Characterization of stem cells from human exfoliated deciduous teeth (SHED) for mineralized tissue regeneration

• Project/Area Number: 21791848
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Conservative dentistry
• Research Institution: Nagoya University
• Principal Investigator: NAKAMURA Sayaka Nagoya University, 医学部附属病院, 医員 (40437033)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 再生医療 / 歯髄 / 乳歯 / 幹細胞 / 組織工学

Research Abstract

Stem cells from human exfoliated deciduous teeth (SHED) have been identified as a novel population of stem cells. In this study, we focused on the characterization and possibility of mineralized tissue regeneration using SHED. These results suggested that SHED possesses adequate osteogenic/odontogenic capacity and is able to provide enough number of cells for clinical application easily because of high proliferation capacity. SHED that is generally discarded as medical waste and can be obtained without any invasion could be useful cell source for mineralized tissue formation.

Research Products

• [Journal Article] Regulation of the Wnt signaling pathways during cell culture of human mesenchymal stem cells for efficient bone regeneration. (2011)
  • Author(s): Katagiri W., Yamada Y., Nakamura S., Ito K., Hara K., Hibi H., Ueda M.
  • Journal Title: Oral science Int. in press.
  • NAID: 10027680495
  • Peer Reviewed
• [Journal Article] Stem cells from human exfoliated deciduous teeth (SHED) enhance wound healing and possibility of novel cell therapy. (2011)
  • Author(s): Nishino, Y
  • Journal Title: Cytotherapy Volume: (In press)
  • Peer Reviewed
• [Journal Article] Regulation of the Wnt signaling pathways during cell culture of human mesenchymal stem cells for efficient bone regeneration. (2011)
  • Author(s): Katagiri, W
  • Journal Title: Oral science Int Volume: (In press)
  • Peer Reviewed
• [Journal Article] Periodontal perspectives from Asia : Current and future periodontal tissue engineering. (2011)
  • Author(s): Izumi, Y
  • Journal Title: Periodontol 2000 Volume: (In press)
  • Peer Reviewed
• [Journal Article] Promising cell-based therapy for bone regeneration using stem cells from deciduous teeth, dental pulp, and bone marrow. (2010)
  • Author(s): Yamada Y., Ito K., Nakamura S., Ueda M., Nagasaka T.
  • Journal Title: Cell Transplant. Nov 5(Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Stem cells from human exfoliated deciduous teeth (SHED) enhance wound healing and possibility of novel cell therapy. (2010)
  • Author(s): Nishino Y., Yamada Y., Ebisawa K., Nakamura S., Okabe K., Umemura E., Hara K., Ueda M.
  • Journal Title: Cytotherapy in press.
  • Peer Reviewed
• [Journal Article] A feasibility of useful cell-based therapy by bone regeneration with deciduous tooth stem cells, dental pulp stem cells, or bone marrow-derived mesenchymal stem cells for clinical study using tissue engineering technology. (2010)
  • Author(s): Yamada Y, Nakamura S, Ito K, Sugito T, Yoshimi R, Nagasaka T, Ueda M.
  • Journal Title: Tissue Eng Part A. (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Promising cell-based therapy for bone regeneration using stem cells from deciduous teeth, dental pulp, and bone marrow. (2010)
  • Author(s): Yamada, Y
  • Journal Title: Cell Transplant Volume: (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] A feasibility of useful cell-based therapy by bone regeneration with deciduous tooth stem cells, dental pulp stem cells, or bone marrow-derived mesenchymal stem cells for clinical study using tissue engineering technology. (2010)
  • Author(s): Yamada, Y
  • Journal Title: Tissue Eng Part A. (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Stem Cell Proliferation Pathways Comparison Between Human Exfoliated Deciduous Teeth and Dental Pulp Stem Cells by Gene Expression Profile From Promising Dental Pulp. (2009)
  • Author(s): Nakamura S., Yamada Y., Katagiri W., Sugito T., Ito K., Ueda M.
  • Journal Title: J Endod. Nov;35(11) Pages: 1536-1542
  • Peer Reviewed
• [Journal Article] Stem Cell Proliferation Pathways Comparison Between Human Exfoliated Deciduous Teeth and Dental Pulp Stem Cells by Gene Expression Profile From Promising Dental Pulp (2009)
  • Author(s): Nakamura, S
  • Journal Title: J Endod 35(11) Pages: 1536-1542
  • Peer Reviewed
• [Journal Article] Self-assembling peptide nanofiber scaffolds, platelet rich plasma, and mesenchymal stem cells for injectable bone regeneration with tissue engineering. (2009)
  • Author(s): Yoshimi, R
  • Journal Title: J Craniofac Surg 20(5) Pages: 1523-1530
  • Peer Reviewed
• [Journal Article] 乳歯幹細胞研究バンク (2009)
  • Author(s): 中村さやか
  • Journal Title: 総合臨床 58 Pages: 33-35
• [Presentation] 遺伝子プロファイリングによる骨髄幹細胞と歯髄幹細胞のキャラクタライズスタディー (2010)
  • Author(s): 原憲史
  • Organizer: 第55回日本口腔外科学会総会
  • Place of Presentation: 千葉
  • Year and Date: 2010-10-17
• [Presentation] Cell-based therapy with dental pulp stem cells for bone regeneration. (2010)
  • Author(s): Yamada, Y., Nakamura, S., Ito, K., Katagiri, W., Umemura, E., Hara, K., Hibi, H., Ueda, M.
  • Organizer: International Association for Dental Research (88nd General session of the pan European region of IADR), Barcelona
  • Place of Presentation: Spain
  • Year and Date: 2010-07-17
• [Presentation] Proliferation of SHED and relation to Wnt signaling pathway. (2010)
  • Author(s): Katagiri, W., Yamada, Y., Nakamura, S., Ito, K., Katagiri, W., Umemura, E., Hara, K., Hibi, H., Ueda, M.
  • Organizer: International Association for Dental Research (88nd General session of the pan European region of IADR)
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-17
• [Presentation] Cell-based therapy with dental pulp stem cells for bone regeneration. (2010)
  • Author(s): Yamada, Y
  • Organizer: International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-17
• [Presentation] Proliferation of SHED and relation to Wnt signaling pathway. (2010)
  • Author(s): Katagiri, W
  • Organizer: International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-17
• [Presentation] Optimized study for dental pulp stem cell-based therapy by ready-to-use. (2010)
  • Author(s): Umemura, E., Yamada, Y., Nakamura, S., Ito, K., Katagiri, W., Hara, K., Ueda, M.
  • Organizer: International Association for Dental Research (88nd General session of the pan European region of IADR)
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-16
• [Presentation] Optimized study for dental pulp stem cell-based therapy by ready-to-use. (2010)
  • Author(s): Umemura, E
  • Organizer: International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-16
• [Presentation] Characterization of dental pulp stem cells for mineralized tissue regeneration. (2010)
  • Author(s): Nakamura, S., Yamada, Y., Katagiri, W., Ito, K., Umemura, E., Ueda, M.
  • Organizer: International Association for Dental Research (88nd General session of the pan European region of IADR)
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-15
• [Presentation] Characterization of dental pulp stem cells for mineralized tissue regeneration. (2010)
  • Author(s): Nakamura, S
  • Organizer: International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-15
• [Presentation] 乳歯および永久歯歯髄細胞による骨再生・インプラント治療への応用 (2010)
  • Author(s): 中村さやか
  • Organizer: 第132回日本歯科保存学会春季学術大会
  • Place of Presentation: 熊本
  • Year and Date: 2010-06-04
• [Presentation] 乳歯および永久歯歯髄細胞による骨再生・インプラント治療への応用 (2010)
  • Author(s): 中村さやか, 山田陽一, 伊藤憲治, 上田実
  • Organizer: 第132回日本歯科保存学会学術大会
  • Place of Presentation: 熊本
• [Presentation] 乳歯・永久歯由来歯髄幹細胞による顎顔面領域再生への可能性 (2009)
  • Author(s): 山田陽一, 伊藤憲治, 中村さやか, 片桐渉, 杉戸孝行, 吉見涼子, 西野雄大, 日比英晴, 上田実
  • Organizer: 第54回日本口腔外科学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2009-10-10
• [Presentation] ヒト乳歯歯髄由来幹細胞の細胞特性の検討:増殖能とWnt/βカテニン経路の関連 (2009)
  • Author(s): 片桐渉
  • Organizer: 第54回日本口腔外科学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2009-10-10
• [Presentation] 乳歯歯髄幹細胞による皮膚創傷治癒効果の検討 (2009)
  • Author(s): 西野雄大
  • Organizer: 第54回日本口腔外科学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2009-10-10
• [Presentation] Characterization and possibility of bone regeneration for dental implant using dental pulp stem cells (DPSCs) and stem cells from human exfoliated deciduous teeth (SHED) as novel stem cell origin. (2009)
  • Author(s): Nakamura S., Yamada Y., Katagiri W., Sugito T., Ito K., Ueda M.
  • Organizer: 18th Annual Scientific Meeting of European Association for Osseointegration
  • Place of Presentation: Monaco
• [Presentation] Clinical application using injectable tissueengineered bone for minimally invasive and aesthetic dental implant treatment. (2009)
  • Author(s): Yamada Y., Ito K., Nakamura S., Yoshimi R., Hibi H., Baba S., Ueda M.
  • Organizer: 18th Annual Scientific Meeting of European Association for Osseointegration
  • Place of Presentation: Monaco
• [Presentation] 歯髄幹細胞による再生医療を用いたインプラント治療への応用-乳歯、永久歯歯髄幹細胞による骨再生パラダイムシフト- (2009)
  • Author(s): 山田陽一, 伊藤憲治, 中村さやか, 片桐渉, 馬場俊輔, 上田実
  • Organizer: 第39回日本口腔インプラント学会総会
  • Place of Presentation: 大阪
• [Presentation] 乳歯および永久歯歯髄細胞の遺伝子発現プロファイルの比較検討 (2009)
  • Author(s): 中村さやか, 山田陽一, 片桐渉, 杉戸孝行, 伊藤憲治, 上田実
  • Organizer: 第130回日本歯科保存学会学術大会
  • Place of Presentation: 札幌
• [Presentation] 歯髄幹細胞による創傷治癒、骨再生医療への展開-乳歯・永久歯歯髄幹細胞の比較- (2009)
  • Author(s): 山田陽一, 中村さやか, 片桐渉, 杉戸孝行, 西野雄大, 伊藤憲治, 上田実
  • Organizer: 第130回日本歯科保存学会学術大会
  • Place of Presentation: 札幌
• [Presentation] 幹細胞を用いた再生医療による新規歯周病治療法のパラダイムシフト-臨床経過から- (2009)
  • Author(s): 山田陽一、中村さやか、伊藤憲治、日比英晴、上田実
  • Organizer: 第52回日本歯周病学会学術大会
  • Place of Presentation: 岡山
• [Presentation] Characterization and possibility of bone regeneration for dental implant using dental pulp stem cells (DPSCs) and stem cells from human exfoliated deciduous teeth (SHED) as novel stem cell origin (2009)
  • Author(s): Nakamura, S
  • Organizer: 18th Annual Scientific Meeting of European Association for Osseointegration
  • Place of Presentation: Monaco
• [Presentation] Clinical application using injectable tissueengineered bone for minimally invasive and aesthetic dental implant treatment (2009)
  • Author(s): Yamada, Y
  • Organizer: 18th Annual Scientific Meeting of European Association for Osseointegration
  • Place of Presentation: Monaco
• [Presentation] 歯髄幹細胞による創傷治癒、骨再生医療への展開-乳歯・永久歯歯髄幹細胞の比較 (2009)
  • Author(s): 山田陽一
  • Organizer: 第130回日本歯科保存学会学術大会
  • Place of Presentation: 札幌
• [Presentation] 歯髄幹細胞による再生医療を用いたインプラント治療への応用 -乳歯、永久歯歯髄幹細胞による骨再生パラダイムシフト- (2009)
  • Author(s): 山田陽一
  • Organizer: 第39回日本口腔インプラント学会総会
  • Place of Presentation: 大阪
• [Book] 実験医学(再生医療の最前線2010幹細胞の分化誘導と臨床応用 歯髄幹細胞の再生医療における可能性)(第3章) (2010)
  • Author(s): 山田陽一、中村さやか、上田実
  • Publisher: 羊土社
• [Book] 実験医学 再生医療の最前線2010 第3章 幹細胞の分化誘導と臨床応用 歯髄幹細胞の再生医療における可能性 (2010)
  • Author(s): 山田陽一
  • Publisher: 羊土社
• [Book] 実験医学再生医療の最前線2010 第3章 幹細胞の分化誘導と臨床応用 歯髄幹細胞の再生医療における可能性 (2010)
  • Author(s): 山田陽一
  • Publisher: 羊土社
• [Patent(Industrial Property Rights)] 乳歯歯髄幹細胞に特徴的な遺伝子発現群の利用 (2009)
  • Inventor(s): 山田陽一、中村さやか、上田実
  • Industrial Property Rights Holder: 名古屋大学
  • Filing Date: 2009-05-20
• [Patent(Industrial Property Rights)] 乳歯歯髄幹細胞に特徴的な遺伝子発現群の利用 (2009)
  • Inventor(s): 山田陽一、中村さやか、上田実
  • Industrial Property Rights Holder: 名古屋大学
  • Industrial Property Number: 2009-122379
  • Filing Date: 2009-05-20