Molecular mechanisms of resistance to interferon-gamma in human oral squamous carcinoma.

• Project/Area Number: 21791812
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional basic dentistry
• Research Institution: Yokohama City University
• Principal Investigator: SEKINE Keisuke Yokohama City University, 医学部, 助教 (00323569)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 口腔生化学 / 口腔扁平上皮癌 / インターフェロン / 細胞周期 / Rb / サイクリン

Research Abstract

In this study, I examined the molecular mechanisms underlying the IFN-gamma dependent cell cycle arrest observed in human oral squamous cell carcinoma. I have previously revealed that IFN-gamma induced Rb activation results in cell cycle arrest. First, it is revealed that STAT1, a signal transducer of IFN-gamma mediate IFN-gamma dependent Rb activation. Second, it is revealed that there are several molecules that is up-regulated through IFN-gamma stimuli and which is observed up-stream of Rb activation.

Research Products

• [Journal Article] Reconstitution of hepatic tissue architectures from fetal liver cells obtained from a three-dimensional culture with a rotating wall vessel bioreactor. (2011)
  • Author(s): Ishikawa M, Sekine K, Okamura A, Zheng YW, Ueno Y, Koike N, Tanaka J, Taniguchi H.
  • Journal Title: J Biosci Bioeng. 111 Pages: 711-718
  • NAID: 110008672821
• [Journal Article] Reconstitution of hepatic tissue architectures from fetal liver cells obtained from a three-dimensional culture with a rotating wall vessel bioreactor. (2011)
  • Author(s): Ishikawa M, Sekine K, Okamura A, Zheng YW, Ueno Y, Koike N, Tanaka J, Taniguchi H., equal contribution.
  • Journal Title: J Biosci Bioeng. Volume: (Article in Press)
  • NAID: 110008672821
  • Peer Reviewed
• [Journal Article] Multiple Biological Complex of Alkaline Extract of the Leaves of Sasa senanensis Rehder. (2010)
  • Author(s): Sakagami H, Zhou L, Kawano M, Thet MM, Tanaka S, Machino M, Amano S, Kuroshita R, Watanabe S, Chu Q, Wang QT, Kanamoto T, Terakubo S, Nakashima H, Sekine K, Shirataki Y, Zhang CH, Uesawa Y, Mohri K, Kitajima M, Oizumi H, Oizumi T.
  • Journal Title: In Vivo 24 Pages: 735-743
  • NAID: 120005518460
• [Journal Article] Multiple Biological Complex of Alkaline Extract of the Leaves of Sasa senanensis Rehder. (2010)
  • Author(s): Sakagami H, Zhou L, Kawano M, Thet MM, Tanaka S, Machino M, Amano S, Kuroshita R, Watanabe S, Chu Q, Wang QT, Kanamoto T, Terakubo S, Nakashima H, Sekine K, Shirataki Y, Zhang CH, Uesawa Y, Mohri K, Kitajima M, Oizumi H, Oizumi T.
  • Journal Title: In Vivo Volume: 240 Pages: 735-743
  • NAID: 120005518460
  • Peer Reviewed
• [Journal Article] YB-1 downregulates expression of carbamoyl phosphate synthetase-I by suppressing C/EBPα function in mice (2009)
  • Author(s): Chen YR, Sekine K, Nakamura K, Yanai Y, Tanaka M, Miyajima A.
  • Journal Title: Gastroenterology 137 Pages: 330-340
• [Journal Article] A novel osteoclast precursor cell line, 4B12, recapitulates the features of primary osteoclast differentiation and function : enhanced transfection efficiency before and after differentiation. (2009)
  • Author(s): Amano S, Sekine K, Bonewald LF, Ohmori Y.
  • Journal Title: J Cell Physiol. 221 Pages: 40-53
• [Journal Article] Mechanisms of resistance to interferon-gamma-mediated cell growth arrest in human oral squamous carcinoma cells. (2009)
  • Author(s): Hiroi M, Mori K, Sekine K, Sakaeda Y, Shimada J, Ohmori Y.
  • Journal Title: J Biol Chem. 284 Pages: 24869-24880
• [Journal Article] YB-1 downregulates expression of carbamoyl phosphate synthetase-I by suppressing C/EBP α function in mice. (2009)
  • Author(s): Chen YR, Sekine K, Nakamura K, Yanai Y, Tanaka M, Miyajima A
  • Journal Title: Gastroenterology 137 Pages: 330-340
  • Peer Reviewed
• [Journal Article] A novel osteoclast precursor cell line, 4B12, recapitulates the features of primary osteoclast differentiation and function : enhanced transfection efficiency before and after differentiation. (2009)
  • Author(s): Amano S, Sekine K, Bonew ald LF, Ohmori Y
  • Journal Title: J Cell Physiol. 221 Pages: 40-53
  • Peer Reviewed
• [Presentation] 血管ネットワークを有したヒト肝組織の生体内再構築系の確立 (2010)
  • Author(s): 関根圭輔, 藤原綾二, 小池直人, 千葉豊生, Dai Fukumura, Rakesh K.Jain, 上野康晴, 鄭允文, 谷口英樹
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド(神戸)
  • Year and Date: 2010-12-10
• [Presentation] 転写因子Pdx1の可視化による膵幹/前駆細胞の選択的分離と特性解析 (2010)
  • Author(s): 関根圭輔, 石川桃太郎, 松井智栄美, 須崎敦大, 川下金明, 大島祐二, 鄭允文, 谷口英樹
  • Organizer: 日本バイオイメージング学会
  • Place of Presentation: 慶應義塾大学日吉キャンパス(横浜)
  • Year and Date: 2010-09-10
• [Presentation] Regulation of pRb but not p53 by IFNgamma in Human Oral Squamous Cell Carcinoma (2009)
  • Author(s): Sekine K, Ohmori Y.
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2009-12-12
• [Presentation] Regulation of pRb but not p53 by IFNgamma in Human Oral Spuamous Cell Carcinoma (2009)
  • Author(s): Sekine K, Ohmori Y.
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-12
• [Book] 現代栄養学を理解するための分子生物学入門(加藤茂明/編著) (2010)
  • Author(s): 関根圭輔
  • Publisher: 光生館
• [Book] 現代栄養学を理解するための 分子生物学入門(加藤茂明/編著) (2010)
  • Author(s): 関根圭輔
  • Publisher: 光生館