Investigation of molecular mechanism involved in the chain of thrombus and inflammation under whole blood flow

• Project/Area Number: 21791774
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Emergency medicine
• Research Institution: Nara Medical University
• Principal Investigator: FUKUSHIMA Hidetada 奈良県立医科大学, 医学部, 助教 (90347568)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 集中治療医学 / 炎症 / 血栓 / 敗血症 / 炎症反応 / 血栓形成 / VWF propeptide / ADAMTS-13 / VWF Propeptide

Research Abstract

To investigate the association between inflammation and thrombus formation, we observed the variation of von Willebrand Factor propeptide(VWF-pp), which is released from endothelial cell by inflammatory stimuli in sepsis-induced coagulopathy human cases. VWF-pp correlated with the variation of TNF-α, one of the typical inflammatory cytokine. Furthermore, VWF-pp inversely correlated with the ADAMTS-13, which regulates the excessive thrombus formation by cleaving VWF released from endothelial cells by inflammation. The ratio of ADAMTS-13 to VWF-pp was well correlated to the disease severity, such as SOFA score.

Research Products

• [Journal Article] 敗血症性DICに対する遺伝子組み換えトロンボモジュリンの凝固炎症への影響 (2011)
  • Author(s): 矢田憲孝、西尾健治、関匡彦、福島英賢、瓜園泰之、畑倫明、奥地一夫
  • Journal Title: 日本救急医学会雑誌 Volume: 22(9) Pages: 749-757
• [Journal Article] Therapeutic effect of recombinant thrombomodulin on the hemostatic and inflammatory response in patients with septic DIC (2011)
  • Author(s): 矢田憲孝
  • Journal Title: Nihon Kyukyu Igakukai Zasshi Volume: 22 Issue: 9 Pages: 749-757
  • DOI: 10.3893/jjaam.22.749
  • NAID: 130004542321
  • ISSN: 0915-924X, 1883-3772
  • Peer Reviewed
• [Presentation] The ratio of ADAMTS-13 to VWF-Propeptide can reflect the disease severity and the extent of inflammation of the patients with severe sepsis or septic shock. (2011)
  • Author(s): Fukushima H, Nishio K, Watanabe T, Seki T, Matsui H, Sugimoto M, Matsumoto M, Fujimura Y, Okuchi K
  • Organizer: 53^<rd> American Society of Haematology Annual Meeting and Exposition
  • Place of Presentation: San Diego
  • Year and Date: 2011-12-12
• [Presentation] Arecombinat thrombomodulin improves hemostasis disturbance and inflammation in septic patients with DIC (2011)
  • Author(s): Yada N, Nishio K, Fukusima H, Seki T, Urizono Y, Hata M, Asai H, Doi M, Matsui H, Sugimoto M
  • Organizer: 53^<rd> American Society of Haematology Annual Meeting and Exposition
  • Place of Presentation: San Diego
  • Year and Date: 2011-12-12
• [Presentation] A recombinat throrabomodulin improves hemostasis disturbance and inflammation in septic patients with DIC (2011)
  • Author(s): Noritaka Yada
  • Organizer: 53^<rd> American Society of Haematology Annual Meeting and Exposition
  • Place of Presentation: San Diego
  • Year and Date: 2011-12-12
• [Presentation] The ratio of ADAMTS-13 to VWF-Propeptide can reflect the disease severity and the extent of inflammation of the patients with severe sepsis or septic shock (2011)
  • Author(s): Hidetada Fukushima
  • Organizer: 53^<rd> American Society of Haematology Annual Meeting and Exposition
  • Place of Presentation: San Diego
  • Year and Date: 2011-12-12
• [Presentation] Transition of plasma VWF propeptide, VWF and ADAMTS-13 in patients with septic DIC (2011)
  • Author(s): Fukusima H, Nishio K, Okuchi K, Isonish A, Matsumoto M, Fujimura Y, Takano K, Asai H, Watanabe T, Ito S, Seki T, Urizono Y, Hata M, Shida Y, Matsui H, Sugimoto M
  • Organizer: 23^<rd> Congress of the International Society on Thrombosis and Haemostasis
  • Place of Presentation: Kyoto
  • Year and Date: 2011-07-26
• [Presentation] Therapeutic effect of recombinant thrombomodulin on the hemostasis and inflammatory response in patients with septic DIC (2011)
  • Author(s): Yada N, Nishio K, Asai H, Watanabe T, Seki T, Ueyama T, Fukushima H, Urizono Y, Hata M, Okuchi K
  • Organizer: 23^<rd> Congress of the International Society on Thrombosis and Haemostasis
  • Place of Presentation: Kyoto
  • Year and Date: 2011-07-26
• [Presentation] Therapeutic effect of recorabinant throrabomodulin on the hemostasis and inflammatory response in patients with septic DIC (2011)
  • Author(s): Noritaka Yada
  • Organizer: 23^<rd> Congress of the International Society on Thrombosis and Haemostasis
  • Place of Presentation: Kyoto
  • Year and Date: 2011-07-26
• [Presentation] 敗血症患者における血中ADAMTS-13とvon Willebrand Factor Propeptideの変動 (2011)
  • Author(s): 福島英賢、西尾健治、高野啓佑、渡邉知朗、伊藤真吾、関匡彦、瓜園泰之、畑倫明、奥地一夫
  • Organizer: 第38回日本集中治療医学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2011-02-24
• [Presentation] (2011)
  • Author(s): 福島英賢
  • Organizer: 第38回日本集中治療医学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2011-02-24
• [Presentation] 敗血症患者における血中von Willebrand Factor Propeptideの変動 (2010)
  • Author(s): 福島英賢、浅井英樹、矢田憲孝、渡邉知朗、伊藤真吾、関匡彦、瓜園泰之、西尾健治、奥地一夫
  • Organizer: 37回日本集中治療医学会学術集会
  • Place of Presentation: 広島
  • Year and Date: 2010-03-04
• [Presentation] 敗血症患者における血中von Willebrand Factor Propeptideの変動 (2010)
  • Author(s): 福島英賢
  • Organizer: 日本集中治療学会
  • Place of Presentation: 広島
  • Year and Date: 2010-03-04