| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
The purpose of this study was to develop the novel therapy for the Bisphosphonate Related Osteonecrosis of the Jaw(BPONJ) with mesenchymal stem cells implantation. To establish the animal model for BPONJ, 1.0mg/kg/each or 2.0mg/kg/each Alendronate(Ald) was injected into the Fischer rat by intraperitoneal injection. After 1 and 2 weeks the upper molar tooth extracted, histological observation was performed with the hematoxylin and eosin staining. The results showed that no significant difference was seen between Ald injection groups and control groups although the bone regeneration in the teeth sockets was slightly delayed in the Ald injection groups.
To examine the bone regeneration ability with adipose-derived stem cells(ASCs), the combination of ASCs and platelet-rich plasma(PRP) was implanted into rat calvarial bone defect model. After 4 and 8 weeks ASCs/PRP implantation, new-formed bone was significantly higher than the other groups in the micro-CT analysis.
This study suggested that the combination of ASCs and PRP was effective method for bone regeneration although the BRONJ animal model was not established.
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