| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
Although iPS cells could be generated from most somatic cells, the poor efficiency of cell reprogramming and the uneven quality of iPS cells are still important problems. Here, we generated iPS cells from adult mouse mesenchymal stem cells (MSCs), osteo-progenitors (OP cells) and Tail Tip Fibroblasts (TTFs), and compared the induction efficiency and quality of individual iPS clones. MSCs had a higher reprogramming efficiency compared with OP cells and TTFs. The iPS cells induced from MSCs appeared to be the closest equivalent to ES cells. Our findings suggest that a purified source of undifferentiated cells from adult tissue can produce high-quality iPS cells. In this context, prospectively enriched MSCs are a promising candidate for the efficient generation of high-quality iPS cells.
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