Development of infection mechanism of culture-negative endocarditis

Research Project

Project/Area Number	21792155
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Social dentistry
Research Institution	Kagoshima University
Principal Investigator	SOUTOME Sakiko 鹿児島大学, 医学部・歯学部附属病院, 助教 (20325799)
Co-Investigator(Kenkyū-buntansha)	YAMAGUCHI Taihei 鹿児島大学, 医歯学総合研究科, 准教授 (80230358) OHO Takahiko 鹿児島大学, 医歯学総合研究科, 教授 (50160940)
Project Period (FY)	2009 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords	レンサ球菌 / フィブロネクチン / モノクローナル抗体 / 心内膜炎 / Granulicatella adiacens
Research Abstract	Granulicatella adiacens is a member of the oral microbiota, formerly known as nutritionally variant streptococci, and is often isolated from endocarditis patients. In the present study we identified a surface protein, designated Cha, which binds to fibronectin, by a plaque hybridization procedure using the cshA sequence as probe, which encodes a fibronectin-binding molecule of Streptococcus gordonii DL1. The cha sequence was highly homologous to cshA and encoded a product of 2351 amino acid residues. The protein comprised a unique sequence in the N-terminal half region. The C-terminal region contained nine complete, and one incomplete, 115-amino acid residue repeat blocks. Among eight strains of nutritionally variant streptococci, three G. adiacens strains and one Abiotrophia defectiva strain carried the cha gene.