siRNA therapy targeting DIF・PDE1 signal transduction pathway on human malignant melanoma

Research Project

Project/Area Number	21791986
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Surgical dentistry
Research Institution	Mie University
Principal Investigator	SHIMIZU Kasumi Mie University, 医学部附属病院, 助教 (20378368)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	Phosphodiesterase / 悪性黒色腫 / phosphodiesterase 1 / 運動能
Research Abstract	We analyzed the effect of PDE1A siRNA and PDE1C siRNA for human malignant melanoma cell migration. Both of them increased cell migration slightly. And, PDE1 did not relate apoptosis. But, PDE2 related the DNA synthesis and it suggested that PDE2 may regulates the cell cycle. Furthermore, PDE1 specific inhibitor inhibits the expression of MMP2, but did not inhibit expression of MMP3 and MMP8.

Report

(3 results)