| Project/Area Number |
21800096
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Sports science
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| Research Institution | Japan Aerospace Exploration Agency |
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Principal Investigator |
MASAHIRO Terada Japan Aerospace Exploration Agency, 有人宇宙環境利用ミッション本部, 宇宙航空プロジェクト研究員 (10553422)
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|---|
| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥2,639,000 (Direct Cost: ¥2,030,000、Indirect Cost: ¥609,000)
Fiscal Year 2010: ¥1,248,000 (Direct Cost: ¥960,000、Indirect Cost: ¥288,000)
Fiscal Year 2009: ¥1,391,000 (Direct Cost: ¥1,070,000、Indirect Cost: ¥321,000)
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| Keywords | メダカ / 骨格筋 / 宇宙 / レーザーマイクロダイセクション |
|---|
| Research Abstract |
In this study, it is aimed to examine whether medaka can be studied as a model organism for skeletal muscle fiber atrophy in astronauts who stayed in space for long time. Therefore, we observed how the skeletal muscle of medaka was effected by the change of the muscle activity. The frozen cross-seciton of medaka was cut. Then, we detected the slow and/or fast skeletal muscle by the immunohistochemical staining. Moreover, by Laser Micro-Dissection (LMD) method, the slow and fast skeletal muscle fibers were dissected from the frozen cross-sections. And we analyzed the expression of the ubiquitin ligase by real-time RT-PCR.
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