| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
The effects of benzophenanthridine alkaloids (QBA), known mainly as sanguinarine and chelerythrine, on the inhibition of cancer cell proliferation have been well established. Here, we demonstrated the antitumor activity of sanguinarine using in vitro assay systems in SAS, a human oral squamous cell carcinoma (OSCC) cell line. The anti-proliferative and -invasive effects were confirmed with IC_<50> values in the concentration range of 0.75-1.0 mM by MTT assay and invasive assay, respectively. Sanguinarine was also able to suppress cell anchorage-independent growth, whereas it did not affect the cells' adhering capabilities. Finally, we found that sanguinarine induces apoptotic cell death by activating caspase and the Bcl-2/Bax ratio. Taken together, these results demonstrate that sanguinarine,sodium pump (Na^+/K^+-ATPase) inhibitor, is a potential inhibitor of tumorigenesis and suggest that it may be valuable in the development of new anticancer drugs for the treatment of oral squamous cell carcinoma.
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