Elucidation of mechanism underlying 5-FU resistance in oral cancer using genomic analysis and application to multimodal personalized medicine

• Project/Area Number: 21792017
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Kumamoto University
• Principal Investigator: NAKAYAMA Hideki Kumamoto University, 医学部附属病院, 助教 (70381001)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 臨床腫瘍学 / 抗癌剤耐性 / 口腔扁平上皮がん / 5-フルオロウラシル / アポトーシス / cIAP2 / 口腔扁平上皮癌 / TS

Research Abstract

In the present study, to identify novel molecules associated with the 5-FU resistance of OSCC, we established a 5-FU-resistant OSCC cell line over a 2-year period, and found that an overexpression of cIAP2 confers 5-FU resistance in the cells. Furthermore, an immunohistochemical analysis using OSCC patient tissue samples demonstrated that increased expression of cIAP2 resulted in enhanced resistance to 5-FU-based chemoradiotherapy and a poor prognosis.

Research Products

• [Journal Article] Haemophilic pseudotumour of the mandible in a 5-year-old patient. (2011)
  • Author(s): Yoshitake Y, Nakayama H, Takamune Y, Yasunaga M, Hiraki A, Shinohara M.
  • Journal Title: Int J Oral Maxillofac Surg. Volume: 40 Pages: 120-3
  • Peer Reviewed
• [Journal Article] Phase II Study of Preoperative Concurrent Chemoradiation Therapy with S-1 in Patients with T4 Oral Squamous Cell Carcinoma. (2010)
  • Author(s): Nomura T, Murakami R, Toya R, Teshima K, Nakahara A, Hirai T, Hiraki A, Nakayama H, Yoshitake Y, Ota K, Obayashi T, Yamashita Y, Oya N, Shinohara M.
  • Journal Title: Int J Radiat Oncol Biol Phys. 76(5) Pages: 1347-1352
  • Peer Reviewed
• [Journal Article] Radiation-induced parotid gland changes in oral cancer patients : correlation between parotid volume and saliva production. (2010)
  • Author(s): Teshima K, Murakami R, Tomitaka E, Nomura T, Toya R, Hiraki A, Nakayama H, Hirai T, Shinohara M, Oya N, Yamashita Y.
  • Journal Title: Jpn J Clin Oncol. 40(1) Pages: 42-46
  • Peer Reviewed
• [Journal Article] 歯性感染症が原因で生じたと考えられた敗血症性肺塞栓症の1例 (2010)
  • Author(s): 吉田遼司、中山秀樹、永田将士、吉武義泰、手島慶子、篠原正徳
  • Journal Title: 日本口腔外科学会雑誌 56 Pages: 49-53
  • NAID: 10027842203
  • Peer Reviewed
• [Journal Article] PTCH1遺伝子のミスセンス変異が認められた母斑基底細胞癌症候群の1例 (2010)
  • Author(s): 吉武義泰、中山秀樹、福間大喜、尾木秀直、平木昭光、篠原正徳
  • Journal Title: 日本口腔外科学会雑誌 56 Pages: 40-44
  • NAID: 10027844265
  • Peer Reviewed
• [Journal Article] 8歳女児の下顎骨骨折に対して観血的整復固定術と保存的治療を併用した1例 (2010)
  • Author(s): 高宗康隆、中山秀樹、難波亜弥、福間大喜、田中拓也、永田将士、吉武義泰、尾木秀直、篠原正徳
  • Journal Title: 日本口腔顎顔面外傷学会誌 9(2) Pages: 109-114
  • Peer Reviewed
• [Journal Article] Phase II Study of Preoperative Concurrent Chemoradiation Therapy with S-1 in Patients with T4 Oral Squamous Cell Carcinoma. (2010)
  • Author(s): Nomura T, Murakami R, Toya R, Teshima K, Nakahara A, Hirai T, Hiraki A, Nakayama H, Yoshitake Y, Ota K, Obayashi T, Yamashita Y, Oya N, Shinohara M
  • Journal Title: Int J Radiat Oncol Biol Phys. Volume: 76 Pages: 1347-52
  • Peer Reviewed
• [Journal Article] Radiation-induced parotid gland changes in oral cancer patients : correlation between parotid volume and saliva production. (2010)
  • Author(s): Teshima K, Murakami R, Tomitaka E, Nomura T, Toya R, Hiraki A, Nakayama H, Hirai T, Shinohara M, Oya N, Yamashita Y.
  • Journal Title: Jpn J Clin Oncol. Volume: 40 Pages: 42-6
  • Peer Reviewed
• [Journal Article] 歯性感染症が原因で生じたと考えられた敗血症性肺塞栓症の1例 (2010)
  • Author(s): 吉田遼司、中山秀樹、永田将士、吉武義泰、手島慶子、篠原正徳
  • Journal Title: 日本口腔外科学会雑誌 Volume: 56 Pages: 49-53
  • NAID: 10027842203
  • Peer Reviewed
• [Journal Article] PTCH1遺伝子のミスセンス変異が認められた母斑基底細胞癌症候群の1例 (2010)
  • Author(s): 吉武義泰、中山秀樹、福間大喜、尾木秀直、平木昭光、篠原正徳
  • Journal Title: 日本口腔外科学会雑誌 Volume: 56 Pages: 40-44
  • NAID: 10027844265
  • Peer Reviewed
• [Journal Article] 8歳女児の下顎骨骨折に対して観血的整復固定術と保存的治療を併用した1例 (2010)
  • Author(s): 高宗康隆、中山秀樹、難波亜弥、福間大喜、田中拓也、永田将士、吉武義泰、尾木秀直、篠原正徳
  • Journal Title: 日本口腔顎顔面外傷学会誌 Volume: 9 Pages: 109-14
  • Peer Reviewed
• [Journal Article] Phase II Study of Preoperative Concurrent Chemoradiation Therapy with S-1 in Patients with T4 Oral Squamous Cell Carcinoma. (2010)
  • Author(s): Nomura T, Murakami R, Toya R, Teshima K, Nakahara A, Hirai T, Hiraki A, Nakayama H, Yoshitake Y, Ota K, Obayashi T, Yamashita Y, Oya N, Shinohara M
  • Journal Title: Int J Radiat Oncol Biol Phys. 76 Pages: 1347-1352
  • Peer Reviewed
• [Journal Article] Periodontal Status And Prevotella Intermedia Antibody In Acute Coronary Syndrome. (2009)
  • Author(s): Soejima H, Oe Y, Nakayama H, Matsuo K, Fukunaga T, Sugamura K, Kawano H, Sugiyama S, Shinohara M, Izumi Y, Ogawa H.
  • Journal Title: Int J Cardiol. 137(3) Pages: 304-306
  • Peer Reviewed
• [Journal Article] Significant Association Between Score of Periodontal Disease and Coronary Artery Disease Heart and Vessels (2009)
  • Author(s): Oe Y, Soejima H, Nakayama H, Fukunaga T, Sugamura K, Kawano H, Sugiyama S, Matsuo K, Shinohara M, Izumi Y, Ogawa H.
  • Journal Title: 24(2) Pages: 103-107
  • Peer Reviewed
• [Journal Article] Humanized anti-interleukin-6 receptor antibody suppresses tumor angiogenesis and in vivo growth of human oral squamous cell carcinoma. (2009)
  • Author(s): Shinriki S, Jono H, Ota K, Ueda M, Kudo M, Ota T, Oike Y, Endo M, Ibusuki M, Hiraki A, Nakayama H, Yoshitake Y, Shinohara M, Ando Y.
  • Journal Title: Clin Cancer Res. 15(17) Pages: 5426-5434
  • Peer Reviewed
• [Journal Article] Humanized anti-interleukin-6 receptor antibody suppresses tumor angiogenesis and in vivo growth of human oral squamous cell carcinoma. (2009)
  • Author(s): Shinriki S, Jono H, Ota K, Ueda M, Kudo M, Ota T, Oike Y, Endo M, Ibusuki M, Hiraki A, Nakayama H, Yoshitake Y, Shinohara M, Ando Y.
  • Journal Title: Clin Cancer Res. 15 Pages: 5426-5434
  • Peer Reviewed
• [Presentation] TS and cIAP2 are up-regulated in 5-FU-resistant oral squamous cell carcinoma cells (2010)
  • Author(s): Masashi Nagata, Hideki Nakayama, Yoshihiro Yoshitake, Daiki Fukuma, Takuya Tanaka, Ryoji Yoshida, Yasuo Takamune, Hidenao Ogi, Kenta Kawahara, Yoshihiro Nakagawa, Masanori Shinohara
  • Organizer: 第69回日本癌学会
  • Place of Presentation: グランキューブ大阪
• [Presentation] TS and cIAP2 are up-regulated in 5-FU-resistant oral squamous cells carcinoma cells (2010)
  • Author(s): Hideki Nakayama, Masashi Nagata, Yoshihiro Yoshitake, Daiki Fukuma, Tak uya Tanaka, Ryoji Yoshida, Yasuo Takamu ne, Hidenao Ogi, Kenta Kawahara, Yoshih iro Nakagawa, Masanori Shinohara
  • Organizer: 第51回大韓口腔顎顔面外科学会
  • Place of Presentation: 韓国 清州 ラマダプラザ清州ホテル
• [Presentation] TS and cIAP2 are up-regulated in 5-FU-resistant oral squamous cell carcinoma cells (2010)
  • Author(s): Hideki Nakayama, Masashi Nagata, Yoshihiro Yoshitake, Daiki Fukuma, Takuya Tanaka, Ryoji Yoshida, Yasuo Takamune, Hidenao Ogi, Kenta Kawahara, Yoshihiro Nakagawa, Masanori Shinohara
  • Organizer: 第51回大韓口腔顎顔面外科学会
  • Place of Presentation: 韓国清州ラマダプラザ清州ホテル
• [Presentation] 口腔癌症例における放射線30Gy照射および外科手術後の唾液量について (2010)
  • Author(s): 川原健太、吉田遼司、中山秀樹、永田将士、田中拓也、高宗康隆、吉武義泰、平木昭光、篠原正徳
  • Organizer: 第34回日本頭頸部癌学会
  • Place of Presentation: 東京京王プラザホテル
• [Presentation] TS and cIAP2 are up-regulated in 5-FU-resistant oral squamous cell carcinoma cells (2010)
  • Author(s): Masashi Nagata, Hideki Nakayama, Yoshihiro Yoshitake, Daiki Fukuma, Takuya Tanaka, Ryoji Yoshida, Yasuo Takamune, Hidenao Ogi, Kenta Kawahara, Yoshihiro Nakagawa, Masanori Shinohara
  • Organizer: 第69回日本癌学会
  • Place of Presentation: 大阪大阪国際会議場・リーガロイヤルホテル
• [Presentation] 顎口腔放線菌症患者の臨床的検討 (2010)
  • Author(s): 林田香織、中山秀樹、太田和俊、吉田遼司、野田彩、吉武義泰、尾木秀直、神力悟、平木昭光、篠原正徳
  • Organizer: 第55回日本口腔外科学会
  • Place of Presentation: 千葉幕張メッセ
• [Presentation] 口腔扁平上皮癌に対する化学放射線療法後の救済手術について (2010)
  • Author(s): 中山秀樹、篠原正徳
  • Organizer: 第28回日本口腔腫瘍学会総会
  • Place of Presentation: 学術総合センター(東京)
• [Presentation] 5-フルオロウラシル耐性口腔扁平上皮癌細胞の樹立とその細胞特性(Establishmentand characterization of 5-fluorouracil-resistant oral squamouscell carcinoma cell) (2009)
  • Author(s): 永田将士, 中山秀樹, 吉武義泰, 田中拓也, 吉田遼司, 福間大喜, 高宗康隆, 尾木秀直, 太田和俊, 平木昭光, 大林武久, 篠原正徳
  • Organizer: 第68回日本癌学会
  • Place of Presentation: パシフィコ横浜
• [Presentation] ヒト口腔扁平上皮癌細胞株を用いた5-FU耐性株の樹立と機能解析 (2009)
  • Author(s): 中山秀樹, 田中拓也, 吉武義泰, 高宗康隆, 福間大喜, 吉田遼司, 尾木秀直, 篠原正徳, 永田将士
  • Organizer: 第33回日本頭頸部癌学会
  • Place of Presentation: ロイトン札幌
• [Presentation] ヒト口腔扁平上皮癌細胞株を用いた5-FU耐性株の樹立と機能解析 (2009)
  • Author(s): 永田将士, 中山秀樹, 田中拓也, 吉武義泰, 高宗康隆, 福間大喜, 吉田遼司, 尾木秀直, 篠原正徳
  • Organizer: 第33回日本頭頸部癌学会総会
  • Place of Presentation: ロイトン札幌(北海道)
• [Presentation] 5-フルオロウラシル耐性口腔扁平上皮癌細胞の樹立とその細胞特性(Establishment and characterization of 5-fluorouracil-resistant oral squamous cell carcinoma cell) (2009)
  • Author(s): 永田将士, 中山秀樹, 吉武義泰, 田中拓也, 吉田遼司, 福間大喜, 高宗康隆, 尾木秀直, 太田和俊, 平木昭光, 大林武久, 篠原正徳
  • Organizer: 第68回日本癌学会総会
  • Place of Presentation: パシフィコ横浜(神奈川)
• [Remarks] ホームページ等
  • URL: http://www2.kuh.kumamoto-u.ac.jp/oralsurgery/