Quantitative analysis of protein-protein interaction inside animal-derived cells
| Project/Area Number |
21850027
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | Konan University |
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Principal Investigator |
USUI Kenji Konan University, フロンティアサイエンス学部, 講師 (70543792)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥2,704,000 (Direct Cost: ¥2,080,000、Indirect Cost: ¥624,000)
Fiscal Year 2010: ¥1,287,000 (Direct Cost: ¥990,000、Indirect Cost: ¥297,000)
Fiscal Year 2009: ¥1,417,000 (Direct Cost: ¥1,090,000、Indirect Cost: ¥327,000)
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| Keywords | タンパク質 / 発現制御 / 細胞アレイ / ナノバイオ / タンパク質の自己相互作用 / ペプチド核酸(PNA) / 核酸の四重鎖構造 / アミロイド / 蛋白質 / 分析科学 / 細胞・組織 / プロテアーゼ |
|---|
| Research Abstract |
Construction of quantitative analysis system for protein-protein interaction inside animal-derived cells was attempted with controlling those protein expression levels using peptide nucleic acid (PNA)-peptide conjugates. We successfully achieved three indispensable basic elements for the system as follows: (1) construction of novel protein-expression control system, (2) construction of detection system for self-assembly reaction as a first step in establishing the analysis system of protein-protein interaction inside cells, (3) construction of cell array system for high-throughput analysis.
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Report
(3 results)
Research Products
(52 results)
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[Journal Article]2010
Author(s)
臼井健二、菊池卓哉、三原久和
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Journal Title
シングルセル解析の最前線"タンパク質・細胞分析用デザインペプチドチップ"(株式会社シーエムシー出版)
Pages: 17-24
Related Report
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[Journal Article]2009
Author(s)
臼井健二、富崎欣也、三原久和
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Journal Title
超分子サイエンス&テクノロジー"プロテインチップデバイス"(株式会社エヌ・ティー・エス)
Pages: 1067-1075
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