Methylated DNA binding protein MBD2/3 and its interacting proteins in Ciona intestinalis

• Project/Area Number: 21810039
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: 基礎ゲノム科学
• Research Institution: Institute for Developmental Research, Aichi Human Service Center
• Principal Investigator: SUZUKI Miho Institute for Developmental Research, Aichi Human Service Center, 発生障害学部, 研究員 (80548470)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000); Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000); Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
• Keywords: メチル化CpG結合タンパク質 / Ciona intestinalis / メチル化DNA結合タンパク質 / DNAメチル化 / メチルCpG結合タンパク質

Research Abstract

MBD2/3is highly expressed in early developmental stages of Ciona intestinalis. A knock down experiment depleting MBD2/3 protein in embryos caused severe developmental defects. The result indicated an important role of DNA methylation and methylated DNA binding protein in regulation of gene expression. Genome-wide distribution of MBD2/3 was limited to methylated gene body region. MBD2/3 possibly regulates expression of those more than 100gene targets.

Research Products

• [Journal Article] Maternal factor-mediated epigenetic gene silencing in the ascidian (2010)
  • Author(s): Yasunori Sasakura・Miho M.Suzuki・Akiko Hozumi・Kazuo Inaba・Nori Satoh
  • Journal Title: Ciona intestinalis Molecular Genetics and Genomics vol.283 Pages: 99-110
  • Peer Reviewed
• [Journal Article] Maternal factor-mediated epigenetic gene silencing in the ascidian Ciona intestinalis (2010)
  • Author(s): Yasunori Sasakura・Miho M.Suzuki・Akiko Hozumi・Kazuo Inaba・Nori Satoh
  • Journal Title: Molecular Genetics and Genomics 283 Pages: 99-110
  • Peer Reviewed
• [Presentation] gene body methylation-無脊椎動物からのアプローチ (2010)
  • Author(s): 鈴木美穂
  • Organizer: 第一回東海エピジェネティクスミーティング
  • Place of Presentation: 名古屋大学医学部
  • Year and Date: 2010-08-20
• [Presentation] gene body methylation-無脊椎動物からのアプローチ (2010)
  • Author(s): 鈴木美穂
  • Organizer: 東海エピジェネティクスミーティング
  • Place of Presentation: 名古屋大学医学部
  • Year and Date: 2010-08-20
• [Presentation] 次世代シークエンサーの概要と可能性 (2010)
  • Author(s): 鈴木美穂
  • Organizer: シンポジウム「次世代シークエンサーとDNA塩基配列情報の農業研究への展開」
  • Place of Presentation: 茨城大学農学部
  • Year and Date: 2010-02-26
• [Presentation] 次世代シークエンサーの概要と可能性 (2010)
  • Author(s): 鈴木美穂
  • Organizer: シンポジウム「次世代シークエンサーとDNA塩基配列情報の農業研究への展開」
  • Place of Presentation: 茨城大学
  • Year and Date: 2010-02-26
• [Remarks] ホームページ等
  • URL: http://www.inst-hsc.jp/