Next-generation method with electrophoresis in microRNA expression profiling

• Project/Area Number: 21791819
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional basic dentistry
• Research Institution: Asahi University
• Principal Investigator: HAYASHI Toru Asahi University, 歯学部, 助教 (10454266)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: マイクロRNA / 変性剤濃度勾配ゲル電気泳動法

Research Abstract

The purpose of this study was to establish a new method to analyze microRNAs (miRNAs) expression profile. In denaturing gradient gel electrophoresis DNA fragments of the same length but with different base-pair sequences can be separated. This method enables us to detect miRNAs including novel sequences and to distinguish single nucleotide difference among miRNAs. Then, I attempted to detect miRNAs showing specific expression pattern among multiple samples by this electrophoresis. This method distinguished tiny sequences variation successfully, suggesting that it is probable to detect miRNA variants.

Research Products

• [Journal Article] Mesenchymal miR-21 regulates branching morphogenesis in murine submandibular gland in vitro. (2011)
  • Author(s): Toru Hayashi, Noriko Koyama, Yukio Azuma, Masanori Kashimata.
  • Journal Title: Developmental Biology 352 Pages: 299-307
• [Journal Article] Mesenchymal miR-21 regulates branching morphogenesis in murine submandibular gland in vitro (2011)
  • Author(s): Toru Hayashi
  • Journal Title: Developmental Biology Volume: 352 Pages: 299-307
  • Peer Reviewed
• [Journal Article] Detection of EGF-dependent microRNAs of the fetal mouse submandibular gland at embryonic day 13 (2009)
  • Author(s): Toru Hayashi
  • Journal Title: The Journal of Medical Investigation 56, Supplement Pages: 250-252
  • NAID: 120006472136
• [Presentation] Mesenchymal miR-21 regulates branching morphogenesis in murine submandibular gland in vitro (2011)
  • Author(s): Toru Hayashi
  • Organizer: Salivary Gland and Exocrine Biology Gordon Research Conference
  • Place of Presentation: Galveston, Texas
  • Year and Date: 2011-02-06 – 2011-02-11
• [Presentation] Mesenchymal miR-21 regulates branching morphogenesis in murine submandibular gland in vitro. (2011)
  • Author(s): Toru Hayashi, Noriko Koyama, Yukio Azuma, Masanori Kashimata.
  • Organizer: Salivary Gland and Exocrine Biology Gordon Research Conference 2011.
  • Place of Presentation: Galveston, Texas
• [Presentation] Mesenchymal miR-21 regulates branching morphogenesis in murine submandibular gland in vitro (2011)
  • Author(s): Toru Hayashi
  • Organizer: Salivary Gland and Exocrine Biology Cordon Research Conference
  • Place of Presentation: Galveston, Texas
• [Presentation] 胎仔マウス顎下腺マイクロRNAのEGF処理による発現上昇 (2010)
  • Author(s): 林徹
  • Organizer: 第52回歯科基礎医学会学術大会
  • Place of Presentation: タワーホール船堀、東京
  • Year and Date: 2010-09-21
• [Presentation] 胎仔マウス顎下腺マイクロRNAのEGF処理による発現上昇 (2010)
  • Author(s): 林 徹
  • Organizer: 第52回 歯科基礎医学会学術大会
  • Place of Presentation: タワーホール船堀、東京
  • Year and Date: 2010-09-21
• [Presentation] 胎仔マウス顎下腺のマイクロRNAプロファイリング (2009)
  • Author(s): 林徹
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 朱鷺メッセ 新潟コンベンションセンター(新潟県)
  • Year and Date: 2009-09-11