Reliability evaluation of fluorescence probe method for molecular diffusion measurement in lipid bilayer by atomic force microscopy
| Project/Area Number |
21810009
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Nanomaterials/Nanobioscience
|
|---|
| Research Institution | Kanazawa University |
|---|
Principal Investigator |
ASAKAWA Hitoshi Kanazawa University, バイオAFM先端研究センター, 助教 (90509605)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥2,678,000 (Direct Cost: ¥2,060,000、Indirect Cost: ¥618,000)
Fiscal Year 2010: ¥1,274,000 (Direct Cost: ¥980,000、Indirect Cost: ¥294,000)
Fiscal Year 2009: ¥1,404,000 (Direct Cost: ¥1,080,000、Indirect Cost: ¥324,000)
|
|---|
| Keywords | 原子間力顕微鏡 / ナノテクノロジー / 生体膜 / 脂質二重層 / 脂質分子拡散 / 蛍光プローブ標識 |
|---|
| Research Abstract |
In this project, an atomic force microscopy (AFM) instrument was developed to evaluate the reliability of fluorescence probe methods for molecular diffusion measurements in lipid bilayers. A sample holder developed in this project is usable in both the molecular-resolution AFM imaging and fluorescence measurements under the same condition. As the developed AFM instrument allows us to compare the data of molecular diffusion obtained by both methods, the capability should be useful for the reliability evaluation of fluorescence probe methods for molecular diffusion measurements.
|
Report
(3 results)
Research Products
(6 results)
