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The effects on skeletal muscles in medaka of the various muscle activities ; Can medaka be a model organism for human skeletal muscle researches?

Research Project

Project/Area Number 21800096
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Sports science
Research InstitutionJapan Aerospace Exploration Agency

Principal Investigator

MASAHIRO Terada  Japan Aerospace Exploration Agency, 有人宇宙環境利用ミッション本部, 宇宙航空プロジェクト研究員 (10553422)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥2,639,000 (Direct Cost: ¥2,030,000、Indirect Cost: ¥609,000)
Fiscal Year 2010: ¥1,248,000 (Direct Cost: ¥960,000、Indirect Cost: ¥288,000)
Fiscal Year 2009: ¥1,391,000 (Direct Cost: ¥1,070,000、Indirect Cost: ¥321,000)
Keywordsメダカ / 骨格筋 / 宇宙 / レーザーマイクロダイセクション
Research Abstract

In this study, it is aimed to examine whether medaka can be studied as a model organism for skeletal muscle fiber atrophy in astronauts who stayed in space for long time. Therefore, we observed how the skeletal muscle of medaka was effected by the change of the muscle activity. The frozen cross-seciton of medaka was cut. Then, we detected the slow and/or fast skeletal muscle by the immunohistochemical staining. Moreover, by Laser Micro-Dissection (LMD) method, the slow and fast skeletal muscle fibers were dissected from the frozen cross-sections. And we analyzed the expression of the ubiquitin ligase by real-time RT-PCR.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (6 results)

All 2010

All Presentation (6 results)

  • [Presentation] メダカを対象とした骨格筋解析ならびに心電図解析2010

    • Author(s)
      寺田昌弘
    • Organizer
      第56回日本宇宙航空環境医学会大会
    • Place of Presentation
      所沢市。
    • Year and Date
      2010-11-12
    • Related Report
      2010 Final Research Report
  • [Presentation] メダカを対象とした骨格筋解析ならびに心電図解析2010

    • Author(s)
      寺田昌弘
    • Organizer
      第56回日本宇宙航空環境医学会大会
    • Place of Presentation
      所沢市
    • Year and Date
      2010-11-12
    • Related Report
      2010 Annual Research Report
  • [Presentation] メダカ骨格筋の評価法の検討;メダカ骨格筋は宇宙で萎縮するのか?2010

    • Author(s)
      寺田昌弘
    • Organizer
      日本宇宙生物科学会第24回大会
    • Place of Presentation
      仙台市。
    • Year and Date
      2010-09-17
    • Related Report
      2010 Final Research Report
  • [Presentation] メダカ骨格筋の評価法の検討;メダカ骨格筋は宇宙で萎縮するのか2010

    • Author(s)
      寺田昌弘
    • Organizer
      日本宇宙生物科学会第24回大会
    • Place of Presentation
      仙台市
    • Year and Date
      2010-09-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] メダカ骨格筋を対象とした評価法の検討2010

    • Author(s)
      寺田昌弘
    • Organizer
      第18回日本運動生理学会大会
    • Place of Presentation
      鹿児島市。
    • Year and Date
      2010-07-31
    • Related Report
      2010 Final Research Report
  • [Presentation] メダカ骨格筋を対象とした評価法の検討2010

    • Author(s)
      寺田昌弘
    • Organizer
      第18回日本運動生理学会大会
    • Place of Presentation
      鹿児島市
    • Year and Date
      2010-07-30
    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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