| Project/Area Number |
21792057
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
KUBOTA Mamoru Tohoku University, 大学院・歯学研究科, 大学院非常勤講師 (30374945)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | アポトーシス / 歯の移動 / シグナル伝達 / 歯周組織 / 細胞死 / 歯学 / 細胞・組織 |
|---|
| Research Abstract |
The purpose of this study is to elucidate the mechanism of site-specific periodontium remodeling during orthodontic tooth movement. Therefore it was paid attention the signal that the cells, which constituted periodontium, released after death, and a study was conducted. The maxillary first molars of 8 weeks-old male Wistar rats were moved, and apoptosis was observed morphologically staining by the TUNEL methods. Furthermore, tooth movement was examined in the presence of Z-VAD, which was a repressor of caspase. In addition, osteoblasts and a periodontal ligament cells were cultured in vitro, and apoptosis was induced using low-serum culture media, hypoxia or continuous compression force condition. The survival rate of the cell decreased, and some signal transduction pathways were detected as candidates by gene expression pattern analysis.
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