| Project/Area Number |
21791780
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
HASEBE Akira Hokkaido University, 大学院・歯学研究科, 助教 (90281815)
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| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | 口腔細菌学 / リポタンパク質 / 付着因子 / TLR2 / FSL-1 / クラスリン依存的 / CD14 / CD36 |
|---|
| Research Abstract |
To study the roles of bacterial lipoproteins on bacterial invasion to host cells and on the effects on intracellular trafficking in host cells, attempts were made to purify a mycoplasmal lipoprotein, since some mycoplasmas are known to be able to invade into host cells. In this study, a lipoprotein was purified from M. hominis. The 40 kDa-lipoprotein was purified, and its N-terminal amino acid sequence was identical to that of P50 lipoprotein, which had already been reported as an adhesin of M. hominis. Interestingly, this lipoprotein could adhere to THP-1 cells but anti TLR2 antibody failed to inhibit adherence of this lipoprotein to THP-1 cells. We are now under preparation for submitting these results to an international journal on immunology.
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