| Project/Area Number |
21791706
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Ophthalmology
|
|---|
| Research Institution | Wakayama Medical University |
|---|
Principal Investigator |
ITO Takao Wakayama Medical University, 医学部, 助教 (30315931)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 網膜 / 神経ステロイド / 免疫組織化学 / ウエスタンブロット |
|---|
| Research Abstract |
Neurosteroids are synthesized from cholesterol or other steroidal precursors and affect neuronal functions by modulating neurotransmission in the central nervous system. Recent studies have shown that neurosteroids are produced also in the retina. However, it is unclear where the enzymes are located in the retina and how they contribute to retinal physiology. Here, we investigated the localization of steroid metabolizing enzymes in the developing and adult rat retina by using immunohistochemistry with specific antibodies. Furthermore, to evaluate the developing expression of the enzymes in the retina, we carried out reverse transcription-polymerase chain reaction.
|
