Construction of scaffold-free tracheal cartilage using sell sheet-based tissue engineering and dynamic culture
| Project/Area Number |
21791730
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatric surgery
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| Research Institution | Osaka University |
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Principal Investigator |
TANI Gakuto Osaka University, 医学部附属病院, 医員 (60467561)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 再生医療 / 軟骨再生 / 細胞シート / 回転培養 / 再生医学 |
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| Research Abstract |
Auricular chondrocytes were harvested from New Zealand white rabbits and cultivated to form a chondrocyte sheet. The sheet was looped around a silicon tube and cultivated in dynamic conditions. Engineered cartilage maintained the shape and a proper level of rigidity and flexibility, under in vitro conditions. Glycosaminoglycans content of the engineered cartilage corresponded approximately to the content of the rabbit auricular cartilage. Engineered cartilage was easy to convert the cylindrical cartilage into serial ring cartilages by slicing. The graft of engineered cartilage maintained a cylindrical shape, and was elastic and stiff. But, the GAG content of the graft was significantly decreased.
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Report
(3 results)
Research Products
(12 results)
