Study of oral biofilm inhibitors from edible mushroom

• Project/Area Number: 21792165
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Social dentistry
• Research Institution: Iwate Biotechnology Research Center
• Principal Investigator: YANO Akira Iwate Biotechnology Research Center, 生物資源研究部, 主席研究員 (50312286)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 予防歯科学 / Lentinula edodes / Streptococcus mutans / two-component system / glucosyltransferase / biofilm / シイタケ / Streptococcus sobrinus / Biofilm

Research Abstract

We found the inhibitory effects of water-soluble extracts from edible mushroom, especially "Lentinula edodes", on sucrose-dependent biofilm formation by Streptococcus mutans . The extracts inhibit the water-soluble glucan synthesis by glucosyltransferase (GTF), expression of gtf genes and signaling genes related to the biofilm formation. We found the strong suppression of the quorum-sensing peptide gene, comC.

Research Products

• [Journal Article] The inhibitory effects of mushroom extracts on sucrose-dependent oral biofilm formation. (2010)
  • Author(s): Yano A, Kikuchi S, Yamashita Y, Sakamoto Y, Nakagawa Y, Yoshida Y.
  • Journal Title: Appl Microbiol Biotechnol 86 Pages: 615-623
  • Peer Reviewed
• [Journal Article] The inhibitory effects of mushroom extracts on sucrose-dependent oral biofilm formation (2010)
  • Author(s): Yano A, Kikuchi S, Yamashita Y, Sakamoto Y, Nakagawa Y, Yoshida Y
  • Journal Title: Applied Microbiology and Biotechnology 86 Pages: 615-623
  • Peer Reviewed
• [Journal Article] Secretory expression of the non-secretory-type Lentinula edodes laccase by Aspergillus oryzae (2009)
  • Author(s): Yano A, Kikushi S, Nakagawa Y, Sakamoto Y, Sato T
  • Journal Title: Microbiological Research 164 Pages: 642-649
  • Peer Reviewed
• [Presentation] シイタケ抽出成分によるミュータンスレンサ球菌のバイオフィルム形成阻害機構の解析 (2011)
  • Author(s): 矢野明、菊地明香、山田秀俊
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都
• [Presentation] シイタケ抽出成分によるミュータンスレンサ球菌のバイオフィルム形成阻害機構の解析 (2011)
  • Author(s): 矢野明、菊地明香、山田秀俊
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: (震災のため大会要旨集発送にて大会成立)
• [Presentation] 担子菌抽出成分の口腔バイオフィルム形成阻害効果 (2010)
  • Author(s): 矢野明、菊地明香、金野尚武、坂本裕一、吉田康夫
  • Organizer: 日本農芸化学会 2010年度大会
  • Place of Presentation: 東京(東大)
  • Year and Date: 2010-03-29
• [Presentation] 担子菌抽出成分の口腔バイオフィルム形成阻害効果 (2010)
  • Author(s): 矢野明、菊地明香、斎藤絵里奈、金野尚武、坂本裕一
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 東京
• [Presentation] The water-soluble mushroom extracts perturb the biofilm formation. (2010)
  • Author(s): Yano A, Kikuchi S, Yoshida Y.
  • Organizer: 58th Annual Meeting, Japanese association for Dental Research
  • Place of Presentation: Kitakyushu
• [Presentation] The water soluble mushroom extracts perturb the biofilm formation. (2010)
  • Author(s): Yano A, Kikuchi S, Yoshida Y
  • Organizer: 58^<th> Annual Meeting, Japanese association for Dental Research
  • Place of Presentation: Kyusyu Dental College, Kitakyusyu, Japan
• [Presentation] The inhibitory effects of mushroom extracts on oral biofilm formation (2009)
  • Author(s): Yano A, Yoshida Y, Kikuchi S, Sakamoto Y, Nakagawa Y, Kato H
  • Organizer: International Association for Dental Research(87^<th> General Session & Exhibition)
  • Place of Presentation: Miami, Florida, USA
  • Year and Date: 2009-04-04
• [Presentation] The inhibitory effects of mushroom extracts on oral biofilm formation. (2009)
  • Author(s): Yano A, Yoshida Y, Kikuchi S, Sakamoto Y, Nakagawa Y, Kato H.
  • Organizer: 87th General Session & Exhibition of the International Association for Dental Research.
  • Place of Presentation: Miami, Florida
• [Remarks]
  • URL: http://www.ibrc.or.jp/