Presentation] Roles of TRPVl Signal in Wound Healing of Corneal Epithelium (2011)
  • Author(s): Takayoshi Sumioka, Yuka Okada, Peter Reinach, Norihito Fujita, Masayasu Miyajima, Shizuya Saika
  • Organizer: 韓日ショイント角膜カンファランス
  • Place of Presentation: 韓国ソウル
  • Year and Date: 2011-10-14
• [Presentation] Roles of Trpv1 Signal in Wound Healing of Corneal Epithelium (2011)
  • Author(s): T.Sumioka, Y.Okada, P.S.Reinach, N.Fujita, M.Miyajima, S.Saika
  • Organizer: 韓日ジョイント角膜カンファランス
  • Place of Presentation: ソウル、韓国
  • Year and Date: 2011-10-14
• [Presentation] Impaired Angiogenic Response and Wound Healing in the Cornea of Mice Lacking Tenascin C (2011)
  • Author(s): Takayoshi Sumioka, Norihito Fujita, Ai Kitano, Yuka Okada, Shizuya Saika
  • Organizer: オステオポンチン研究会
  • Place of Presentation: 札幌、日本
  • Year and Date: 2011-06-19
• [Presentation] Impaired Angiogenic Response and Wound Healing in the Cornea of Mice Lacking Tenascin C (2011)
  • Author(s): Takayoshi Sumioka, Norihito Fujita, Ai Kitano, Yuka Okada, Shizuya Saika
  • Organizer: オステオポンチン研究会
  • Place of Presentation: 札幌
  • Year and Date: 2011-06-18
• [Presentation] Effect of blocking TGF-beta/Smad signal suppresses epithelial-mesenchymal transition and fibrogenic reaction by corneal endothelium post-alkali-burn in rats (2011)
  • Author(s): Sumioka T, Ikeda K, Okada Y, Yamanaka O, Saika S
  • Organizer: Keystone Symposia
  • Place of Presentation: Vancouver, Canada
• [Presentation] Impaired angiogenic response in cornea by lacking tenascin C in mice (2010)
  • Author(s): Takayoshi Sumioka, Ai Kitano, Norihito Fujita, Yuka Okada, Shizuya Saika
  • Organizer: ARVO
  • Place of Presentation: Florida, USA
  • Year and Date: 2010-05-03
• [Presentation] 角膜創傷治癒過程での角膜血管新生におけるテネイシンCの役割 (2010)
  • Author(s): 住岡孝吉、岡田由香、藤田識人、北野愛、雑賀司珠也
  • Organizer: 日本眼科学会総会
  • Place of Presentation: 名古屋、日本
  • Year and Date: 2010-04-16
• [Presentation] Roles of Trpv1 Signal in Migration of Comeal Endothelium Epitheliurn (2010)
  • Author(s): Takayoshi Sumioka
  • Organizer: Gordon Research Conference
  • Place of Presentation: Ventura, USA
  • Year and Date: 2010-03-08
• [Presentation] Inlpaired angiogenic response in cornea by lacking tenascin C in mice (2010)
  • Author(s): Sumioka T, Kitano A, Fujita N, Okada Y, Saika S
  • Organizer: ARVO
  • Place of Presentation: Florida, USA
• [Presentation] 角膜創傷治癒過程での角膜血管新生におけるテネイシンCの役割 (2010)
  • Author(s): 住岡孝吉、岡田由香、藤田識人、北野 愛、雑賀司珠也
  • Organizer: 日本眼科学会総会
  • Place of Presentation: 名古屋
• [Presentation] Roles of Trpvl Signal in Migration of Corneal Endothelium Epithelium (2010)
  • Author(s): Takayoshi Sumioka, Takayoshi Sumioka, Yuka Okada, Peter Reinach, Akihiro Inoue, Masayasu Miyajima, Shizuya Saika
  • Organizer: Gordon Research Conference
  • Place of Presentation: Ventura,USA
• [Presentation] Roles of Trpv1 Signal in Migration of Corneal Endothelium Epithelium (2009)
  • Author(s): Takayoshi Sumioka
  • Organizer: ARVO
  • Place of Presentation: Florida, USA
  • Year and Date: 2009-05-07
• [Presentation] 家兎角膜内皮創傷治癒過程におけるTRPV-1受容体の役割 (2009)
  • Author(s): 住岡孝吉
  • Organizer: 第113回日本眼科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-16
• [Presentation] Roles of Trpvl Signal in Migration of Corneal Endothelium Epitheliunl (2009)
  • Author(s): Takayoshi Sumioka, Takayoshi Sumioka, Yuka Okada, Peter Reinach, Akihiro Inoue, Masayasu Miyajima, Shizuya Saika
  • Organizer: ARVO
  • Place of Presentation: Florida, USA
• [Presentation] 家兎角膜内皮創傷治癒過程におけるTRPV-1受容体の役割 (2009)
  • Author(s): 住岡孝吉、岡田由香、雑賀司珠也
  • Organizer: 第113回 日本眼科学会総会
  • Place of Presentation: 東京