Exploring the way to make a correct induction from stem cells to odontoblast and ameloblasts.

• Project/Area Number: 21792072
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Okayama University
• Principal Investigator: KUROSAKA Hiroshi Okayama University, 大学院・医歯薬学総合研究科, 助教 (20509369)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: 歯 / 再生 / ES細胞 / 歯原性間葉細胞 / 位置情報 / 発生 / 幹細胞

Research Abstract

We found that reconstructed artificial tooth can develop when proper cell ratio of mesenchymal cells and ES cells supplied. Also we found the way to induce odontblasts from stem cells which exists in rat dental pulp.

Research Products

• [Journal Article] Isolation of multipotent stem cells in human periodontal ligament using stage-specific embryonic antigen-4. (2010)
  • Author(s): Kawanabe N, Murata S, Murakami K, Ishihara Y, Hayano S, Kurosaka H, Kamioka H, Takano-Yamamoto T, Yamashiro T
  • Journal Title: Differentiation 79(2)(Epub 2009 Nov 27) Pages: 74-83
• [Journal Article] Isolation of multipotent stem cells in human periodontal ligament using stage-specific embryonic antigen-4 (2009)
  • Author(s): Kawanabe N
  • Journal Title: Differentiation 79 Pages: 7483-7483
  • Peer Reviewed
• [Presentation] Exploring the roles of core-binding factor β (CBFB) in tooth development and ameloblast physiology. (2010)
  • Author(s): Hiroshi Kurosaka, Satoru Hayano, Koh-ichi Kuremoto, Hidemitsu Harada, Islam Nurul, Ichiro Taniuchi, Takashi Yamashiro.
  • Organizer: The 17th international RUNX workshop
  • Place of Presentation: Hiroshima, Japan
• [Presentation] Exploring the roles of core-binding factor β (CBFB) in tooth development and ameloblast physiology. (2010)
  • Author(s): Hiroshi Kurosaka, Satoru Hayano, Koh-ichi Kuremoto, Hidemitsu Harada, Islam Nurul, Ichiro Taniuchi, Takashi Yamashiro.
  • Organizer: Gordon conference Craniofacial Morphogenesis & Tissue Regeneration
  • Place of Presentation: Lucca, Barga
• [Presentation] The role of heparan sulfate 6-O-endosulfatases Sulf1 and Sulf2 during tooth development. (2010)
  • Author(s): Satoru Hayano, Hiroshi Kurosaka, Ina Kalus, Thomas Dierks, Takashi Yamashiro.
  • Organizer: Gordon conference Craniofacial Morphogenesis & Tissue Regeneration
  • Place of Presentation: Lucca, Barga
• [Presentation] Exploring the roles of core-binding factor β (CBFB) in tooth development and_ameloblast physiology (2010)
  • Author(s): Hiroshi Kurosaka, et al.
  • Organizer: Gordon conference Craniofacial Morphogenesis & Tissue Regeneration
  • Place of Presentation: Lucca, Barga
• [Presentation] Exploring the roles of core-binding factor β (CBFB) in tooth development and ameloblast physiology (2010)
  • Author(s): Hiroshi Kurosaka, et al.
  • Organizer: The 17th international RUNX workshop
  • Place of Presentation: Hiroshima, Japan
• [Presentation] 象牙質再生の基盤研究 (2009)
  • Author(s): 黒坂寛
  • Organizer: 機能再生・再発はセンター科学専攻系ミニシンポジウム
  • Place of Presentation: 岡山市
  • Year and Date: 2009-12-15
• [Presentation] The role of heparan sulfate 6-O-endosulfatases Sulf1 and Sulf2 during tooth development (2009)
  • Author(s): Satoru Hayano, Hiroshi Kurosaka, Ina Kalus, Thomas Dierks, Takashi Yamashiro.
  • Organizer: Gordon conference Bones & Teeth.
  • Place of Presentation: Biddeford, ME
• [Presentation] The role of heparan sulfate 6-0-endosulfatases Sulfl and Sulf2 during tooth development (2009)
  • Author(s): Hayano S
  • Organizer: Gordon Research Conferences Bones & Teeth
  • Place of Presentation: Biddeford, ME
• [Patent(Industrial Property Rights)] 歯髄細胞から象牙芽細胞への分化誘導方法 (2009)
  • Inventor(s): 山城隆、川邊紀章、黒坂寛
  • Industrial Property Rights Holder: 山城隆、川邊紀章、黒坂寛
  • Filing Date: 2009-11-17
• [Patent(Industrial Property Rights)] 歯髄細胞から象牙芽細胞への分化誘導方法 (2009)
  • Inventor(s): 山城隆, 川邉紀章, 黒坂寛
  • Industrial Property Rights Holder: 山城隆, 川邉紀章, 黒坂寛
  • Industrial Property Number: 2009-262378
  • Filing Date: 2009-11-17