Regulation of growth-plate chondrocyte differentiation by self-produced secreted and membrane proteins
Project/Area Number |
21792086
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Orthodontic/Pediatric dentistry
|
Research Institution | Nagasaki University |
Principal Investigator |
HIDAKA Kiyoshi 長崎大学, 大学院・医歯薬学総合研究科, 助教 (10389421)
|
Co-Investigator(Renkei-kenkyūsha) |
YAMAMOTO Masaya 福島県立医科大学, 医学部, 准教授 (20446115)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 軟骨細胞 / 軟骨細胞分化 / 成長軟骨 / 分泌蛋白質 / SST-REX法 / 膜蛋白質 |
Research Abstract |
This is the study to screen proteins with signal sequence at their N-terminus produced by growth-plate chondrocytes to find key molecules to regulate chondrocyte differentiation by autocrine/ paracrine mechanisms. More than 70 of secreted and membrane proteins were identified by SST-REX method. We focused on secreted protein IGFBP5 and membrane protein FGFR5, and found that these proteins were expressed at specific stage during the differentiation in vivo and in vitro.
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Report
(4 results)
Research Products
(27 results)