| Project/Area Number |
21792130
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Periodontal dentistry
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| Research Institution | Showa University |
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Principal Investigator |
TAKADA Takatora Showa University, 歯学部, 普通研究生 (20384323)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | SIRT1 / 骨代謝 / 歯周組織 / Sirt / 破骨細胞 |
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| Research Abstract |
Sirt1 is known as regulator of the aging process. We examined a function of Sirt1 in gingival epithelial cells and osteoclasts which constituted periodontium. In a gingival epithelia cell, LPS increased the levels of Sirt1. We examined the role of Sirt1 for the production of TNF-α by LPS in gingival epithelial cells. Although LPS increased TNF-α and NF-kB activation, sirtinol, sirt1 inhibitor prevented LPS-induced TNF-α. On the other hand, we treated resveratrol, Sirt1 activator in osteoclast formation assay to elucidate a function of Sirt1 in osteoclatogenesis. Resveratrol increased the number of RANKL-induced osteoclasts. We investigated osteoclast-related gene expression using microarray system to clarify whether activation of Sirt1 controlled the osteoclast formation. The levels of cathepsin K and NFATc1 gene expression were significantly decreased.
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