Shh signaling is related to Bmp signaling in ameloblast differentiation and tooth reconstruction

• Project/Area Number: 21791838
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Conservative dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: TAKAHASHI Satomi 東京医科歯科大学, 大学院・医歯学総合研究科, 非常勤講師 (40451918)
• Co-Investigator(Renkei-kenkyūsha): KAWASHIMA Nobuyuki 東京医科歯科大学, 大学院・医歯学総合研究科, 助教 (60272605)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 歯の発生 / エナメル芽細胞 / Sonic hedgehog / Gli / Bone morphogenetic protein / Smad / Wnt / 歯の再生 / BMP / Bmp / クロストーク

Research Abstract

Tooth development is regulated by various intercellular signaling molecules, such as sonic hedgehog (Shh), bone morphogenetic proteins (BMPs), Wnts and fibloblasts growth factors. These molecules are also essential for ameloblast differentiation. We reported that Shh signaling was indispensable for ameloblast development. In ameloblast, Shh, BMPs and Wnts expressions are observed in their early stage of development, along with their receptor molecules. These observations suggest the involvement of Shh, BMPs and Wnts signaling in the differentiation of ameloblasts. We revealed that Shh induced the expression of BMPs and Wnts, suggesting that Shh may play a central role to activate the signaling network in ameloblasts.

Research Products

• [Journal Article] Expression of Notch-Signalling-related Genes in Normal and Differentiating Rat Dental Pulp Cells (2010)
  • Author(s): Sun H, Kawashima N, Xu J, Takahashi S, Suda H
  • Journal Title: Australian Endodontic Journal (In press)
  • Peer Reviewed
• [Presentation] In Vitro Ameloblast Differentiation Affected by Wnt Signaling (2011)
  • Author(s): Satomi Takahashi
  • Organizer: European Society of Endodontrogy
  • Place of Presentation: イタリアローマ
  • Year and Date: 2011-09-15
• [Presentation] In Vitro Ameloblast Differentiation Affected by Wnt Signaling (2011)
  • Author(s): Satomi Takahashi
  • Organizer: 15^<TH> BIENNIAL CONGRESS OF THE EUROPEAN SOCIETY OF ENDODONTROGY
  • Place of Presentation: ROME
• [Presentation] Shh Signaling is Related to BMP and Wnt Signaling in Ameloblast Differentiation (2010)
  • Author(s): Satomi Takahashi
  • Organizer: International Federation of Endodontic Associations
  • Place of Presentation: ギリシャアテネ
  • Year and Date: 2010-10-07
• [Presentation] SHH SIGNALING IS RELETED TO BMP AND WNT SIGNALING IN AMELPBLAST DIFFERENTIATION (2010)
  • Author(s): 高橋里美
  • Organizer: IFEA 8^<th> world congress
  • Place of Presentation: ギリシャ(アテネ)
  • Year and Date: 2010-10-07
• [Presentation] nduction of IL17 synthesis in the experimentally-induced murine periapical lesions (2010)
  • Author(s): Kawashima N., Xu J., Suzuki N., Zhou M., Wei S., Takimoto K., Koizumi Y., Takahashi S., Suda H.
  • Organizer: Spring Scientific Meeting of Korean Academy of Endodontics
  • Place of Presentation: BEXCO, Busan, Korea
  • Year and Date: 2010-03-27
• [Presentation] 象牙芽細胞分化におけるSp7による dentin sialophosphoprotein 発現誘導 (2010)
  • Author(s): 川島伸之、許〓、岩田隆紀、周夢宇、瀧本晃陽、小泉悠、大井智恵、高橋里美、鈴木規元、須田英明
  • Organizer: 日本再生医療学会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2010-03-18
• [Presentation] Expression of Sp7 in the rat dental pulp (2009)
  • Author(s): 川島伸之, 周夢宇, 瀧本晃陽, 小泉悠, 大井智恵, 高橋里美, 鈴木規元, 須田英明
  • Organizer: Autumn Scientific Meeting of Korean Academy of Conservative Dentistry
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2009-11-13
• [Presentation] Expression of Th17 related cytokines in experimentally-induced rat periapical lesions (2009)
  • Author(s): Wei S Kawashima N, Suzuki N, Xu J, Takahashi S, Zhou M, Koizumi Y, Suda H
  • Organizer: Asian Pacific Endodontic Confederation
  • Place of Presentation: Toshi center Hotel Tokyo
  • Year and Date: 2009-04-25
• [Presentation] VIP receptor expression in tooth development (2009)
  • Author(s): Xu J, Kawashima N, Suzuki N, Okuhara S, Wei S, Takahashi S, Zhou M, Koizumi Y, Suda H
  • Organizer: Asian Pacific Endodontic Confederation
  • Place of Presentation: Toshi center Hotel Tokyo
  • Year and Date: 2009-04-25