Evaluation of microbial community responses to mixed contaminant perturbation by using a functional genomics approach.

• Project/Area Number: 21810021
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Environmental impact assessment/Environmental policy
• Research Institution: Ehime University
• Principal Investigator: HAMAMURA Natsuko Ehime University, 沿岸環境科学研究センター・グローバルCOE, 准教授 (50554466)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000); Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000); Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
• Keywords: メタトランスクリプトーム / ヒ素 / 微生物生態 / 複合汚染 / 重金属耐性 / 機能ゲノミクス

Research Abstract

The aim of this study was to evaluate the overall effect of perturbation by mixed contaminants on microbial community in situ by detecting community-level gene expression responses using metatranscriptomic approach. The analysis of arsenic- and heavy metal-contaminated environmental samples revealed the specific expression of functional genes involved in metal resistance, which could potentially be targeted to assess toxic effect of pollutants in the environments.

Research Products

• [Journal Article] Distribution of aerobic arsenite oxidase genes within the Aquificales. (2011)
  • Author(s): Hamamura, N, R.E.Macur, Y.Liu, W.P.Inskeep, A-L.Reysenbach
  • Journal Title: Interdisciplinary studies on Environmental Chemistry Vol.3 Pages: 47-55
• [Journal Article] Metagenomes from high-temperature chemotrophic systems reveal importance of geochemical controls on microbial community structure and function. (2010)
  • Author(s): Inskeep, W.P., D.B.Rusch, Z.Jay, M.J.Herrgard, M.A.Kozubal, T.H.Richardson, R.E.Macur, N.Hamamura, 他11名
  • Journal Title: PLOS One Vol.5, No.3
  • Peer Reviewed
• [Journal Article] Distribution of aerobic arsenite oxidase genes within the Aquificales. (2010)
  • Author(s): Hamamura, N, R.E.Macur, Y.Liu, W.P.Inskeep, A-L.Reysenbach
  • Journal Title: Interdisciplinary studies on Environmental Chemistry. Volume: 3 Pages: 47-55
• [Journal Article] Metagenomes from high-temperature chemotrophic systems reveal importance of geochemical controls on microbial community structure and function (2010)
  • Author(s): Inskeep, W.P., D.B.Rusch, Z.Jay, M.J.Herrgard, M.A.Kozubal, T.H.Richardson, R.E.Macur, N.Hamamura, 他11名
  • Journal Title: PLOS One 5
  • Peer Reviewed
• [Presentation] 環境中ヒ素動態に影響を及ぼす微生物群集機能の解析 (2011)
  • Author(s): 濱村奈津子
  • Organizer: トピア科学シンポジウム「バイオレメディエーション微生物群の解析からデザイン化まで」講演要旨集,5.招待講演
  • Place of Presentation: 名古屋市
  • Year and Date: 2011-01-28
• [Presentation] 環境中ヒ素動態に影響を及ぼす微生物群集機能の解析 (2011)
  • Author(s): 濱村奈津子
  • Organizer: エコトピア科学シンポジウム「バイオレメディエーション:微生物群の解析からデザイン化まで」
  • Place of Presentation: 名古屋大学、愛知県
  • Year and Date: 2011-01-28
• [Presentation] Comparative meta-transcriptomic analysis of Aquificales-dominant terrestrial hot springs. (2011)
  • Author(s): Hamamura, N, J.Meneghin, A-L.Reysenbach
  • Organizer: TBI/INL/RCN-YNP Metagenomic Analysis Workshop
  • Place of Presentation: Jackson Hole, Wyoming, U.S.A
  • Year and Date: 2011-01-15
• [Presentation] Comparative meta-transcriptomic analysis of Aquificales-dominant terrestrial hot springs. (2011)
  • Author(s): Hamamura N., Meneghin, J., Reysenbach, A-L.
  • Organizer: TBI/INL/RCN- Metagenomic Analysis Workshop(講演要旨集5.)(ポスター発表)
  • Place of Presentation: Jackson Hole, Wyoming, U.S.A.
  • Year and Date: 2011-01-14
• [Presentation] Diversity and functional analysis of bacterial communities associated with soda lake in Khovsgol. (2010)
  • Author(s): Hamamura N., Itai, T., Liu, Y., Inskeep, W.P., Reysenbach, A-L.
  • Organizer: 日本微生物生態学会第26回大会,講演要旨集,127.ポスター発表
  • Place of Presentation: つくば市
  • Year and Date: 2010-11-25
• [Presentation] Diversity and functional analysis of bacterial communities associated with soda lake in Khovsgol, Mongolia. (2010)
  • Author(s): Hamamura, N, T.Itai, Y.Liu, W.P.Inskeep, A-L.Reysenbach
  • Organizer: 日本微生物生態学会第26回大会
  • Place of Presentation: 筑波大学、茨城県
  • Year and Date: 2010-11-25
• [Presentation] Comparative meta-transcriptomic analysis of Aquificales-dominant terrestrial hot springs. (2010)
  • Author(s): Hamamura N., Meneghin, J., Reysenbach, A-L.
  • Organizer: The 13th International Symposium in Microbial Ecology, Abstracts, CT35.002.口頭発表
  • Place of Presentation: Seattle, Washington, USA
  • Year and Date: 2010-08-26
• [Presentation] Comparative meta-transcriptomic analysis of Aquificales-dominant terrestrial hot springs. (2010)
  • Author(s): Hamamura, N, J.Meneghin, A-L.Reysenbach
  • Organizer: The 13^<th> International Symposium in Microbial Ecology
  • Place of Presentation: Seattle, Washington, U.S.A.
  • Year and Date: 2010-08-26
• [Presentation] Comparative community gene expression analysis of Aquificales-dominant geothermal springs. (2010)
  • Author(s): Hamamura N., Meneghin, J., Reysenbach, A-L.
  • Organizer: 第4回日本ゲノム微生物大会,講演要旨集,31.口頭発表
  • Place of Presentation: 福岡市
  • Year and Date: 2010-03-08
• [Presentation] Comparative community gene expression analysis of Aquificales-dominant geothermal springs (2010)
  • Author(s): Hamamura, N, J.Meneghin, A-L.Reysenbach
  • Organizer: 第4回日本ゲノム微生物学会年会
  • Place of Presentation: 九州大学、福岡県
  • Year and Date: 2010-03-08
• [Presentation] Comparative metatranscriptomics of Aquificales-dominant hot springs. (2009)
  • Author(s): Hamamura N., Meneghin, J., Reysenbach, A-L.
  • Organizer: 日本微生物生態学会第25回大会,講演要旨集,24.口頭発表
  • Place of Presentation: 広島市
  • Year and Date: 2009-11-21
• [Presentation] Comparative metatranscriptomics of Aquificales-dominant hot springs (2009)
  • Author(s): Hamamura, N, J.Meneghin, A-L.Reysenbach
  • Organizer: 日本微生物生態学会第25回大会
  • Place of Presentation: 広島大学、広島県
  • Year and Date: 2009-11-21
• [Remarks] アウトリーチ活動:微生物生態学会平成22年度大会の一環として催した一般向け公開講座(微生物観察教室)の実行委員として企画運営を行った。(2010年11月23日開催)
• [Remarks]
  • URL: http://ehime-u.cyber-earth.jp/g-coe2007/en/index.aspx