Regulation of mesenchymal stem cells in vivo by endogenous electric fields

• Project/Area Number: 21791755
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plastic surgery
• Research Institution: Keio University
• Principal Investigator: MABUCHI Yo Keio University, 医学部, 助教 (50424172)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 創傷治癒学 / 間葉系幹細胞 / 細胞移植 / 再生医学 / 移植・再生医療 / 細胞・組織 / 幹細胞制御

Research Abstract

Wound healing is essential for maintaining the integrity of multicellular organisms. It is thought that Mesenchymal Stem Cells (MSCs) were concerned with wound healing, however because MSCs have only been isolated from tissue in culture, the equivalent cells have not been identified in vivo. In this study, we performed a comprehensive screening of putative surface markers in order to select those that are most predictive of a pure MSC population in hBM. The transplant of the human MSCs enabled to analyze the migration of the human MSCs after the injury in vivo. Isolation technology of human MSC can analyze pure stem cell itself and elucidation of wound healing by an imaging technology.

Research Products

• [Journal Article] Purified mesenchymal stem cells are an efficient source for iPS cell induction. (2011)
  • Author(s): Kunimichi Niibe, Yoshimi Kawamura, Daisuke Araki, Satoru Morikawa, Kyoko Miura, Sadafumi Suzuki, Shigeto Shimmura, Takehiko Sunabori, Yo Mabuchi, Yasuo Nagai, Taneaki Nakagawa, Hideyuki Okano, Yumi Matsuzaki
  • Journal Title: Plos ONE 6(3)
  • Peer Reviewed
• [Journal Article] Schwann Cell Plasticity After Spinal Cord Injury Shown by Neural Crest Lineage Tracing. (2011)
  • Author(s): Nagoshi N, Shibata S, Nakamura M, Mabuchi Y, Matsuzaki Y, Toyama Y, Okano H
  • Journal Title: Glia 59(5) Pages: 771-784
  • Peer Reviewed
• [Journal Article] Mesp1+ early paraxial mesodermal cells supply initial bone marrow mesenchymal stem cells capable of differentiating into neural crest lineage cells. (2011)
  • Author(s): Niibe K, Morikawa S, Mabuchi Y, Araki D, Nakagawa T, Okano H, Matsuzaki Y
  • Journal Title: Inflammation and Regeneration 31(1) Pages: 116-124
  • NAID: 130004943767
  • Peer Reviewed
• [Journal Article] Dynamics of Delayed P53 Mutations in Mice Given Whole-Body Irradiation at 8 Weeks (2011)
  • Author(s): RYUJI OKAZAKI, AKIRA OOTSUYAMA, HIROYO KAKIHARA, YO MABUCHI, YUMIMATSUZAKI, YUICHIMICHIKAWA, TAKASHI IMAI, TOSHIYUKI NORIMURA
  • Journal Title: Int.J.Radiation Oncology Biol. Phys. 79(1) Pages: 247-254
  • Peer Reviewed
• [Journal Article] DYNAMICS OF DELAYED P53 MUTATIONS IN MICE GIVEN WHOLE-BODY IRRADIATION AT 8 WEEKS (2011)
  • Author(s): Ruji Okazaki, Yo Mabuchi, et al.
  • Journal Title: Int.J.Radiation Oncology Biol.Phys. Volume: 79(1) Pages: 247-54
  • Peer Reviewed
• [Journal Article] Mesp1+ early paraxial mesodermal cells supply initial bone marrow mesenchymal stem cells capable of differentiating into neural crest lineage cells. (2011)
  • Author(s): Kunimichi Niibe, Yo Mabuchi, et al.
  • Journal Title: Inflammation and Regeneration Volume: 31(1) Pages: 116-124
  • NAID: 130004943767
  • Peer Reviewed
• [Journal Article] Schwann Cell Plasticity After Spinal Cord Injury Shown by Neural Crest Lineage Tracing. (2011)
  • Author(s): Nagoshi N, Yo Mabuchi, et al.
  • Journal Title: Glia Volume: 59(5) Pages: 771-84
  • Peer Reviewed
• [Journal Article] Purified mesenchymal stem cells are an efficient source for iPS cell induction. (2011)
  • Author(s): Kunimichi Niibe, Yo Mabuchi, et al.
  • Journal Title: Plos ONE Volume: 6(3)(online)
  • Peer Reviewed
• [Journal Article] The late effects of radiation on lifespan, lymphocyte proliferation and p53 haplodeficiency in mice (2010)
  • Author(s): Ryuji Okazaki, Yo Mabuchi, Yasuhiro Yoshida, Sadafumi Suzuki, Ning Ding, Yumi Matsuzaki, Akira Ootsuyama, Toshiyuki Norimura
  • Journal Title: Int J Radiat Biol. 86(11) Pages: 927-934
  • Peer Reviewed
• [Journal Article] c-MYC overexpression with loss of Ink4a/Arf transforms bone marrow stromal cells into osteosarcoma accompanied by loss of adipogenesis (2010)
  • Author(s): T S himizu, T Ishikawa, E Sugihara S Kuninaka, T Miyamoto, Y Mabuchi, Y Matsuzaki, T Tsunoda, F Miya, H Morioka, R Nakayama, E Kobayashi, Y Toyama, A Kawai, H Ichikawa, T Hasegawa, S Okada, T Ito, Y Ikeda, T Suda, H Saya
  • Journal Title: Oncogene 29(42) Pages: 5687-5699
  • Peer Reviewed
• [Journal Article] The late effects of radiation on lifespan, lymphocyte proliferation and p53 haplodeficiency in mice (2010)
  • Author(s): Ruji Okazaki, Yo Mabuchi, et al.
  • Journal Title: Int J Radiat Biol. Volume: 86(11) Pages: 927-34
  • Peer Reviewed
• [Journal Article] Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow (2009)
  • Author(s): Satoru Morikawa, Yo Mabuchi, et al.
  • Journal Title: The Journal of Experimental Medicine Vol.206 No.11 Pages: 2483-2496
  • Peer Reviewed
• [Presentation] Clonal isolation of human mesenchymal stem cells elucidates heterogeneity within the self-renewal and multi-potent stem cell compartment (2010)
  • Author(s): Yumi Matsuzaki, Yo Mabuchi (発表者), Seiko Harada, Kunimichi Niibe, Hideyuki Okano
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会(口頭・ポスター)
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Clonal isolation of human mesenchymal stem cells elucidates heterogeneity within the self-renewal and multi-potent stem cell compartment (2010)
  • Author(s): Yo Mabuchi
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 兵庫・神戸
  • Year and Date: 2010-12-09
• [Presentation] PROSPECTIVE CLONAL ISOLATION OF HUMAN MESENCHYMAL STEM CELLS ELUCIDATES HETEROGENEITY WITHIN THE MULTI-POTENT STEM CELL COMPARTMENT (2010)
  • Author(s): Yo Mabuchi
  • Organizer: International Society for Stem Cell Research (ISSCR), 8^<th> ANNUAL MEETING
  • Place of Presentation: USA・San Francisco
  • Year and Date: 2010-06-17
• [Presentation] PROSPECTIVE CLONAL ISOLATION OF HUMAN MESENCHYMAL STEM CELLS ELUCIDATES HETEROGENEITY WITHIN THE MULTI-POTENT STEM CELL COMPARTMENT (2010)
  • Author(s): Yo Mabuchi
  • Organizer: 第8回幹細胞シンポジウム
  • Place of Presentation: 兵庫・淡路島
  • Year and Date: 2010-05-13
• [Presentation] FACSを用いたヒト間葉系幹細胞の予期的分離とClonal解析 (2010)
  • Author(s): 馬渕洋
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
  • Year and Date: 2010-03-19
• [Presentation] ヒト間葉系幹細胞の予期的分離とClonal解析 (2010)
  • Author(s): 馬渕洋
  • Organizer: 第6回宮崎サイエンスキャンプ
  • Place of Presentation: 宮崎
  • Year and Date: 2010-02-26
• [Presentation] PROSPECTIVE CLONAL ISOLATION OF HUMAN MESENCHYMAL STEM CELLS ELUCIDATES HETEROGENEITY WITHIN THE MULTI-POTENT STEM CELL COMPARTMENT (2010)
  • Author(s): Mabuchi, Yo (発表者), Morikawa, Satoru, Suzuki, Sadafumi, Lein, Lawrence, Niibe, Kunimichi, Nagai, Yasuo, Sunabori, Takehiko, Okano, Hideyuki, Matsuzaki, Yumi
  • Organizer: International Society for Stem Cell Research (ISSCR), 8th ANNUAL MEETING
  • Place of Presentation: San Francisco
• [Presentation] PROSPECTIVE CLONAL ISOLATION OF HUMAN MESENCHYMAL STEM CELLS ELUCIDATES HETEROGENEITY WITHIN THE MULTI-POTENT STEM CELL COMPARTMENT (2010)
  • Author(s): Mabuchi, Yo(発表者), Morikawa, Satoru, Suzuki, Sadafumi, Lein, Lawrence, Niibe, Kunimichi, Nagai, Yasuo, Sunabori, Takehiko, Okano, Hideyuki, Matsuzaki, Yumi
  • Organizer: 第8回幹細胞シンポジウム
  • Place of Presentation: 淡路島
• [Presentation] Flow cytometric isolation and clonal identification of mesenchymal stem cells in human bone marrow (2009)
  • Author(s): Yo Mabuchi
  • Organizer: International Society for Stem Cell Research (ISSCR), 7^<th> ANNUAL MEETING
  • Place of Presentation: Barcelona
  • Year and Date: 2009-07-09
• [Presentation] Isolation and Identification of Mesenchymal Stem Cells in Human Bone Marrow (2009)
  • Author(s): 馬渕洋
  • Organizer: 第7回幹細胞シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2009-05-15
• [Remarks] ホームページ等
  • URL: http://www.okano-lab.com/ja/publication
• [Remarks]
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