| Project/Area Number |
21792090
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Kagoshima University |
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Principal Investigator |
YAMAMOTO Yoshitake Kagoshima University, 医歯学総合研究科, 助教 (50380465)
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| Research Collaborator |
MITSUI Kaoru 鹿児島大学, 医歯学総合研究科, 講師 (40324975)
KOSAI Ken-Ichiro 鹿児島大学, 医歯学総合研究科, 教授 (90258418)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 骨芽細胞 / 細胞増殖 / 細胞分化 / 分化・増殖 / 骨改造 |
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| Research Abstract |
To date, there have been no clear reports that Msx2 take part in the mechanism of osteoblast differentiation, which is responsible for the bone remodeling and regeneration in orthodontic treatments. The purpose of the present study, therefore, was to investigate the roles of Msx2 in osteoblast differentiation and bone formation with mouse Embryonic Stem cells. ES cells which transfected with Msx2 were cocultured with feeder cells, and alkaline phosphatase activity and mRNA levels of osteoblastic phenotype were determined for cell differentiation. The value of bone nodules was also determined for mineralization. As a result, it was suggested that Msx2 play a key role in controlling osteoblast differentiation.
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