Searching for environmental signals sensed by group A streptococcal two-component systems

• Project/Area Number: 21791786
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphological basic dentistry
• Research Institution: Osaka University
• Principal Investigator: NAKATA Masanobu Osaka University, 大学院・歯学研究科, 助教 (90444497)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 二成分制御系 / 病原因子 / 環境応答 / 転写調節 / レンサ球菌

Research Abstract

Streptococcus pyogenes (group A streptococci; GAS) is a human pathogen responsible for a wide variety of diseases that ranges from self-limiting purulent infections such as pharyngitis, tonsillitis, and impetigo to severe necrotizing fasciitis and autoimmune diseases including acute rheumatic fever and glomerulonephritis. To successfully evade host immune responses and cause such diverse diseases, GAS might acclimate to environmental changes and control the expression of a variety of genes including virulence genes. In this study, deletion mutants of genes encoding both histidine kinases and response regulators were constructed in a background of a serotype M3 GAS strain. Utilizing these mutant strains and a silkworm infection model, we revealed that several two-component systems are crucial for GAS pathogenesis. Moreover, analysis of the virulence gene expression in mutant strains under several culture conditions indicated specific environmental signals are sensed by two-component systems.

Research Products

• [Journal Article] Pili of oral Streptococcus sanguinis bind to salivary amylase and promote the biofilm formation. (2011)
  • Author(s): Okahashi N, Nakata M, Terao Y, Isoda R, Sakurai A, Sumitomo T, Yamaguchi M, Kimura RK, Oiki E, Kawabata S, Ooshima T.
  • Journal Title: Microb Pathog 50(3-4) Pages: 148-154
  • Peer Reviewed
• [Journal Article] Streptolysin S contributes to group A streptococcal translocation across an epithelial barrier. (2011)
  • Author(s): Sumitomo T, Nakata M, Higashino M, Jin Y, Terao Y, Fujinaga Y, Kawabata S.
  • Journal Title: J Biol Chem 286(4) Pages: 2750-2761
  • Peer Reviewed
• [Journal Article] Streptolysin S contributes to group A streptococcal translocation across an epithelial barrier (2011)
  • Author(s): Sumitomo, I., Nakata, M., Higashino, M., Jin, Y., Terao, Y., Fujinaga, Y., Kawabata, S.
  • Journal Title: Journal of Biological Chemistry Volume: 286 Pages: 2750-2761
  • Peer Reviewed
• [Journal Article] Pili of oral Streptococcus sanguinis bind to salivary amylase and promote the biofilm formation (2011)
  • Author(s): Okahashi, N., Nakata, M., Terao, Y., Isoda, R., Sakurai, A., Sumitomo, T., Yamaguchi, M., Kimura, R.K., Oiki, E., Kawabata, S., Ooshima, T.
  • Journal Title: Microbial Pathogenesis Volume: 50 Pages: 148-154
  • Peer Reviewed
• [Journal Article] Pili of oral Streptococcus sanguinis bind to fibronectin and contribute to cell adhesion. (2010)
  • Author(s): Okahashi N, Nakata M, Sakurai A, Terao Y, Hoshino T, Yamaguchi M, Isoda R, Sumitomo T, Nakano K, Kawabata S, Ooshima T.
  • Journal Title: Biochem Biophys Res Commun 391(2) Pages: 1192-1196
  • Peer Reviewed
• [Journal Article] Mode of expression and functional characterization of FCT-3 pilus region encoded proteins in the Streptococcus pyogenes serotype M49. (2009)
  • Author(s): Nakata M, Koller T, Moritz K, Ribardo D, Jonas L, McIver KS, Sumitomo T, Terao Y, Kawabata S, Podbielski A, Kreikemeyer B.
  • Journal Title: Infect Immun 77(1) Pages: 32-44
  • Peer Reviewed
• [Journal Article] M-protein and other intrinsic virulence factors of Streptococcus pyogenes are encoded on an ancient pathogenicity island. (2009)
  • Author(s): Panchaud A, Guy L, Collyn F, Haenni M, Nakata M, Podbielski A, Moreillon P, Roten CA.
  • Journal Title: BMC Genomics 10 Pages: 198-198
  • Peer Reviewed
• [Journal Article] M-protein and other intrinsic virulence factors of Streptococcus pyogenes are encoded on an ancient pathopenicity island. (2009)
  • Author(s): Panchaud, A.
  • Journal Title: BMC Genomics 10 Pages: 198-198
  • Peer Reviewed
• [Journal Article] Pili of oral Streptococcus sanguinis bind to fibroncctin and contribute to cell adhesion. (2009)
  • Author(s): Okahashi, N.
  • Journal Title: Biochemical and Biophysical Research Communications 391 Pages: 1192-1196
  • Peer Reviewed
• [Journal Article] Mode of expression and functional characterization of FCT-3 pilus region encoded proteins in the Streptococcus pyogenes serotype M49. (2009)
  • Author(s): Nakata, M.
  • Journal Title: Infection and Immunity 77 Pages: 32-34
  • Peer Reviewed
• [Presentation] M6型A群レンサ球菌が産生する線毛の形成機構と機能の解析 (2010)
  • Author(s): 木村敬次リチャード, 中田匡宣, 住友倫子, 寺尾豊, 磯田竜太朗, 川端重忠
  • Organizer: 第63回日本細菌学会関西支部総会
  • Place of Presentation: 関西医科大学
  • Year and Date: 2010-11-20
• [Presentation] M6型A群レンサ球菌が産生する線毛の形成機構と機能の解析 (2010)
  • Author(s): 木村敬次リチャード, 中田匡宣, 住友倫子, 寺尾豊, 磯田竜太朗, 川端重忠
  • Organizer: 第63回日本細菌学会関西支部総会
  • Place of Presentation: 関西医科大学(大阪府)
  • Year and Date: 2010-11-20
• [Presentation] A群レンサ球菌の上皮バリア突破機構に関与する宿主プロテアーゼの検索 (2010)
  • Author(s): 住友倫子, 中田匡宣, 東野美晴, 寺尾豊, 川端重忠
  • Organizer: 第8回感染症沖縄フォーラム
  • Place of Presentation: 沖縄国民年金健康センター
  • Year and Date: 2010-02-12
• [Presentation] Streptolysin S contributes to group A streptococcal paracellular translocation across epithelial cells. (2010)
  • Author(s): Sumitomo T, Nakata M, Terao Y, Kawabata S.
  • Organizer: The 10th Awaji International Forum on Infection and Immunity.
  • Place of Presentation: Awaji, Hyogo, Japan.
• [Presentation] Streptolysin S contributes to group A streptococcal paracellular translocation across epithelial cells. (2010)
  • Author(s): 住友倫子, 中田匡宣, 寺尾豊, 川端重忠
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場
• [Presentation] 口腔レンサ球菌S.sanguinis線毛piliの機能 (2010)
  • Author(s): 岡橋暢夫, 中田匡宣, 桜井敦朗, 寺尾豊, 山口雅也, 星野倫範, 川端重忠, 大嶋隆
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 宿主細胞間接着分子の破壊に関与するA群レンサ球菌プロテアーゼの検索 (2010)
  • Author(s): 東野美晴, 住友倫子, 中田匡宣, 寺尾豊, 川端重忠
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] A群レンサ球菌の上皮バリア突破機構に関与する宿主プロテアーゼの解析 (2010)
  • Author(s): 住友倫子, 中田匡宣, 東野美晴, 寺尾豊, 川端重忠
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] A群レンサ球菌が産生する線毛の細胞壁架橋機構の解析 (2010)
  • Author(s): 中田匡宣, 住友倫子, 磯田竜太朗, 寺尾豊, 川端重忠
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Streptolysin S contributes to group A streptococcal paracellular translocation across epithelial cells. (2010)
  • Author(s): 住友倫子, 中田匡宣, 寺尾豊, 川端重忠
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場(大阪市)
• [Presentation] Streptolysin S contributes to group A streptococcal paracellular translocation across epithelial cells. (2010)
  • Author(s): Sumitomo, T., Nakata, M., Terao, Y., Kawabata, S.
  • Organizer: The 10th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 淡路夢舞台国際会議場(兵庫県)
• [Presentation] A群レンサ球菌が産生する線毛の細胞壁架橋機構の解析 (2010)
  • Author(s): 中田匡宣
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜(横浜市)
• [Presentation] A群レンサ球菌の上皮バリア突破機構に関与する宿主プロテアーゼの解析 (2010)
  • Author(s): 住友倫子
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜(横浜市)
• [Presentation] 宿主細胞間接着分子の破壊に関与するA群レンサ球菌プロテアーゼの検索 (2010)
  • Author(s): 東野美晴
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜(横浜市)
• [Presentation] 口腔レンサ球菌S. Sanguinis線毛piliの機能 (2010)
  • Author(s): 岡橋暢夫
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜(横浜市)
• [Presentation] A群レンサ球菌が産生する線毛の発現機構の解析 (2009)
  • Author(s): 中田匡宣, 寺尾豊, 川端重忠
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 朱鷺メッセ,新潟市
• [Presentation] Group A Streptococci translocates across epithelial barrier via intercellular junction cleavage. (2009)
  • Author(s): Sumitomo T, Nakata M, Terao Y, Kawabata S.
  • Organizer: 109th General Meeting of American Society for Microbiology.
  • Place of Presentation: Philadelphia, USA.
• [Presentation] Group A Streptococci translocates across epithelial barrier via intercellular junction eleavage. (2009)
  • Author(s): Sumitomo, T.
  • Organizer: 109th General Meeting of American Society for Microbiology
  • Place of Presentation: Philadelphia, USA
• [Presentation] A群レンサ球菌が産生する線毛の発現機構の解析 (2009)
  • Author(s): 中田匡宣
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 朱鷺メッセ(新潟市)
• [Presentation] A群レンサ球菌の上皮バリア突破機構に関与する宿主プロテアーゼの検索 (2009)
  • Author(s): 住友倫子
  • Organizer: 第7回感染症沖縄フォーラム
  • Place of Presentation: 沖縄国民年金健康センター(那覇市)
• [Book] 口腔内微生物の網羅的検出.歯界展望.116(4) (2010)
  • Author(s): 中田匡宣, 川端重忠
  • Publisher: 医歯薬出版
• [Remarks] ホームページ等
  • URL: http://www.dent.osaka-u.ac.jp/~mcrbio
• [Remarks]
  • URL: http://www.dent.osaka-u.ac.jp/~mcrbio