Reception, transduction, and modulation of sweet taste in taste receptor cells : Analysis by using transgenic mice.

• Project/Area Number: 21791808
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional basic dentistry
• Research Institution: Kyushu University
• Principal Investigator: YOSHIDA Ryusuke Kyushu University, 大学院・歯学研究院, 助教 (60380705)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 味覚 / 味細胞 / 甘味 / 遺伝子改変マウス / 受容体 / 情報伝達機構 / カンナビノイド / 甘味受容体 / 情報伝逹機構 / レプチン

Research Abstract

This study has revealed (1) Endocannabinoids selectively enhance sweet taste via CB1 receptor on sweet sensitive taste cells, (2) Taste cells expressing T1R3, a sweet and umami taste receptor component, that are identified by using T1R3-GFP mice respond to various sweet and/or umami taste stimuli, (3) T1R3-GFP taste cells lacking T1R3, gustducin, or TRPM5 do not respond to taste stimuli, (4) T1R3-GFP taste cells express Gα11, 14, s, q, and i2 as Gα subunits other than gustducin.

Research Products

• [Journal Article] Reception and transmission of taste information in Type II and Type III taste bud cells. (2010)
  • Author(s): Yoshida R, Niki M, Murata Y, Shigemura N, Ninomiya Y
  • Journal Title: J.Oral.Biosci. 52 Pages: 358-364
  • NAID: 10027625952
• [Journal Article] Endocannabinoids selectively enhance sweet taste. (2010)
  • Author(s): Yoshida R, Ohkuri T, Jyotaki M, Yasuo T, Horio N, Yasumatsu K, Sanematsu K, Shigemura N, Yamamoto T, Margolskee RF, Ninomiya Y
  • Journal Title: Proc.Natl.Acad.Sci.U S A 107 Pages: 935-939
• [Journal Article] New Insights into the Signal Transmission from Taste Cells to Gustatory Nerve Fibers. (2010)
  • Author(s): Yoshida R, Ninomiya Y
  • Journal Title: Int.J.Cell.Mol.Biol. 279 Pages: 102-134
• [Journal Article] 内因性カンナビノイドの甘味増強作用 (2010)
  • Author(s): 吉田竜介、大栗弾宏、重村憲徳、二ノ宮裕三
  • Journal Title: 実験医学 28 Pages: 1405-1408
• [Journal Article] 末梢における味覚受容・情報伝達とその調節 (2010)
  • Author(s): 吉田竜介、仁木麻由、二ノ宮裕三
  • Journal Title: Clinical Neuroscience 28 Pages: 1227-1231
• [Journal Article] Reception and transmission of taste information in Type II and Type III taste bud cells. (2010)
  • Author(s): Yoshida R, Niki M, Murata Y, Shigemura N, Ninomiya Y
  • Journal Title: J.Oral.Biosci. Volume: 52 Pages: 358-364
  • NAID: 10027625952
  • Peer Reviewed
• [Journal Article] Gustatory Signaling in the periphery: Detection, Transmission, and Modulation of Taste Information. (2010)
  • Author(s): Niki M, Yoshida R, Takai S, Ninomiya Y
  • Journal Title: Biol.Pharm.Bull. Volume: 33 Pages: 1772-1777
  • NAID: 130000402293
  • Peer Reviewed
• [Journal Article] Reciprocal modulation of sweet taste by leptin and endocannabinoids. (2010)
  • Author(s): Niki M, Jyotaki M, Yoshida R.Ninomiya Y
  • Journal Title: Results Probl.Cell Differ. Volume: 52 Pages: 101-114
  • Peer Reviewed
• [Journal Article] Action potential-enhanced ATP release from taste cells through hemichannels. (2010)
  • Author(s): Murata Y, Yasuo T, Yoshida R, Obato K, Yanagawa Y, Margolskee RF, Ninomiya Y
  • Journal Title: J.Neurophysiol. Volume: 104 Pages: 896-901
  • Peer Reviewed
• [Journal Article] Taste preference for fatty acids is mediated by GPR40 and GPR120. (2010)
  • Author(s): Cartoni C, Yasumatsu K, Ohkuri T, Shigemura N, Yoshida R, Godinot N, le Coutre J, Ninomiya Y, Damak S
  • Journal Title: J.Neurosci. Volume: 30 Pages: 8376-8382
  • Peer Reviewed
• [Journal Article] 内因性カンナビノイドの甘味増強作用 (2010)
  • Author(s): 吉田竜介、大栗弾宏、重村憲徳、二ノ宮裕三
  • Journal Title: 実験医学 Volume: 28 Pages: 1405-1408
• [Journal Article] 末梢における味覚受容・情報伝達とその調節 (2010)
  • Author(s): 吉田竜介、仁木麻由、二ノ宮裕三
  • Journal Title: Clinical Neuroscience Volume: 28 Pages: 1227-1231
• [Journal Article] マウス味細胞の味応答時におけるGABAの作用 (2010)
  • Author(s): 安尾敏明、吉田竜介、柳川右千夫、Margolskee RF、二ノ宮裕三
  • Journal Title: 日本味と匂学会誌 Volume: 17 Pages: 227-230
  • NAID: 110008451321
  • Peer Reviewed
• [Journal Article] エンドカンナビノイド受容体アンタゴニストによるdb/dbマウスにおける甘味応答抑制 (2010)
  • Author(s): 仁木麻由、大栗弾宏、上瀧将史、吉田竜介、二ノ宮裕三
  • Journal Title: 日本味と匂学会誌 Volume: 17 Pages: 231-234
  • NAID: 110008451322
  • Peer Reviewed
• [Journal Article] Endocannabinoids selectively enhance sweet taste. (2010)
  • Author(s): Yoshida R, Ohkuri T, Jyotaki M, Yasuo T, Horio N, Yasumatsu K, Sanematsu K, Shigemura N, Yamamoto T, Margolskee RF, Ninomiya Y
  • Journal Title: Proc.Natl.Acad.Sci. U S A 107 Pages: 935-939
  • Peer Reviewed
• [Journal Article] New Insights into the Signal Transmission from Taste Cells to Gustatory Nerve Fibers (2010)
  • Author(s): Yoshida R, Ninomiya Y
  • Journal Title: Int.J.Cell.Mol.Biol. 279 Pages: 102-134
• [Journal Article] New frontiers in gut nutrient sensor research : nutrient sensors in the gastrointestinal tract : modulation of sweet taste sensitivity by leptin. (2010)
  • Author(s): Horio N, Jyotaki M, Yoshida R, Sanematsu K, Shigemura N, Ninomiya Y
  • Journal Title: J.Pharmacol.Sci. 112 Pages: 8-12
  • NAID: 10027908348
  • Peer Reviewed
• [Journal Article] Discrimination of taste qualities among mouse fungiform taste bud cells. (2009)
  • Author(s): Yoshida R, Miyauchi A, Yasuo T, Jyotaki M, Murata Y, Yasumatsu K, Shigemura N, Yanagawa Y, Obata K, Ueno H, Margolskee RF, Ninomiya Y
  • Journal Title: J.Physiol. 587 Pages: 4425-4439
• [Journal Article] 味覚情報のコーディングにおける味細胞の役割 (2009)
  • Author(s): 吉田竜介、二ノ宮裕三
  • Journal Title: 味と匂学会誌 16 Pages: 117-124
• [Journal Article] Discrimination of taste qualities among mouse fungiform taste bud cells (2009)
  • Author(s): Yoshida R, Miyauchi A, Yasuo T, Jyotaki M, Murata Y, Yasumatsu K, Shigemura N, Yanagawa Y, Obata K, Ueno H, Margolskee RF, Ninomiya Y
  • Journal Title: J.Physiol. 587 Pages: 4425-4439
  • Peer Reviewed
• [Journal Article] Multiple receptors underlie glutamate taste responses in mice (2009)
  • Author(s): Yasumatsu K, Horio N, Murata Y, Shirosaki S, Ohkuri T, Yoshida R, Ninomiya Y
  • Journal Title: Am.J.Clin.Nutr. 90
  • Peer Reviewed
• [Journal Article] Variation in umami perception and in candidate genes for the umami receptor in mice and humans. (2009)
  • Author(s): Shigemura N, Shirosaki S, Ohkuri T, Sanematsu K, Islam AA, Ogiwara Y, Kawai M, Yoshida R, Ninomiya Y
  • Journal Title: Am.J.Clin.Nutr. 90
  • Peer Reviewed
• [Journal Article] Genetic and molecular basis of individual differences in human umami taste perception. (2009)
  • Author(s): Shigemura N, Shirosaki S, Sanematsu K, Yoshida R, Ninomiya Y
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] 味覚情報のコーディングにおける味細胞の役割 (2009)
  • Author(s): 吉田竜介,二ノ宮裕三
  • Journal Title: 日本味と匂学会誌 16 Pages: 117-124
  • Peer Reviewed
• [Journal Article] マウス味細胞におけるGABAの機能解析 (2009)
  • Author(s): 安尾敏明、吉田竜介、堀尾奈央、重村憲徳、二ノ宮裕三
  • Journal Title: 日本味と匂学会誌 16 Pages: 323-326
  • NAID: 110007568727
  • Peer Reviewed
• [Presentation] 味覚センサーの興奮性調節による行動変化 (2011)
  • Author(s): 吉田竜介、二ノ宮裕三
  • Organizer: 第88回日本生理学会大会
  • Place of Presentation: 横浜(震災により誌上に変更)
• [Presentation] マウス味細胞の味応答に対するGABAの影響 (2010)
  • Author(s): 安尾敏明、吉田竜介、重村憲徳、柳川右千夫、二ノ宮裕三
  • Organizer: 第52回歯科基礎医学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-21
• [Presentation] PKD2L1はマウス酸味受容に関与する (2010)
  • Author(s): 堀尾奈央、吉田竜介、安松啓子、二ノ宮裕三
  • Organizer: 第52回歯科基礎医学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-21
• [Presentation] db/dbマウスにおけるAM251の甘味応答抑制 (2010)
  • Author(s): 仁木麻由、大栗弾宏、上瀧将史、吉田竜介、二ノ宮裕三
  • Organizer: 第52回歯科基礎医学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-21
• [Presentation] マウス味細胞の味応答時におけるGABAの作用 (2010)
  • Author(s): 安尾敏明、吉田竜介、柳川右千夫、Margolskee RF、二ノ宮裕三
  • Organizer: 日本味と匂学会第44回大会
  • Place of Presentation: 北九州
  • Year and Date: 2010-09-09
• [Presentation] PKD2L1はマウス酸味応答に関与する (2010)
  • Author(s): 堀尾奈央、吉田竜介、安松啓子、柳川右千夫、石丸善朗、松波宏明、二ノ宮裕三
  • Organizer: 日本味と匂学会第44回大会
  • Place of Presentation: 北九州
  • Year and Date: 2010-09-09
• [Presentation] エンドカンナビノイド受容体アンタゴニストによるdb/dbマウスにおける甘味応答抑制 (2010)
  • Author(s): 仁木麻由、大栗弾宏、上瀧将史、吉田竜介、二ノ宮裕三
  • Organizer: 日本味と匂学会第44回大会
  • Place of Presentation: 北九州
  • Year and Date: 2010-09-09
• [Presentation] GABAのマウス味細胞応答に及ぼす影響 (2010)
  • Author(s): 安尾敏明、吉田竜介、重村憲徳、Margolskee RF、二ノ宮裕三
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-04
• [Presentation] PKD2L1は酸味応答に関与する (2010)
  • Author(s): 堀尾奈央、吉田竜介、安松啓子、柳川右千夫、石丸善朗、松波宏明、二ノ宮裕三
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-04
• [Presentation] 味細胞における味覚情報処理機構 (2010)
  • Author(s): 吉田竜介、二ノ宮裕三
  • Organizer: 平成22年電気学会 電子・情報・システム部門大会
  • Place of Presentation: 熊本
  • Year and Date: 2010-09-02
• [Presentation] PKD2L1 is required for normal chorda tympani nerve responses to acids. (2010)
  • Author(s): Horio N, Yoshida R, Ishimaru Y, Matsunami H, Ninomiya Y
  • Organizer: AChemS 2010
  • Place of Presentation: St.Pete Beach, FL, USA
  • Year and Date: 2010-04-23
• [Presentation] Expression of GABA receptor subunits and Cl- transporters of taste buds in mice (2010)
  • Author(s): Yasuo T, Yoshida R., Shigemura N, Margolskee RF, Ninomiya Y
  • Organizer: AChemS 2010
  • Place of Presentation: St.Pete Beach, FL, USA
  • Year and Date: 2010-04-23
• [Presentation] Endocannabinoids as modulators of sweet taste sensitivities in mice (2010)
  • Author(s): Yoshida R, Ohkuri T, Jyotaki M, Yasuo T, Horio N, Yasumatsu K, Sanematsu K, Shigemura N, Yamamoto T, Margolskee RF, Ninomiya Y
  • Organizer: International Symposium on "Oral Health Science" in 2010
  • Place of Presentation: 福岡
  • Year and Date: 2010-02-06
• [Presentation] マウス味細胞の生理機能解析 (2010)
  • Author(s): 吉田竜介
  • Organizer: 日本味と匂学会第44回大会
  • Place of Presentation: 北九州
• [Presentation] 内因性カンナビノイドはマウスの甘味応答を増大させる (2010)
  • Author(s): 吉田竜介、大栗弾宏、上瀧将史、安尾敏明、堀尾奈央、安松啓子、實松啓介、重村憲徳、山本経之、Margolskee RF、二ノ宮裕三
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸
• [Presentation] 味細胞における味覚情報処理機構 (2010)
  • Author(s): 吉田竜介、二ノ宮裕三
  • Organizer: 平成22年電気学会電子・情報・システム部門大会
  • Place of Presentation: 熊本
• [Presentation] カンナビノイドによるマウス甘味応答の増強 (2010)
  • Author(s): 吉田竜介、二ノ宮裕三
  • Organizer: 日本比較生理生化学会第32回大会
  • Place of Presentation: 福岡
• [Presentation] Endocannabinoids as modulators of sweet taste sensitivities in mice. (2010)
  • Author(s): Yoshida R, Ohkuri T, Jyotaki M, Yasuo T, Horio N, Yasumatsu K, Sanematsu K, Shigemura N, Yamamoto T, Margolskee RF, Ninomiya Y
  • Organizer: International Symposium on "Oral Health Science"
  • Place of Presentation: Fukuoka
• [Presentation] Taste responses of mouse fungiform taste bud cells expressing gustducin or GAD67 (2009)
  • Author(s): Yoshida R, Ohkuri T, Jyotaki M, Yasuo T, Horio N, Yasumatsu K, Sanematsu K, Shigemura N, Yamamoto T, Margolskee RF, Ninomiya Y
  • Organizer: The 7th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception
  • Place of Presentation: 福岡
  • Year and Date: 2009-11-04
• [Presentation] 味細胞における味覚の受容と情報伝達 (2009)
  • Author(s): 吉田竜介、村田芳博、安松啓子、重村憲徳、二ノ宮裕三
  • Organizer: 第51回歯科基礎医学会学術大会サテライトシンポジウム
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-09
• [Presentation] Taste responses of Type II and III taste bud cells in mouse fungiform papillae. (2009)
  • Author(s): Yoshida R, Yasuo T,Murata Y, Jyotaki M, Yanagawa Y, Obata K, Ueno H, Damak S, Margolskee RF, Ninomiya Y
  • Organizer: IUPS2009
  • Place of Presentation: 京都
  • Year and Date: 2009-07-27
• [Presentation] Different response properties between Type II and Type III taste bud cells in mouse fungiform papillae (2009)
  • Author(s): Yoshida R, Yasuo T, Murata Y, Jyotaki M, Yanagawa Y, Obata K, Ueno H, Margolskee RF, Ninomiya Y
  • Organizer: ACHEMS 2009, 31^<st> annual meeting
  • Place of Presentation: Sarasota, FL, USA
  • Year and Date: 2009-04-24
• [Presentation] Taste responses of mouse fungiform taste bud cells expressing gustducin or GAD67. (2009)
  • Author(s): Yoshida R, Ohkuri T, Jyotaki M, Yasuo T, Horio N, Yasumatsu K, Sanematsu K, Shigemura N, Yamamoto T, Margolskee RF, Ninomiya Y
  • Organizer: The 7th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception
  • Place of Presentation: Fukuoka
• [Presentation] マウス茸状乳頭味細胞の複数種の苦味、酸味物質に対する応答 (2009)
  • Author(s): 吉田竜介、安尾敏明、宮内彩、柳川右千夫、小幡邦彦、植野洋志、Margolskee RF、二ノ宮裕三
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] 味細胞における味覚の受容と情報伝達 (2009)
  • Author(s): 吉田竜介、村田芳博、安松啓子、重村憲徳、二ノ宮裕三
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 新潟
• [Presentation] 同一味質を呈す味物質に対する味細胞の応答性 (2009)
  • Author(s): 吉田竜介、宮内彩、安尾敏明、柳川右千夫、小幡邦彦、植野洋志 Margolskee RF、二ノ宮裕三
  • Organizer: 日本味と匂学会第43回大会
  • Place of Presentation: 旭川
• [Presentation] Taste responsiveness of Type II and III taste bud cellsin mouse fungiform papillae. (2009)
  • Author(s): Yoshida R, Yasuo T, Murata Y, Jyotaki M, Yanagawa Y, Obata K, Ueno H, Damak S, Margolskee RF, Ninomiya Y
  • Organizer: IUPS2009
  • Place of Presentation: Kyoto
• [Presentation] Different response properties between Type II and Type III taste bud cells in mouse fungiform papillae. (2009)
  • Author(s): Yoshida R, Yasuo T, Murata Y, Jyotaki M, Yanagawa Y, Obata K, Ueno H, Margolskee RF, Ninomiya Y
  • Organizer: AChemS
  • Place of Presentation: Sarasota, FL, USA
• [Remarks] 九州大学大学院歯学研究院口腔機能解析学分野HP
  • URL: http://www.dent.kyushu-u.ac.jp/sosiki/a06/index.html