Nobel bone regenerative medicine promoted by regulation of human MSC by Wnt/sFRP family

• Project/Area Number: 21791985
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Nagoya University
• Principal Investigator: KATAGIRI Wataru Nagoya University, 医学系研究科, 助教 (10437030)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: Wntシグナル / 骨再生医療 / 幹細胞 / MSC / sFRP-3 / 未分化間葉系幹細胞 / 組織工学 / 細胞培養

Research Abstract

Recently it has been revealed that Wnt signaling pathway regulates the osteoblastic differentiation of human mesenchymal stem cells (hMSCs). We used sFRP-3, the antagonist of Wnt, to regulate this signaling and investigated the effect on osteoblastic differentiation of hMSCs in vitro and in vivo. It was revealed that the addition of sFRP-3 showed early differentiation and mineralization of hMSCs, and also showed early bone regeneration in animal model. These findings indicated the possibility of novel bone regenerative medicine using sFRP-3.

Research Products

• [Journal Article] Regulation of the canonical Wnt signaling pathways during cell culture of human mesenchymal stem cells for efficient bone regeneration (2010)
  • Author(s): Katagiri W, Yamada Y, Nakamura S, Ito K, Hara K, Hibi H, Ueda M.
  • Journal Title: Oral Science International 7 Pages: 37-46
  • Peer Reviewed
• [Journal Article] Involvement of the Wnt-β-catenin pathway in invasion and migration of oral squamous carcinoma cells (2010)
  • Author(s): Iwai S, Yonekawa A, Harada C, Hamada M, Katagiri W, Nakazawa M, Yura Y.
  • Journal Title: International Journal of Oncology 37 Pages: 1095-1103
  • Peer Reviewed
• [Journal Article] Regulation of the canonical Wnt signaling pathways during cell culture of human mesenchymal stem cells for efficient bone regeneration (2010)
  • Author(s): Katagiri W, Yamada Y, Nakamura S, Ito K, Hara K, Hibi H, Ueda M
  • Journal Title: Oral Science International Volume: 7 Pages: 37-46
  • Peer Reviewed
• [Journal Article] Involvement of the Wnt-B-catenin pathway in invasion and migration of oral squamous carcinoma cells (2010)
  • Author(s): Iwai S, Yonekawa A, Harada C, Hamada M, Katagiri W, Nakazawa M, Yura Y
  • Journal Title: International Journal of Oncology Volume: 37 Pages: 1095-1103
  • Peer Reviewed
• [Journal Article] Regulation of the Wnt signaling pathways during cell culture of human mesenchymal stem cells for efficient bone regeneration (2010)
  • Author(s): Katagiri W
  • Journal Title: Oral Science International (In press)
  • NAID: 10027680495
  • Peer Reviewed
• [Journal Article] Stem cell proliferation pathways comparison between human exfoliated deciduous teeth and dental pulp stem cells by gene expression profile from promising dental pulp (2009)
  • Author(s): Nakamura S
  • Journal Title: Journal of Endodontics 35 Pages: 1536-1542
  • Peer Reviewed
• [Presentation] 歯髄幹細胞による再生医療を用いたインプラント治療への応用 -乳歯、永久歯歯髄幹細胞による骨再生パラダイムシフト- (2009)
  • Author(s): 山田陽一
  • Organizer: 第39回日本口腔インプラント学会総会
  • Place of Presentation: 大阪市
  • Year and Date: 2009-11-27
• [Presentation] ヒト乳歯歯髄由来幹細胞の細胞特性の検討Wnt/β-カテニン経路の関連 (2009)
  • Author(s): 片桐渉
  • Organizer: 第54回日本口腔外科学会総会・学術大会
  • Place of Presentation: 札幌市
  • Year and Date: 2009-10-10
• [Presentation] 乳歯・永久歯歯髄幹細胞による顎顔面領域再生への可能性 (2009)
  • Author(s): 山田陽一
  • Organizer: 第54回日本口腔外科学会総会・学術大会
  • Place of Presentation: 札幌市
  • Year and Date: 2009-10-10
• [Presentation] Characterization and possibility of bone regeneration for dental implant using dental pulp stem cells (DPSCs) and stem cells from human exfoliated deciduous teeth (SHED) as novel stem cell orgin (2009)
  • Author(s): Nakamura S
  • Organizer: 18^<th> Annual Scientific Meeting European Association for Osseointegration
  • Place of Presentation: Monaco
  • Year and Date: 2009-10-01
• [Presentation] 乳歯および永久歯歯髄細胞の遺伝子発現プロファイルの比較検討 (2009)
  • Author(s): 中村さやか
  • Organizer: 第130回日本歯科保存学会学術大会
  • Place of Presentation: 札幌市
  • Year and Date: 2009-06-11