Identification of am3 gene and an underlying study for establishment of risk diagnostic procedure using SNPs

• Project/Area Number: 21792102
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Tsurumi University
• Principal Investigator: NARIYAMA Megumi Tsurumi University, 歯学部, 助教 (90440304)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 分子遺伝学 / 歯の発育異常 / am3遺伝子 / リスク診断 / ELマウス

Research Abstract

The present study attempted to define genes causing an absence of the third molars in EL mice with the majority exhibiting the absence of all third molars. I analyzed the mRNA expressions of candidate genes (Lef1, Egf) in the am3 locus indicated with linkage analysis and other candidate genes (Shh, Pitx2, Bmp2, Bmp4) in the EL and control mice using Real-time PCR. The results of PCR suggest that Lef1, Egf, Bmp2, and Bmp4 are involved in the absence of the third molars in EL mice.

Research Products

• [Journal Article] Effect of third molar absence on development of masticatory muscles inEL mice. (2010)
  • Author(s): Megumi Nariyama, Yuki Kota, Itaru Tashima, Tadafumi Tomohiro, Akira Yamane, Yoshinobu Asada
  • Journal Title: Pediatric Dental Journal Vol.20, No.2 Pages: 122-129
  • Peer Reviewed
• [Journal Article] Effect of third molar absence on development of masticatory muscles in EL mice. (2010)
  • Author(s): Megumi Nariyama, Yuki Kota, Itaru Tashima, Tadafumi Tomohiro, Akira Yamane, Yoshinobu Asada
  • Journal Title: Pediatric Dental Journal Volume: 20 Pages: 122-129
  • NAID: 10027485798
  • Peer Reviewed
• [Journal Article] Effect of third molar absence on development of masticatory muscles inEL mice. (2010)
  • Author(s): Meeumi Nariyama, Yuki Kota,Itaru Tashima, Tadafumi Tomohiro, Akira Yamane, Yoshiuobu Asada
  • Journal Title: Pediatric Dental Journal 20巻(In press)
  • Peer Reviewed
• [Presentation] Lack of teeth and expression of myosins in masticatory muscles (2010)
  • Author(s): Yamane A, Nariyama M, Kota Y, Asada Y
  • Organizer: 58th annual meeting of JADR
  • Place of Presentation: Kyushu Dental College, Kitakyushu
  • Year and Date: 2010-11-20
• [Presentation] 咬筋の神経筋接合部におけるアセチルコリン受容体クラスター形成 (2010)
  • Author(s): 森愛美, 成山明具美, 阿保徳寿, 山根明, 朝田芳信
  • Organizer: 平成22年度、一般社団法人日本小児歯科学会秋季大会
  • Place of Presentation: 郡山市民文化センター
• [Presentation] Effects of Lack of Occlusal Activity on Masticatory Myogenesis (2010)
  • Author(s): Nariyama M, Kota Y, Yamane A, Tomohiro T, Asada Y
  • Organizer: 88th General Session & Exhibition of the IADR
  • Place of Presentation: Centre de Convencions Internacional Barcelona
• [Presentation] マウス第三臼歯欠如が咀嚼筋の発達過程に及ぼす影響 (2010)
  • Author(s): 成山明具美, 古宇田ゆき, 田島格, 友廣忠文, 山根明, 朝田芳信
  • Organizer: 第48回日本小児歯科学会大会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] Effects of Lack of Occlusal Activity on Masticatory Myogenesis (2010)
  • Author(s): Nariyama M, Kota Y, Yamane A, Tomohiro T, Asada Y
  • Organizer: 88th General Session & Exhibition of the IADR
  • Place of Presentation: Centre de Convencions Internacional Barcelona, Spain
• [Presentation] 当センターの地域障害者歯科医療への役割と来院患者の26年間の変化 (2009)
  • Author(s): 中島智仁, 山本俊雄, 端山智弘, 伊奈幹晃, 倉本弘樹, 朝生博行, 池村雄介, 荒木信清, 成山明具美, 小平裕恵, 阿倍佳子, 關田俊介
  • Organizer: 第26回 日本障害者歯科学会総会および学術大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-10-31