Construction of fluorescent ribonucleopeptide sensors for detecting signal transduction molecules.

• Project/Area Number: 21850018
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: Kyoto University
• Principal Investigator: FUKUDA Masatora Kyoto University, 理学部, 助教 (90526691)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,704,000 (Direct Cost: ¥2,080,000、Indirect Cost: ¥624,000); Fiscal Year 2010: ¥1,287,000 (Direct Cost: ¥990,000、Indirect Cost: ¥297,000); Fiscal Year 2009: ¥1,417,000 (Direct Cost: ¥1,090,000、Indirect Cost: ¥327,000)
• Keywords: リボヌクレオペプチド / バイオセンサー / 生理活性物質 / 分子設計 / 分子進化工学

Research Abstract

This research developed the methods of constructing fluorescent ribonucleopeptide (RNP) sensors for detecting signal transduction molecules. A covalently linked RNP sensor was designed by coupling an RNA subunit with a fluorophore -modified peptide subunit. The covalent linking strategy affords fluorescent RNP sensors for simultaneous detection of multiple ligands by monitoring each wavelength corresponding to the respective ligand.

Research Products

• [Journal Article] Structural aspects for substrate binding and fluorescence of the ATP-ribonucleopeptide receptors and sensors. (2010)
  • Author(s): Nakano S., Fukuda M., Mashima T., Katahira M., Morii T.
  • Journal Title: Ptptide Science (in press)
  • Peer Reviewed
• [Journal Article] Structural aspects for substrate binding and fluorescence of the ATP-binding ribonucleopeptide receptors and sensors (2010)
  • Author(s): Nakano S., Fukuda M., Mashima T., Katahira M., Morii T.
  • Journal Title: Ptptide Science 2010 Volume: In press
  • Peer Reviewed
• [Journal Article] Development of A Fluorescent Ribonucleopeptide Sensorfor Histamine. (2009)
  • Author(s): Fukuda M., Hayashi H., Hasegawa H., Morii T.
  • Journal Title: Trans.Mat.Res.Soc.Jpn. 34 Pages: 515-527
  • NAID: 130005004037
  • Peer Reviewed
• [Journal Article] Covalently linked fluorescent ribonucleopeptide sensors. (2009)
  • Author(s): Fukuda M., Liew F. F., Morii T.
  • Journal Title: Nucleic Acids Symp.Ser.(Oxf.) 53 Pages: 257-258
  • Peer Reviewed
• [Journal Article] Structural aspects for the function of ATP-bindingribonucleopeptide receptors. (2009)
  • Author(s): Nakano S., Fukuda M., Mashima T., Katahira M., Morii T.
  • Journal Title: Nucleic Acids Symp.Ser.(Oxf.) 53 Pages: 259-260
  • Peer Reviewed
• [Journal Article] Development of A Fluorescent Ribonucleopeptide Sensor for Histamine (2009)
  • Author(s): Fukuda, M., Hayashi, H., Hasegawa, H., Morii, T.
  • Journal Title: Transaction of the Materials Research Society of Japan 34 Pages: 525-527
  • NAID: 130005004037
  • Peer Reviewed
• [Journal Article] Covalently linked fluorescent ribonucleopeptide sensors (2009)
  • Author(s): Fukuda, M., Liew, F.F., Morii, T.
  • Journal Title: Nucleic Acids Symp.Ser.(Oxf.) 53 Pages: 257-258
• [Journal Article] Structural aspects for the function of ATP-binding ribonucleopeptide receptors (2009)
  • Author(s): Nakano, S., Fukuda, M., Mashima, T., Katahira, M., Morii, T.
  • Journal Title: Nucleic Acids Symp.Ser.(Oxf.) 53 Pages: 259-260
• [Presentation] The structural characterization of an ATP-binding ribonucleopeptide receptor. (2010)
  • Author(s): Nakano S., Fukuda M., Mashima T., Katahira M., Morii T.
  • Organizer: The 2010 International Chemical Congress of Pacific Basin Societies.
  • Place of Presentation: ホノル・アメリカ
  • Year and Date: 2010-12-17
• [Presentation] Development of ribonucleopeptide-based fluorescent sensors for dopamine. (2010)
  • Author(s): Liew F.F., Fukuda M., Tainaka, K., Nakano S., Morii T.
  • Organizer: The 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: ホノル・アメリカ
  • Year and Date: 2010-12-17
• [Presentation] Alteration of RNA editing profile of HTR2C mRNA depending on the dose double-stranded RNA adenosine deaminase. (2010)
  • Author(s): Tanaka Y., Kato S.,Fukuda M., Deshimaru M.
  • Organizer: The 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: ホノル・アメリカ
  • Year and Date: 2010-12-17
• [Presentation] The structural characterization of an ATP-binding ribonucleopeptide receptor (2010)
  • Author(s): Nakano S., Fukuda M., Mashima T., Katahira M., Morii T.
  • Organizer: The 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: ハワイ(ホノルル) アメリカ
  • Year and Date: 2010-12-17
• [Presentation] Development of ribonucleopeptide-based fluorescent sensors for dopamine (2010)
  • Author(s): Liew F.F., Fukuda M., Tainaka, K., Nakano S., Morii T.
  • Organizer: The 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: ハワイ(ホノルル) アメリカ
  • Year and Date: 2010-12-17
• [Presentation] Alteration of RNA editing profile of HTR2C mRNA depending on the dose of double-stranded RNA adenosine deaminase (2010)
  • Author(s): Tanaka Y., Kato S., Fukuda M., Deshimaru M.
  • Organizer: The 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: ハワイ(ホノルル) アメリカ
  • Year and Date: 2010-12-17
• [Presentation] セロトニン2C型受容体mRNAにおけるリボース2'-O-メチルがRNA編集よぼす影響、BMB2010 (2010)
  • Author(s): 喜多村春菜、鈴木智巳、山口彰太、田中泰圭、福田将虎、弟子丸正伸
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] セロトニン2C型受容体mRNAにおけるリボース2'-O-メチルがRNA編集におよぼす影響 (2010)
  • Author(s): 喜多村春菜、鈴木智巳、山口彰太、田中泰圭、福田将虎、弟子丸正伸
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会合同大会)
  • Place of Presentation: 兵庫(神戸ポートアイランド)
  • Year and Date: 2010-12-10
• [Presentation] Structural aspects for substrate binding and fluorescence of the ATP-ribonucleopeptide receptors and sensors. (2010)
  • Author(s): Nakano S., Fukuda M., Mashima T., Katahira M., Morii T.
  • Organizer: 5th International Peptide Symposium in conjunction with 47th Japanese Peptide Symposium
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2010-12-08
• [Presentation] セロトニン2C型受容体mRNAにおけるADAR1発現量依存的な編集パターン変化の解析、BMB2010 (2010)
  • Author(s): 田中泰圭、加藤卓、福田将虎、弟子丸正伸
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] Structural aspects for substrate binding and fluorescence of the ATP-binding ribonucleopeptide receptors and sensors (2010)
  • Author(s): Nakano S., Fukuda M., Mashima T., Katahira M., Morii T.
  • Organizer: 5th International Peptide Symposium in conjunction with 47th Japanese Peptide Symposium
  • Place of Presentation: 京都(京都国際会議場)
  • Year and Date: 2010-12-08
• [Presentation] セロトニン2C型受容体mRNAにおける編集パターンの決定機構 (2010)
  • Author(s): 田中泰圭、加藤卓、福田将虎、弟子丸正伸
  • Organizer: 第12回日本RNA学会年会
  • Place of Presentation: 一橋記念堂
  • Year and Date: 2010-07-27
• [Presentation] セロトニン2C型受容体mRNAにおける編集パターンの決定機構 (2010)
  • Author(s): 田中泰圭、加藤卓、福田将虎、弟子丸正伸
  • Organizer: 第12回日本RNA学会年会
  • Place of Presentation: 東京(一橋記念堂)
  • Year and Date: 2010-07-27
• [Presentation] ATP結合性リボヌクレオペプチドリセプターの構造と基質認識 (2010)
  • Author(s): 仲野瞬、福田将虎、真嶋司、片平正人、森井孝
  • Organizer: 日本化学会第90春期年会
  • Place of Presentation: 近畿大学
  • Year and Date: 2010-03-28
• [Presentation] 蛍光性リボヌクレオペプチド複合体によるカテコールアミン類の選択的認識 (2010)
  • Author(s): 劉芳芳、福田将虎、仲野瞬、田井中一貴、森井孝
  • Organizer: 日本化学会第90春期年会
  • Place of Presentation: 近畿大学
  • Year and Date: 2010-03-28
• [Presentation] Covalently linked fluorescent ribonucleopeptide sensors. (2009)
  • Author(s): Fukuda M., Liew F.F., Morii T.
  • Organizer: 第6回国際核酸化学シンポジウム
  • Place of Presentation: 高山文会館
  • Year and Date: 2009-09-29
• [Presentation] Covalently linked fluorescent ribonucleopeptide sensors (2009)
  • Author(s): Fukuda, M., Liew, F.F., Morii, T.
  • Organizer: 第6回国際核酸化学シンポジウム
  • Place of Presentation: 高山文化会館
  • Year and Date: 2009-09-29
• [Presentation] 蛍光性リボヌクレオペプチドセンサーを用いた複数の生理活性分子の同時検出 (2009)
  • Author(s): 福田将虎、Liew Fong-Fong、仲野瞬、森井孝
  • Organizer: 第24回生体機能関連化学シンポジウム,第12回バイオテクノロジー部会シンポジウム
  • Place of Presentation: 九州大学
  • Year and Date: 2009-09-15
• [Presentation] 蛍光性リボヌクレオペプチドセンサーを用いた複数の生理活性分子の同時検出 (2009)
  • Author(s): 福田将虎, Liew Fong-Fong, 仲野瞬, 森井孝
  • Organizer: 第24回生体機能関連化学シンポジウム, 第12回バイオテクノロジー部会シンポジウム
  • Place of Presentation: 九州大学
  • Year and Date: 2009-09-15
• [Presentation] 蛍光性リボヌクレオペプチドセンサーを用いた複数の標的分子の同時検出 (2009)
  • Author(s): 福田将虎, Liew Fong-Fong, 仲野瞬, 森井孝
  • Organizer: 第11回RNAミーティング
  • Place of Presentation: 新潟コンベンションセンター
  • Year and Date: 2009-07-28