cytological evaluation on different effect of parathyroid hormone (PTH) in osteoblasts and preosteoblasts

• Project/Area Number: 21791784
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphological basic dentistry
• Research Institution: Hokkaido University
• Principal Investigator: LI Minqi Hokkaido University, 大学院・歯学研究科, 助教 (60447612)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 解剖学 / 細胞・組織 / 遺伝子 / 歯学 / 骨

Research Abstract

PTH administration led to significant increases in the pre-osteoblastic proliferation in both the wild-type and c-fos^<-/-> mice. The pre-osteoblasts present on the bone surfaces did not seem to be actively producing bone matrix in c-fos^<-/-> mice. We concluded that the absence of osteoclasts might hinder PTH-driven bone anabolism and that osteoclastic presence may be necessary for full osteoblastic differentiation and enhanced bone formation after the intermittent PTH administration.

Research Products

• [Journal Article] FGFR3 down-regulates PTH/PTHrP receptor gene expression by mediating JAK/STAT signaling in chondrocytic cell line. (2010)
  • Author(s): Li M, Seki Y, Freitas HLP, Nagata M, Kojima T, Sultana S, Ubaidus S, Maeda T, Shimomura-Kuroki J, Henderson J, Tamura M, Liu Z, Guo Y, Yamamoto T, Oda K, Takagi R, Amizuka N.
  • Journal Title: J Electron Microsc. 59(3) Pages: 227-236
  • NAID: 120002834687
  • Peer Reviewed
• [Journal Article] Ultrastructural observation on cells with characteristics of histological criteria for preosteoblasts in mouse tibial metaphysis. (2010)
  • Author(s): Narimatsu K, Li M, FreitaS PHL, Sultana S, Ubaidus S, Kojima T, Liu Z, Guo Y, Suzuki R Yamamoto T, Oda K, Amizuka N.
  • Journal Title: J Electron Microsc. 59(5) Pages: 427-436
  • Peer Reviewed
• [Journal Article] Immunolocalization of DMP-1 and sclerostin in the epiphyseal trabecule and diaphyseal cortical bone of osteoprotegerin deficient mice. (2010)
  • Author(s): Masuki H, Li M, Guo Y, Hasegawa T, Liu Z, Suzuki R, Oda K, Yamamoto T, Kawanami M, Amizuka N
  • Journal Title: Biomedical Res. 31(5) Pages: 307-318
  • NAID: 130004903810
  • Peer Reviewed
• [Journal Article] Ultrastructural Assessment of Mineral Crystallization and Collagen Mineralization in Bone. (2010)
  • Author(s): Li M, Hasegawa T, Masuki H, Liu Z, Guo Y, Suzuki R, Yamamoto T, Freitas PHL, Amizuka N
  • Journal Title: J.Oral Biosci. 52(2) Pages: 94-99
  • NAID: 10027105502
  • Peer Reviewed
• [Journal Article] Immunolocalization of DMP1 and sclerostin in the epiphyseal trabecule and diaphyseal cortical bone of osteoprotegerin deficient mice. (2010)
  • Author(s): Masuki H., Li M., et al.
  • Journal Title: Biomed Res. Volume: 31 Pages: 307-318
  • NAID: 130004903810
  • Peer Reviewed
• [Journal Article] Ultrastructural observation on cells meeting the histological criteria for preosteoblasts-a study in the mouse tibial metaphysis. (2010)
  • Author(s): Narimatsu K., Li M., et al.
  • Journal Title: J Electron Microsc (Tokyo) Volume: 59 Pages: 427-436
  • Peer Reviewed
• [Journal Article] Histological review of the human cellular cementum with special reference to an alternating lamellar pattern. (2010)
  • Author(s): Yamamoto T., Li M., et al.
  • Journal Title: Odontology Volume: 98 Pages: 102-109
  • NAID: 10029302597
  • Peer Reviewed
• [Journal Article] FGFR3 down-regulates PTH/PTHrP receptor gene expression by mediating JAK/STAT signaling in chondrocytic cell line (2010)
  • Author(s): Li, M., et al.
  • Journal Title: J Electron Microsc. (in press)
  • NAID: 120002834687
  • Peer Reviewed
• [Journal Article] 骨・軟骨に対するPTH/PTHrP作用における形態学的知見Clin Calcium (2009)
  • Author(s): 網塚憲生、李敏啓、田村正人、織田公光
  • Journal Title: 医薬ジャーネル社 19 Pages: 935-943
• [Journal Article] Intermittent PTH administration stimulates pre-osteoblastic proliferation without leading to enhanced bone formation in osteoclast-less c-fos (-/-) mice. (2009)
  • Author(s): Freitas PHL, Li M, Ninomiya T, Nakamura M, Ubaidus S, Oda K, Udagawa N, Maeda T, Takagi R, Amizuka N
  • Journal Title: J Bone Miner. Res. 24(9) Pages: 1586-1597
  • Peer Reviewed
• [Journal Article] FGF23 is mainly synthesized by osteocytes in the regularly distributed osteocytic lacunar canalicular system established after physiological bone remodeling. (2009)
  • Author(s): Ubaidus S, Li M, Sultana S, de Freitas PH, Oda K, Maeda T, Takagi R, Amizuka N.
  • Journal Title: J Electron Microsc. 58(6) Pages: 381-392
  • Peer Reviewed
• [Journal Article] Intermittent PTH administration stimulates pre-osteoblastic proliferation without leading to enhanced bone formation in osteoclast-less c-fos(-/-)mice (2009)
  • Author(s): Luiz de Freitas, PH., Li, M., et al.
  • Journal Title: J Bone Miner Res. 24 Pages: 1586-1597
  • Peer Reviewed
• [Journal Article] FGF23 is mainly synthesized by osteocytes in the regularly distributed osteocytic lacunar canalicular system established after physiological bone remodeling (2009)
  • Author(s): Ubaidus, S., Li, M., et al.
  • Journal Title: J Electron Microsc. 58 Pages: 381-392
  • Peer Reviewed
• [Presentation] Histochemical assessment of altered bone tissue in transgenic mice overexpressing parathyroid hormone-related peptide (PTHrP) driven by type I collagen promoter. (2010)
  • Author(s): Li M, Hasegawa T, Nakamura S, Sasaki M, Liu Z, Guo Y, Suzuki R, Yamamoto T, Amizuka N
  • Organizer: The American Society for Bone and Mineral Research (ASBMR), Metro Toronto Convention Centre
  • Place of Presentation: South Building Toronto, ON, Canada
  • Year and Date: 2010-10-17
• [Presentation] Histochemical assessment for altered bone tissue in transgenic mice over-expressing parathyroid hormone-related peptide (PTHrP) driven by type I collagen promoter (2010)
  • Author(s): Li M., et al.
  • Organizer: The 32^<nd> Annual Meeting of American Society for Bone and Mineral Research
  • Place of Presentation: Canada, Toronto
  • Year and Date: 2010-10-17
• [Presentation] 骨芽細胞特異的PTHrPトランジェニックマウスの骨組織における解析 (2010)
  • Author(s): 李敏啓、長谷川智香、柳鋳晟、増木英郎、佐々木宗輝、田幡千尋、小守壽文、山本恒之、網塚憲生
  • Organizer: 第52回歯科基礎医学会
  • Place of Presentation: タワーホール船堀、東京
  • Year and Date: 2010-09-21
• [Presentation] 骨芽細胞特異的PTHrP過剰発現マウスの骨組織における組織化学的解析 (2010)
  • Author(s): 李敏啓、郭頴、小守壽文、網塚憲生
  • Organizer: 第28回日本骨代謝学会
  • Place of Presentation: 京王プラザホテル、東京
  • Year and Date: 2010-07-23
• [Presentation] type I collagen promoterを用いたPTHrP Tgマウスの骨芽細胞における解析 (2010)
  • Author(s): 李敏啓、長谷川智香、柳鋳晟、郭穎、鈴木礼子、沢村祥子、山本恒之、網塚憲生
  • Organizer: 第30回日本骨形態計測学会
  • Place of Presentation: 米子コンベンションセンター、鳥取
  • Year and Date: 2010-05-14
• [Presentation] Histological assessment for PTH/PTHrP signaling on osteoblasts. (2010)
  • Author(s): Amizuka N, Li M, Freitas PHL, Liu Z, Guo Y, Suzuki R, Yamamoto T
  • Organizer: 14th international congress of endocrinology
  • Place of Presentation: Kyoto International Conference Center, Kyoto, JAPAN
  • Year and Date: 2010-03-28
• [Presentation] type I collagen promoterを用いたPTHrP Tgマウスの骨芽細胞における解析 (2010)
  • Author(s): 網塚憲生、長谷川智香、中村沙也香、Paulo Freitas、柳鋳晟、郭頴、鈴木礼子、李敏啓
  • Organizer: 第22回北海道骨粗髪症研究会
  • Place of Presentation: 北海道大学医学部臨床講義棟大講堂、札幌
  • Year and Date: 2010-01-23
• [Presentation] 副甲状腺ホルモン問歓投与による前骨芽細胞の分化増殖について (2010)
  • Author(s): 網塚憲生、長谷川智香、Paulo Freitas、柳鋳晟、郭頴、鈴木礼子、李敏啓
  • Organizer: 第22回北海道骨粗鬆症研究会
  • Place of Presentation: 北海道大学医学部臨床講義棟大講堂、札幌
  • Year and Date: 2010-01-23
• [Presentation] アスコルビン酸合成能欠如ラットの骨基質石灰化における微細構造学的観察 (2009)
  • Author(s): 李敏啓, など
  • Organizer: 日本解剖学会第55回東北・北海道連合支部学術集会
  • Place of Presentation: 東北大学医学部, 仙台
  • Year and Date: 2009-09-26
• [Remarks] ホームページ
  • URL: http://www.den.hokudai.ac.jp/anatomy2/hokudai_d/index.html