| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Research Abstract |
In this study, we examined a hypothesis that modulation of histone acetylation or methylation may also have some effect on the osteoclast differentiation. RANKL treatment induced the number of multinucleated osteoclast-like cells and the TRAP activities. Both histone deacetylase and methyltransferase inhibiton decreased the number of RANKL-induced osteoclast-like cells and TRAP activities in a dose-dependent manner. During the experiments, we also observed that RANKL treatment induced Raw264.7 cell death and the histone-deacetylase or histone methyltransferase-inhibitor dose-dependently suppressed the RANKL-induced cell death.
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