Relationship between transcriptional activity and structure of tooth specific transcription factor Sp6 and identification of its interaction molecules

• Project/Area Number: 21791805
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional basic dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: MIYOSHI Keiko 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 講師 (20304537)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 転写因子 / SP6 / 機能ドメイン / 転写活性 / 細胞内局在 / 標的配列 / 活性-構造相関 / 相互作用分子 / Sp6 / 核移行シグナル

Research Abstract

Through the generation of SP6 inducible system, we found that SP6 is the short lived protein and degrades via ubiquitin-independent proteasome system. We further established inducible SP6 expression system and identified some candidate genes of SP6 target by microarray analysis. Furthermore, it is suggested that the full length of SP6 and its structure may be important to regulate the subcellular localization and transcriptional activity of SP6.

Research Products

• [Journal Article] Dissection of morphological and metabolic differentiation of ameloblasts via ectopic SP6 expression (2012)
  • Author(s): Muto T, Miyoshi K, Horiguchi T, Noma T
  • Journal Title: J Med Invest Volume: 59 Pages: 59-68
  • NAID: 130004465272
  • Peer Reviewed
• [Journal Article] Possible linkage of SP6 transcriptional activity with amelogenesis by protein stabilization (2011)
  • Author(s): Utami TW, Miyoshi K, Hagita H, Yanuaryska RD, Horiguchi T, Noma T
  • Journal Title: J Biomed Biotech Volume: ID320987 Issue: 1 Pages: 1-10
  • DOI: 10.1155/2011/320987
  • NAID: 120006783225
  • Peer Reviewed
• [Journal Article] Dynamic changes of Sp6 transgene expression in dental epithelial cells during long-term culture (2011)
  • Author(s): Utami TW, et al
  • Journal Title: Indones J Dent Res Volume: (in press)
  • Peer Reviewed
• [Journal Article] Possible linkage of SP6 transcriptional activity with amelogenesis by protein stabilization (2011)
  • Author(s): Utami TW, et al
  • Journal Title: J Biomed Biotechnol Pages: 1-10
  • Peer Reviewed
• [Journal Article] Isolation and characterization of mouse Specificity Protein 6 promoter (2010)
  • Author(s): Wahyudi IA, Horiguchi T, Miyoshi K, Muto T, Utami TW, Hagita H, Noma T
  • Journal Title: Indones J Dent Res Volume: 1(1) Pages: 21-34
  • NAID: 120006783224
  • Peer Reviewed
• [Journal Article] Isolation and Characterization of Mouse Specificity Protein 6 Promoter (2010)
  • Author(s): Ivan Arie Wahyudi
  • Journal Title: Indonesian J.Dent.Res. Volume: 1 Pages: 21-34
  • Peer Reviewed
• [Journal Article] Novel genetic linkage of rat Sp6 mutation to amelogenesis imperfecta
  • Author(s): Muto T*, Miyoshi K*, Hagita H, Horiguchi T, Noma T
  • Journal Title: Orphanet J Rare Dis Volume: (in press)
  • NAID: 120006783222
  • Peer Reviewed
• [Journal Article] Dynamic Changes of Sp6 Transgene Expression in Dental Epithelial Cells during Long-term Culture
  • Author(s): Utami TW, Miyoshi K, Hagita H, Yanuaryska RD, Horiguchi T, Noma T
  • Journal Title: Indones J Dent Res Volume: (in press)
  • NAID: 120006783223
  • Peer Reviewed
• [Presentation] SP6 stability and functional linkage during amelogenesis (2011)
  • Author(s): Utami TW, et al
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 長良川国際会議場(岐阜県)
  • Year and Date: 2011-10-01
• [Presentation] Identification of SP6 target genes in rat dental epithelial cells (2011)
  • Author(s): Yanuaryska RD
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Place of Presentation: 長良川国際会議場(岐阜県)
  • Year and Date: 2011-10-01
• [Presentation] SP6 stability and functional linkage during amelogenesis (2011)
  • Author(s): Utami TW, et al
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 第84回日本生化学会大会
  • Year and Date: 2011-09-22
• [Presentation] SP6 stability and functional linkage during amelogenesis (2011)
  • Author(s): Utami TW, et al
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2011-09-22
• [Presentation] Regulation of Sp6 expression and function in dental epithelial cells (2011)
  • Author(s): Utami TW, et al
  • Organizer: 第5回日本エピジェネティクス研究会年会
  • Place of Presentation: KKRホテル熊本(熊本県)
  • Year and Date: 2011-05-20
• [Presentation] Regulation of Sp6 expression and function in C9 cells (2011)
  • Author(s): Utami TW, et al
  • Organizer: 第52回日本生化学会中国・四国支部例会
  • Place of Presentation: 広島大学広仁会館(広島県)
  • Year and Date: 2011-05-13
• [Presentation] Differential regulation of Sp6 silencing in dental epithelial cells (2010)
  • Author(s): Utami TW, et al
  • Organizer: International Joint Symposium on Oral Science
  • Place of Presentation: Kuta Bali, Indonesia
  • Year and Date: 2010-12-17
• [Presentation] Regulation of Sp6 Expression in CHA9 Cells (2010)
  • Author(s): Utami TW, et al
  • Organizer: 33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸国際展示場(神戸市)
  • Year and Date: 2010-12-08
• [Presentation] Regulation of Sp6 Expression in CHA9 Cells (2010)
  • Author(s): Trianna Wahyu Utami
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08