Cryopreservation and dried preservation at room temperature of human skin by vitrification procedure.

• Project/Area Number: 21791745
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plastic surgery
• Research Institution: Okayama University (2009, 2011); National Research Institute for Child Health and Development (2010)
• Principal Investigator: TOKUYAMA Eijiro 岡山大学, 岡山大学病院, 医員 (90379785)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 皮膚移植 / スキンバンク / クリプトビオシス

Research Abstract

Cryopreservation of biological tissue needs special facilities. Vitrification procedure may solve this problem. It is known that vitrification procedure increases cryopreserved cell survival rate. We carried out cryopreservation and dried preservation at room temperature of human and rat skin by vitrification procedure. Besides, we carried out rat skin grafting. We could confirm cell structure maintain by transmission electron microscopy, but cells could not survive.