Whole cell in vivo patch clamp analysis of Characteristic feature and network function of insular cortex.

• Project/Area Number: 21791811
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional basic dentistry
• Research Institution: Meikai University
• Principal Investigator: ADACHI Kazunori Meikai University, 歯学部, 講師 (20349963)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 神経科学 / in vivo / patch clamp / insular cortex / patch-clamP

Research Abstract

The spontaneous activity and stimulation induced membrane potential of rat's layer II/III pyramidal neurons in GI (granular) and DI (dysgranular) insular cortex were examined by in vivo patch clamp whole-cell system. The recorded cells were labled by biocytin to reconstruct the dendritic tree and axonal projections.

Research Products

• [Journal Article] Quantification of enhanced osteoblastic adhesion to ultraviolet-treated titanium plate. (2010)
  • Author(s): Contreras RG, Adachi K, Yokote Y, Sakagami H, Hibino Y, Nakajima H, Shimada J.
  • Journal Title: In Vivo Volume: 24 Pages: 519-523
  • NAID: 120005520736
  • Peer Reviewed
• [Journal Article] Purinergic receptors are involved in tooth-pulp evoked nocifensive behavior and brainstem neuronal activity. (2010)
  • Author(s): Adachi K, Shimizu K, Hu JW, Suzuki I, Sakagami H, Koshikawa N, Sessle BJ, Shinoda M, Miyamoto M, Honda K, Iwata K.
  • Journal Title: Molecular Pain Volume: 6
  • Peer Reviewed
• [Journal Article] Diurnal changes in salivary amino acid concentrations. (2010)
  • Author(s): Nakamura Y, Kodama H, Satoh T, Adachi K, Watanabe S, Yokote Y, Sakagami H.
  • Journal Title: In Vivo Volume: 24 Pages: 837-842
  • NAID: 120005520733
  • Peer Reviewed
• [Journal Article] Spatiotemporal dynamics of excitation in rat insular cortex : intrinsic corticocortical circuit regulates caudal-rostro excitatory propagation from the insular to frontal cortex. (2009)
  • Author(s): Fujita S, Adachi K, Koshikawa N, Kobayashi M.
  • Journal Title: Neuroscience Vol.167 Pages: 278-92
  • Peer Reviewed
• [Presentation] 島皮質錐体細胞の特性とネットワークの全動物パッチクランプ記録法による解析 (2010)
  • Author(s): 安達一典、小林真之、坂上宏、吉田篤、越川憲明
  • Organizer: 第4回三叉神経領域の感覚-運動統合機構研究会
  • Place of Presentation: 大阪府池田市伏尾町不死王閣
  • Year and Date: 2010-11-28
• [Presentation] 炎症性歯髄痛発症におけるP2X受容体サブタイプの関与 (2010)
  • Author(s): 安達一典、岩田幸一、越川憲明、James HU、 Barry Sessle、坂上宏
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 朱鷺メッセ(新潟)
  • Year and Date: 2010-03-16
• [Presentation] Analysis of masticatory movements in MPTP-induced hemi-parkinsonism monkeys. (2010)
  • Author(s): Adachi K, Kobayashi M, Koshikawa N.
  • Organizer: SYMPOSIUM 2010 : The Promotion of Multi-clisciplinary Research Projects "Translational Research Network on Orofacial Neurological Disorders (TRON projects)" & Japan-Canada Joint Health Research Program-U.Toronto/Nihon U.Grant"Multi-disciplinary approaches for conquest of oro-maxillofacial neurological disorders"
  • Place of Presentation: 日本大学歯学部(東京)
  • Year and Date: 2010-02-19
• [Presentation] Involvement of P2X receptors in tooth pulp pain. (2009)
  • Author(s): Adachi K, Iwata K, Koshikawa N, Hu JW, Sessle BJ, Sakagami H
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 朱鷺メッセ(新潟)
  • Year and Date: 2009-09-11
• [Presentation] Physiological features of pyramidal cells in rat insular cortex revealed by invivo whole-cell patch clamp recording. (2009)
  • Author(s): Adachi K, Fujita S, Sakagami H, Koshikawa N, Kobayashi M
  • Organizer: XXXVI International Congress of Physiological Sciences (IUPS 2009)
  • Place of Presentation: 京都国際会議場(京都)
  • Year and Date: 2009-07-31
• [Presentation] UNCTIONAL CONNECTIVITY IN RAT INSULAR, CORTEX REVEALEDBY IN VIVO OPTICAL IMAGING. (2009)
  • Author(s): Fujita S, Adachi K, Koshikawa N, Kobayashi M
  • Organizer: XXXVI International Congress of Physiological Sciences (IUPS 2009)
  • Place of Presentation: 京都国際会議場(京都)
  • Year and Date: 2009-07-31
• [Presentation] PHYSIOLOGICAL FEATURES OF PYRAMIDAL CELLS IN RAT INSULAR CORTEX REVEALED BY IN VIVO WHOLE-CELL PATCH CLAMP RECORDING. (2009)
  • Author(s): Kazunori Adachi, Satoshi Fujita, Hiroshi Sakagami, Noriaki Koshikawa, Masayuki Kobayashi
  • Organizer: The 36^<th> Congress of the International Union of Physiological Science (IUPS 2009).
• [Presentation] 島皮質錐体細胞の特性とネットワークの全動物パッチクランプ記録法による解析
  • Author(s): 安達一典、小林真之、坂上宏、吉田篤、越川憲明
  • Organizer: 第4回三叉神経領域の感覚-運動統合機構研究会
• [Book] 解かる!歯科薬理学 (2011)
  • Author(s): 坂上宏, 他
  • Total Pages: 353
  • Publisher: 学建書院
• [Remarks] ホームページ等