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Development of CXCL14/BRAK-derived anti-tumour peptide in head and neck squamous cell carcinoma.

Research Project

Project/Area Number 21791816
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionKanagawa Dental College

Principal Investigator

MAEHATA Yojiro  Kanagawa Dental College, 歯学部, 講師 (80410009)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords歯科薬理学 / 口腔癌 / CXCL14/BRAK / CXCL8/IL-8 / 血管新生抑制 / Drug Delivery System / 癌 / CXCL14 / BRAK / CXCL8 / IL-8
Research Abstract

BRAK, one of a non-ELR motif CXC chemokine, is also known as CXCL14. We previously reported that BRAK has a potent anti-tumor activity in head and neck squamous cell carcinoma cells (HNSCC), but the mechanism of action nor receptor for it is not known. In order to clarify the mechanism of tumor suppression by the BRAK we hypothesized that BRAK inhibits action of angiogenic cytokines such as IL-8 by directly binding to these cytokines. In the first year, we constructed FLAG-BRAK and FLAG-MOCK vectors and transfected these vectors into HSC-2 cells and obtained stably expressing these constructs, and named HSC-2/FRAG-BRAK, and HSC-2/FRAG-MOCK cells. Next year, in order to investigate binding capability of BRAK with these cytokines, we added recombinant proteins IL-8 or VEGF to the culture medium of HSC-2/FRAG-BRAK cells or HSC-2/FRAG-MOCK cells, and collected the culture medium. Following immunopurification of the culture medium, we investigated binding of BRAK with these cytokines and found that BRAK secreted into the culture medium bound with IL-8. Our results raise the possibility to develop angiogenesis inhibitory peptides by use of IL-8-binding peptides derived BRAK.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (23 results)

All 2011 2010 2009

All Journal Article (5 results) (of which Peer Reviewed: 3 results) Presentation (18 results)

  • [Journal Article] Reactive Oxygen Species (ROS) Reduce the Expression of BRAK/CXCL14in Human Head and Neck Squamous Cell Carcinoma Cells.2010

    • Author(s)
      Maehata Y, Ozawa S, Kobayashi K, Kato Y, Yoshino F, Miyamoto C, Izukuri K, Kubota E, Hata R-I, Lee M-C.
    • Journal Title

      Free Radical Res. 44(8)

      Pages: 913-924

    • Related Report
      2010 Final Research Report
  • [Journal Article] Reactive Oxygen Species (ROS) Reduce the Expression of BRAK/CXCL14in Human Head and Neck Squamous Cell Carcinoma Cells.2010

    • Author(s)
      Y MAEHATA, et al.
    • Journal Title

      Free Radical Research

      Volume: 44 Pages: 913-924

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Restoration of BRAK / CXCL14 gene expression by gefitinib is associated with antitumor efficacy of the drug in head and neck squamous cell carcinoma.2009

    • Author(s)
      Ozawa S, Kato Y, Ito S, Komori R, Shiiki N, Tsukinoki K, Ozono S, Maehata Y, Taguchi T, Imagawa-Ishiguro Y, Tsukuda M, Kubota E, Hata R.
    • Journal Title

      Cancer Sci. Nov.100(11)

      Pages: 2002-2009

    • NAID

      10027197383

    • Related Report
      2010 Final Research Report
  • [Journal Article] Restoration of BRAK/CXCL14 gene expression by gefitinib is associated with antitumor efficacy of the drug in head and neck sauamous cell carcinoma.2009

    • Author(s)
      Ozawa S, Maehata Y, et al.
    • Journal Title

      Cancer Science 100

      Pages: 2202-2209

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Roles of Collagen Molecules in Growth and Differentiation of Human Osteoblasts.2009

    • Author(s)
      Maehata Y, et al.
    • Journal Title

      Journal of Oral Bioscience 51

      Pages: 72-80

    • NAID

      10027104531

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] OXIDATIVE STRESS REDUCE EXPRESSION OF CXCL14/BRAK IN HUMAN SCC CELLS2011

    • Author(s)
      Y MAEHATA, et al.
    • Organizer
      89^<th> IADR/AADR General Session & Exhibition
    • Place of Presentation
      San Diego Convention Center
    • Year and Date
      2011-03-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] Oxidative stress reduce expression of CXCL14/BRAK in human cells.2011

    • Author(s)
      MAEHATA Y.,OZAWA S., MIYAMOTO C.,KOBAYASHI K.,KOMORI R.,HATA R.-I.,LEE M.C.-I.
    • Organizer
      IADR Gereral Session
    • Place of Presentation
      San Diego, Calif., USA.
    • Related Report
      2010 Final Research Report
  • [Presentation] Fasdil, an inhibitor of ROCK, suppresses tumor grouwth in vivo.2011

    • Author(s)
      MIYAMOTO C., MAEHATA Y., KOMORI R., KOBAYASHI K., HATA R.-I., LEE M.C.-I.
    • Organizer
      IADR Gereral Session
    • Place of Presentation
      San Diego, Calif., USA.
    • Related Report
      2010 Final Research Report
  • [Presentation] 新規抗腫〓性ケモカインBRAK/CXCL14の発現および分泌制御機構の解明~酸化ストレスはBRAKの発現と細胞外分泌を抑制する~2010

    • Author(s)
      前畑洋次郎, 宮本千央, 小澤重幸, 吉野文彦, 小林杏, 高橋聡子, 高橋俊介, 畑隆一郎, 李昌一
    • Organizer
      第25回日本酸化ストレス学会関東支部会
    • Place of Presentation
      江戸川, 東京
    • Year and Date
      2010-12-11
    • Related Report
      2010 Final Research Report
  • [Presentation] 新規抗腫〓性ケモカインBRAK/CXCL14の発現および分泌制御機構の解明~酸化ストレスはBRAKの発現と細胞外分泌を抑制する~.2010

    • Author(s)
      前畑洋次郎
    • Organizer
      第25回日本酸化ストレス学会関東支部会
    • Place of Presentation
      タワーホール船堀(シンポジウム)
    • Year and Date
      2010-12-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] 選択的ROCK阻害剤による抗腫〓性ケモカインBRAK/CXCL14を介した腫〓進展抑制の検討2010

    • Author(s)
      宮本千央, 前畑洋次郎, 小澤重幸, 生駒丈晴, 居作和人, 小林杏, 高橋聡子, 高橋俊介, 吉野文彦, 杉山秀太, 吉田彩佳, 畑隆一郎, 李昌一
    • Organizer
      神奈川歯科大学学会第45回総会
    • Place of Presentation
      横須賀, 神奈川
    • Year and Date
      2010-12-04
    • Related Report
      2010 Final Research Report
  • [Presentation] ROCK阻害剤Fasudil による抗腫〓性ケモカインBRAK/CXCL14の細胞外分泌促進と腫〓進展抑制の検討2010

    • Author(s)
      宮本千央, 前畑洋次郎, 小澤重幸, 生駒丈晴, 小森令賀, 居作和人, 加藤靖正, 畑隆一郎, 李昌一
    • Organizer
      第123回日本薬理学会関東部会
    • Place of Presentation
      自治医大、栃木
    • Year and Date
      2010-10-23
    • Related Report
      2010 Final Research Report
  • [Presentation] 選択的ROCK阻害剤による抗腫〓性ケモカインBRAK/CXCL14の腫〓進展抑制の検討2010

    • Author(s)
      宮本千央、前畑洋次郎、小澤重幸, 生駒丈晴, 居作和人, 畑隆一郎, 李昌一
    • Organizer
      神奈川歯科大学学会第133回例会
    • Place of Presentation
      横須賀、神奈川
    • Year and Date
      2010-10-14
    • Related Report
      2010 Final Research Report
  • [Presentation] 骨芽細胞の増殖と分化におけるコラーゲン分子の機能;骨コラーゲン代謝を標的とした骨粗鬆症治療の基礎的検討2010

    • Author(s)
      前畑洋次郎
    • Organizer
      第42回日本結合組織学会・第57回マトリックス研究会合同学術集会
    • Place of Presentation
      秋田市民交流プラザ(特別講演)
    • Year and Date
      2010-08-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] ROCK阻害剤はケモカインBRAK/CXCL14の細胞外分泌を促進し抗腫〓効果を発揮する2010

    • Author(s)
      宮本千央, 前畑洋次郎, 高橋聡子, 高橋俊介, 杉山秀太, 徳富文彬, 加藤靖正, 畑隆一郎, 李昌一
    • Organizer
      第52回歯科基礎医学会学術大会・総会
    • Place of Presentation
      江戸川,東京
    • Related Report
      2010 Final Research Report
  • [Presentation] ROCK阻害剤FasudilはケモカインBRAK/CXCL14の細胞外分泌を促進し抗腫〓効果を発揮する2010

    • Author(s)
      宮本千央, 前畑洋次郎, 小澤重幸, 加藤靖正, 畑隆一郎, 李昌一
    • Organizer
      第42回日本結合組織学会・第57回マトリックス研究会合同学術集会
    • Place of Presentation
      秋田, 秋田
    • Related Report
      2010 Final Research Report
  • [Presentation] 口腔扁平上皮癌細胞においてGefitinib (Iressa)は酸化ストレスに誘導される抗腫瘍性ケモカインBRAK/CXCL14の発現低下を抑制する2009

    • Author(s)
      前畑洋次郎, 小澤重幸, 小林杏, 吉野文彦, 宮本千央, 加藤靖正, 畑隆一郎, 李昌一
    • Organizer
      第121回日本薬理学会関東部会
    • Place of Presentation
      新宿区,東京
    • Year and Date
      2009-10-10
    • Related Report
      2010 Final Research Report
  • [Presentation] 口腔扁平上皮癌細胞においてGefitinib(Iressa)は酸化ストレスに誘導される抗腫瘍性ケモカインBRAK/CXCL 14の発現低下を抑制する2009

    • Author(s)
      前畑洋次郎
    • Organizer
      第121回日本薬理学会関東部会
    • Place of Presentation
      新宿区,東京
    • Year and Date
      2009-10-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] Reactive oxygen species reduce the expression of BRAK/CXCL 14 in human head and neck sauamous cell carcinoma cells.2009

    • Author(s)
      Maehata Y., et al.
    • Organizer
      41st Annual Meeting of the Japanese Society of Connective Tissue Research (JSCTR) 56th Annual Meeting of the Japan Matricx Club (JMC)
    • Place of Presentation
      Yokosuka, Kanagawa, Japan.
    • Year and Date
      2009-06-06
    • Related Report
      2009 Annual Research Report
  • [Presentation] 頭頚部扁平上皮癌細胞種における酸化ストレスによる腫瘍抑制ケモカインBRAKICXCL14の発現抑制制御機構の検討2009

    • Author(s)
      宮本千央, 前畑洋次郎
    • Organizer
      第9回日本抗加齢医学会総会
    • Place of Presentation
      港区,東京
    • Year and Date
      2009-05-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] Reactive oxygen species reduce the production of BRAK/CXCL14 in human head and neck squamous cell carcinoma cells.2009

    • Author(s)
      MIYAMOTO C., MAEHATA Y., YOSHINO F., KOBAYASHI K., LEE M.C.-I.
    • Organizer
      SFRBM's 16th Annual Meeting
    • Place of Presentation
      San Francisco,California USA.
    • Related Report
      2010 Final Research Report
  • [Presentation] Reactive oxygen species reduce the expression of BRAK/CXCL14 in human head and neck squamous cell carcinoma cells.2009

    • Author(s)
      MAEHATA Y., OZAWA S., MIYAMOTO C., KOBAYASHI K., KATO Y., YOSHINO F., LEE M.C.-I., HATA R.
    • Organizer
      41st Annual Meeting of the Japanese Society of Connective Tissue Research (JSCTR) 56th Annual Meeting of the Japan Matricx Club (JMC)
    • Place of Presentation
      Yokosuka, Kanagawa, Japan.
    • Related Report
      2010 Final Research Report
  • [Presentation] 頭頚部扁平上皮癌細胞種における酸化ストレスによる腫瘍抑制ケモカインBRAK/CXCL14の発現抑制制御機構の検討2009

    • Author(s)
      宮本千央, 前畑洋次郎, 吉野文彦, 小林杏, 吉田彩佳, 杉山秀太, 高橋聡子, 高橋俊介, 前谷崇志, 川村陽介, 小松知子, 岡田永三, 岡田康江, 辻村傑, 大野晃教, 李昌一
    • Organizer
      第9回日本抗加齢医学会総会
    • Place of Presentation
      港区,東京
    • Related Report
      2010 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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