Preparation of chitosan microspheres for the medical application

Research Project

Project/Area Number	21800035
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Biomedical engineering/Biological material science
Research Institution	Okayama University
Principal Investigator	SHIROSAKI Yuki Okayama University, 大学院・自然科学研究科, 助教 (40533956)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥2,678,000 (Direct Cost: ¥2,060,000、Indirect Cost: ¥618,000) Fiscal Year 2010: ¥1,274,000 (Direct Cost: ¥980,000、Indirect Cost: ¥294,000) Fiscal Year 2009: ¥1,404,000 (Direct Cost: ¥1,080,000、Indirect Cost: ¥324,000)
Keywords	キトサン / ミクロ粒子 / ミクロカプセル / 薬物徐放 / 細胞適合性 / 有機-無機複合体 / 骨形成
Research Abstract	In this study, Porous particles and capsules were prepared to recover of the damaged human tissues. Chitosan and γ-glycidoxypropyltrymethoxysilane (GPTMS) were used as the starting materials. Porous particles were prepared by dropping precursor sol with CaCl_2 or without into liquid nitrogen. Obtained porous particles had about 2 mm in diameter and 10-30μm in pore size. HAp particles of about 1μm in diameter deposited on the pore surface by soaking thus obtained hybrid parties in 0.01 M Na2HPO4 solution at 80℃ for 7 days. Bovine serum albumin (BSA) adsorbed much on the chitosan-GPTMS particles. Capsules were prepared by dropping carboxymethylcellulose into chitosan-GPTMS sols. The releasing of bioactive molecules included into the capsules was controlled by the cross-linking degree. Moreover the capsules did not show the cytotoxicity.