BRAK is a new type marker for development of tailor made medicine in head and neck squamous cell carcinoma.

• Project/Area Number: 21792037
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Kanagawa Dental College
• Principal Investigator: OZAWA Shigeyuki Kanagawa Dental College, 歯学部, 助教 (40434394)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ケモカイン / BRAK / 扁平上皮癌 / メチル化 / デシタビン / プロモーター / ゲフィチニブ

Research Abstract

BRAK is not expressed or negligible level in head and neck squamous cell carcinoma. We cleared that methylation of BRAK promoter regulates the expression of BRAK as a negative regulator.

Research Products

• [Journal Article] Reactive oxygen species (ROS) reduce the expression of BRAK/CXCL14 in human head and neck squamous cell carcinoma cells. (2010)
  • Author(s): Maehata Y, Ozawa S, Kobayashi K, Kato Y, Yoshino F, Miyamoto C, Izukuri K, Kubota E, Hata R, Lee MC.
  • Journal Title: Free Radio Res 44(8) Pages: 913-924
  • Peer Reviewed
• [Journal Article] Chemokine CXCL14/BRAK transgenic mice suppress growth of carcinoma cell transplants. (2010)
  • Author(s): Izukuri K, Suzuki K, Yajima N, Ozawa S, Ito S, Kubota E, Hata RI.
  • Journal Title: Transgenic Res
  • Peer Reviewed
• [Journal Article] Expression of a chemokine BRAK/CXCL14 in oral floor carcinoma cells reduces the settlement rate of the cells and suppresses their proliferation in vivo. (2010)
  • Author(s): Ito S, Ozawa S, Ikoma T, Yajima N, Kiyono T, Hata R.
  • Journal Title: Biomed Res. 31(3) Pages: 199-206
  • NAID: 130004903802
  • Peer Reviewed
• [Journal Article] Human p38 delta MAP kinase mediates UV irradiation induced up-regulation of the gene expression of chemokine BRAK/CXCL14. (2010)
  • Author(s): Ozawa S, Ito S, Kato Y, Kubota E, Hata R.
  • Journal Title: Biochem Biophys Res Commun. 396(4) Pages: 1060-1064
  • Peer Reviewed
• [Journal Article] Functional characterization of proximal promoter of gene for human BRAK/CXCL14, a tumor-suppressing chemokine. (2010)
  • Author(s): Komori R, Ozawa S, Kato Y, Shinji H, Kimoto S, Hata R.
  • Journal Title: Biomed Res. 31(2) Pages: 123-131
  • NAID: 130004903789
  • Peer Reviewed
• [Journal Article] Expression of tumour-suppressing chemokine BRAK/CXCL14 reduces cell migration rate of HSC-3 tongue carcinoma cells and stimulates attachment to collagen and formation of elongated focal adhesions in vitro. (2010)
  • Author(s): Sato K, Ozawa S, Izukuri K, Kato Y, Hata R.
  • Journal Title: Cell Biol Int. 34(5) Pages: 513-522
  • Peer Reviewed
• [Journal Article] Human p38 delta MAP kinase mediates UV irradiation induced up-regulation of the gene expression of chemokine BRAK/CXCL14. (2010)
  • Author(s): Ozawa S, Ito S, Kato Y, Kubota E, Hata R.
  • Journal Title: Biochem Biophys Res Commun Volume: 396(4) Pages: 1060-4
  • Peer Reviewed
• [Journal Article] BRAK/CXCL14 expression in oral carcinoma cells completely suppresses tumor cell xenografts in SCID mouse. (2009)
  • Author(s): Ozawa S, Kato Y, Kubota E, Hata R.
  • Journal Title: Biomed Res. 30(5) Pages: 315-318
  • NAID: 130004903768
  • Peer Reviewed
• [Journal Article] Restoration of BRAK / CXCL14 gene expression by gefitinib is associated with antitumor efficacy of the drug in head and neck squamous cell carcinoma. (2009)
  • Author(s): Ozawa S, Kato Y, Ito S, Komori R, Shiiki N, Tsukinoki K, Ozono S, Maehata Y, Taguchi T, Imagawa-Ishiguro Y, Tsukuda M, Kubota E, Hata R.
  • Journal Title: Cancer Sol. 100(11) Pages: 2202-2209
  • NAID: 10027197383
  • Peer Reviewed
• [Journal Article] BRAK/CXCL14 expression in oral carcinoma cells completely suppresses tumor cell xenografts in SCID mouse (2009)
  • Author(s): Ozawa S, Kato Y, Kubota E, Hata R.
  • Journal Title: Biomed Res. 30 Pages: 315-318
  • NAID: 130004903768
  • Peer Reviewed
• [Journal Article] Restoration of BRAK / CXCL14 gene expression by gefitinib is associated with antitumor efficacy of the drug in head and neck squamous cell carcinoma. (2009)
  • Author(s): Ozawa S, Kato Y, Ito S, Komori R, Shiiki N, Tsukinoki K, Ozono S, Maehata Y, Taguchi T, Imagawa-Ishiguro Y, Tsukuda M, Kubota E, Hata R.
  • Journal Title: Cancer Sci. 100 Pages: 2202-2209
  • NAID: 10027197383
  • Peer Reviewed
• [Presentation] Oxidative stress reduce expression of CXCL14/BRAK in human cells (2011)
  • Author(s): MAEHATA Y, OZAWA S, MIYAMOTO C, KOBAYASHI K, KOMORI R, HATA R.-I, LEE M.C
  • Organizer: IADR General Session
  • Place of Presentation: San Diego
• [Presentation] Analysis of promoter region of huma BRAK/CXCL14 in SCC cells. (2011)
  • Author(s): KOMORI R, OZAWA S, KATO Y, MAEHATA Y, SHINJI H, HATA R.-I, KIMOTO S
  • Organizer: IADR General Session
  • Place of Presentation: San Diego
• [Presentation] BRAK発現制御機構と口腔扁平上皮癌に対する抗腫瘍効果についての基礎的研究 (2010)
  • Author(s): 小澤重幸
  • Organizer: 日本口腔外科学会総会
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2010-10-17
• [Presentation] Chemokine CXCL14/BRAK transgenic mice suppress growth and metastasis of carcinoma cells. (2010)
  • Author(s): Hata R, Izukuri K, Suzuki K, Kato Y, Yajima N, Ozawa S, Ito S, Kubota E
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-23
• [Presentation] Chemokine BRAK/CXCL14 is a multifunctional tumor suppressor. (2010)
  • Author(s): Hata R, Izukuri K, Suzuki K, Yajima N, Ozawa S, Ito S, Kubota E
  • Organizer: 88^<th> General Session & Exhibition of the IADR・5^<th> General Session of the Pan European Region of the IADR
  • Place of Presentation: Barcelona
  • Year and Date: 2010-07-14
• [Presentation] Gene expression signatures at acidic extracellular pH in metastatic melanoma cells. (2010)
  • Author(s): Kato Y, Ozawa S, Maehata Y, Maeda T, Kubota E, Tsukuda M, Miyazaki K, Hata R
  • Organizer: Joint MRS-AACR Conference. Metastasis and the Tumor Microenvironment
  • Place of Presentation: Philadelphia
• [Presentation] Suppression of growth of Lewis lung carcinoma cell xenografts in BRAK transgenic mouse (2009)
  • Author(s): Kazuhito Izukuri, Kenji Suzuki, Shigeyuki Ozawa, Eiro Kubota, Ryu-Ichiro Hata
  • Organizer: Production of cancer resistant mouse. Yokosuka Science Festa
  • Place of Presentation: Shonan Village Center, Hayama
  • Year and Date: 2009-07-06
• [Presentation] Chemokine BRAK stimulates apoptosis elicited by gefitinib in oral squamous cell carcinoma (2009)
  • Author(s): Shin Ito, Shigeyuki Ozawa, Naoto Shiiki, Keiichi Tsukinoki, Eiro Kubota, Yasumasa Kato, Takahide Taguchi, Yukari Imagawa-Ishiguro, Mamoru Tsukuda, Ryu-Ichiro Hata
  • Organizer: Yokosuka Science Festa
  • Place of Presentation: Shonan Village Center, Hayama
  • Year and Date: 2009-07-06
• [Presentation] Expression of BRAK/CXCL14 is associated with antitumor efficacy of gefitinib in head and neck squamous cell carcinoma. (2009)
  • Author(s): Shigeyuki Ozawa, Yasumasa Kato, Shin Ito, Reika Komori, Kenji Suzuki, Keiichi Tsukinoki, Yojiro Maehata, Takahide Taguchi, Yukari Imagawa-Ishiguro, Mamoru Tsukuda, Eiro Kubota, Ryu-Ichiro Hata
  • Organizer: Yokosuka Science Festa
  • Place of Presentation: Shonan Village Center, Hayama
  • Year and Date: 2009-07-06
• [Presentation] Functional analysis of promoter region of humanBRAK/CXCL14 (2009)
  • Author(s): Reika Komori, Shigeyuki Ozawa, Yasumasa Kato, Hisaaki Shinji, Shigenari Kimoto, Ryu-Ichiro Hata
  • Organizer: a tumor progression suppressor. Yokosuka Science Festa
  • Place of Presentation: Shonan Village Center, Hayama
  • Year and Date: 2009-07-06
• [Presentation] Reactive oxygen species reduce the expression of BRAK/CXCL14 in human head and neck squamous cell carcinoma cells. (2009)
  • Author(s): Yojiro Maehata, Shigeyuki Ozawa, Chihiro Miyamoto, Kyo Kobayashi, Yasumasa Kato, Fumihiko Yoshino, Masaichi-Chang-il Lee, Ryu-Ichirc Hata
  • Organizer: Yokosuka Science Festa
  • Place of Presentation: Shonan Village Center, Hayama
  • Year and Date: 2009-07-05
• [Presentation] Functional Analysis of Promoter of Human BRAK/CXCL14, A Tumor Suppressor. (2009)
  • Author(s): Reika Komori, Shigeyuki Ozawa, Yasumasa Kato, Hisaaki Shinji, Shigenari Kimoto, Ryu-Ichiro Hata
  • Organizer: 87^<th> General Session & Exhibition of the IADR・38^<th> Annual Meeting of the AADR・33^<rd> Annual Meeting of the CADR
  • Place of Presentation: Miami Beach Convention Center, Miami, Florida
• [Presentation] A new mechanism of tumor suppression by gefitinib in HNSCC. (2009)
  • Author(s): Shigeyuki Ozawa, Yasumasa Kato, Shin Ito, Reika Komori, Naoto Shiiki, Keiichi Tukinoki, Satoru Ozono, Yojiro Maehata, Takahide Taguchi, Yukari Imagawa-Ishiguro, Mamoru Tsukuda, Eiro Kubota, Ryu-Ichiro Hata
  • Organizer: 87^<th> General Session & Exhibition of the IADR・38^<th> Annual Meeting of the AADR・33^<rd> Annual Meeting of the CADR
  • Place of Presentation: Miami Beach Convention Center, Miami, Florida
• [Presentation] Expression of BRAK/CXCL14 is associated with antitumor efficacy of gefitinib in head and neck squamous cell carcinoma (2009)
  • Author(s): Ryu-Ichiro Hata, Shigeyuki Ozawa, Yasumasa Kato, Shin Ito, Reika Komori, Naoto Shiiki, Keiichi Tsukinoki, Yojiro Maehata, Eiro Kubota
  • Organizer: ECCO15 and 34^<th> ESMO Multidisciplinary Congress
  • Place of Presentation: Berlin
• [Presentation] BRAK/CXCL14 transgenic mice suppress growth of tumor cell xenografts. (2009)
  • Author(s): Ryu-Ichiro Hata, Kazuhito Izukuri, Kenji Suzuki, Nobuyuki Yajima, Shin Ito, Shigeyuki Ozawa, Eiro Kubota
  • Place of Presentation: Boston
• [Presentation] EGF受容体阻害剤とデシタビンを用いた併用療法の基礎的研究 (2009)
  • Author(s): 小澤重幸, 鈴木健司, 生駒丈晴, 久保田英朗
  • Organizer: 第54回日本口腔外科学会総会
  • Place of Presentation: 札幌コンベンションセンター(北海道)