| Project/Area Number |
21850027
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Chemistry related to living body
|
|---|
| Research Institution | Konan University |
|---|
Principal Investigator |
USUI Kenji Konan University, フロンティアサイエンス学部, 講師 (70543792)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥2,704,000 (Direct Cost: ¥2,080,000、Indirect Cost: ¥624,000)
Fiscal Year 2010: ¥1,287,000 (Direct Cost: ¥990,000、Indirect Cost: ¥297,000)
Fiscal Year 2009: ¥1,417,000 (Direct Cost: ¥1,090,000、Indirect Cost: ¥327,000)
|
|---|
| Keywords | タンパク質 / 発現制御 / 細胞アレイ / ナノバイオ / タンパク質の自己相互作用 / ペプチド核酸(PNA) / 核酸の四重鎖構造 / アミロイド / 蛋白質 / 分析科学 / 細胞・組織 / プロテアーゼ |
|---|
| Research Abstract |
Construction of quantitative analysis system for protein-protein interaction inside animal-derived cells was attempted with controlling those protein expression levels using peptide nucleic acid (PNA)-peptide conjugates. We successfully achieved three indispensable basic elements for the system as follows: (1) construction of novel protein-expression control system, (2) construction of detection system for self-assembly reaction as a first step in establishing the analysis system of protein-protein interaction inside cells, (3) construction of cell array system for high-throughput analysis.
|
