The roles of Wnt/β-catenin signaling on differentiation of mesenchymal progenitor cells.

• Project/Area Number: 21791799
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphological basic dentistry
• Research Institution: Nihon University
• Principal Investigator: NAITO Masako 日本大学, 歯学部, 助教 (40436803)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 間葉系前駆細胞 / 脂肪細胞 / 骨芽細胞 / 分化 / 間葉系幹細胞 / 脂肪細胞分化 / 骨芽細胞分化 / Wnt/β-catenin / Wnt / β-catenin

Research Abstract

In this study, we examined the expression and activity of Wnt/β-catenin signaling pathway molecules in mesenchymal progenitor cells(ROB-C26). Immunocytochemical analysis and gene-silencing experiments revealed that the inhibition of β-catenin expression by Dex promotes the differentiation of mesenchymal progenitor cells into adipocytes.

Research Products

• [Journal Article] Suppression of lamin A/C by short hairpin RNAs promotes adipocyte lineage commitment in mesenchymal progenitor cell line, ROB-C26 (2012)
  • Author(s): Naito M, Omoteyama K, Mikami Y, Takagi M, Takahashi T.
  • Journal Title: Histochem Cell Biol Volume: 137 Pages: 235-247
  • Peer Reviewed
• [Journal Article] Real-time in vivo imaging of p16Ink4a reveals cross talk with p53. (2009)
  • Author(s): Yamakoshi K, et. al.
  • Journal Title: J Cell Biol. 186 Pages: 393-407
  • Peer Reviewed
• [Journal Article] Identification of tumor-initiating cells in a highly aggressive brain tumor using Promoter activity of nucleostemin. (2009)
  • Author(s): Tamase A, et. al.
  • Journal Title: Proc Natl Acad Sci USA. 106 Pages: 17163-17168
  • Peer Reviewed
• [Presentation] 脂肪細胞分化におけるlamin A/Cの役割 (2012)
  • Author(s): 内藤昌子、三上剛和、高橋富久
  • Organizer: 第117回日本解剖学会
  • Place of Presentation: 山梨大学甲府キャンパス、山梨県
  • Year and Date: 2012-03-27
• [Presentation] 脂肪細胞分化におけるlamin A/Cの役割 (2012)
  • Author(s): 内藤昌子
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨大学甲府キャンパス(山梨県)
  • Year and Date: 2012-03-27
• [Presentation] 脂肪細胞分化におけるWnt/β-cateninシグナルの役割 (2011)
  • Author(s): 内藤昌子、高橋富久
  • Organizer: 第99回日本解剖学会関東支部会
  • Place of Presentation: 日本大学松戸歯学部、千葉県
  • Year and Date: 2011-10-15
• [Presentation] 脂肪細胞分化におけるWnt/β-cateninシグナルの役割 (2011)
  • Author(s): 内藤昌子
  • Organizer: 第52回日本解剖学会関東支部会
  • Place of Presentation: 日本大学松戸歯学部(千葉県)
  • Year and Date: 2011-10-15
• [Presentation] Dexamethasone(Dex)誘導性脂肪細胞分化におけるWnt/β-cateninシグナルの役割 (2010)
  • Author(s): 内藤昌子、高城稔
  • Organizer: 第52回歯科基礎医学会
  • Place of Presentation: タワーホール船堀、東京都
  • Year and Date: 2010-09-22
• [Presentation] Dexamethasone (Dex)誘導性脂肪細胞分化におけるWnt/β-cateninシグナルの役割 (2010)
  • Author(s): 内藤昌子
  • Organizer: 第52回歯科基礎医学会
  • Place of Presentation: タワーホール船堀(東京都)
  • Year and Date: 2010-09-22